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Efficacy of a Flow Cytometry Method for the Detection of an Anti-neutrophil Cytoplasmi Antibody in Ulcerative Colitis: A Comparison with an Indirect Immunofluorescence Method
http://hdl.handle.net/10191/1955
http://hdl.handle.net/10191/19550a89187e-eb69-4476-9caf-69361b7cc53b
名前 / ファイル | ライセンス | アクション |
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KJ00000007307.pdf (3.8 MB)
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2007-05-10 | |||||
タイトル | ||||||
タイトル | Efficacy of a Flow Cytometry Method for the Detection of an Anti-neutrophil Cytoplasmi Antibody in Ulcerative Colitis: A Comparison with an Indirect Immunofluorescence Method | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Efficacy of a Flow Cytometry Method for the Detection of an Anti-neutrophil Cytoplasmi Antibody in Ulcerative Colitis: A Comparison with an Indirect Immunofluorescence Method | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | ANCA | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | flow cytometry | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | indirect immunofluorescence microscopy | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | ulcerative colitis | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | departmental bulletin paper | |||||
著者 |
SATOH, Sadayuki
× SATOH, Sadayuki× ASAKURA, Hitoshi× HASEGAWA, Katsuhiko× OHTSUKA, Kazuo× YAMAGIWA, Satoshi× SUZUKI, Koji× NARISAWA, Rintarou× HONMA, Terasu× SUGIMURA, Kazuhito |
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内容記述タイプ | Abstract | |||||
内容記述 | Purpose : The anti-neutrophil cytoplasmic antibody (ANCA) in the serum of patients with ulcerative colitis (UC) can serve as a serological tool for making differential diagnosis from Crohn's disease (CD) and indicating a higher relapse rate in UC. This study was performed to clarify the clinical efficacy of the flow cytometry method for detecting ANCA in the serum of patients with UC, comparing this with an indirect immunofluorescence microscopy (IIF). Materials and Methods : Forty-two sera from 21 patients with active and inactive UC and seven sera from 7 patients with CD were obtained. Flow cytometry and IIF methods were employed to detect ANCA. For flow cytometry, indicator neutrophils were permeabilized by Saponin treatment, resuspended in the diluted sera, and incubated in FITC-conjugated goat antihuman immunoglobulin. These cells were analysed on a flow cytometer. For IIF, a commercial kit to detect ANCA was used. Results : Flow cytometry showed ANCA positivity in thirty-five of the 42 sera tested, and IIF in twenty-eight of the 42 sera tested. When compared with IIF, the flow cytometry for ANCA had a sensitivity of 96.5% and a specificity of 63.6%. ANCA positivity by flow cytometry and by IIF appeared in 23 and 19 of 28 sera tested in the entire colitis, respectively, and yielded almost similar results in left sided colitis. Positive rates and titers for ANCA by flow cytometry in the chronic continuous and relapse/remittent types of UC (intractable type) were significantly higher than those of the initial attack type of UC (non-intractable) and CD. Conclusion : ANCA positivity by flow cytometry was higher than that by IIF. The flow cytometry method is as clinically useful as the IIF method for detecting ANCA in UC because it is assessed objectively and quantitatively. | |||||
書誌情報 |
Acta medica et biologica en : Acta medica et biologica 巻 50, 号 1, p. 9-15, 発行日 2002-03 |
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出版者 | ||||||
出版者 | Niigata University School of Medicine | |||||
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収録物識別子タイプ | ISSN | |||||
収録物識別子 | 05677734 | |||||
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収録物識別子タイプ | NCID | |||||
収録物識別子 | AA00508361 | |||||
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値 | publisher |