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Expression and Intracellular Traffic of the Newly Synthesized FLAG-tagged M6a Glycoprotein
http://hdl.handle.net/10191/1937
http://hdl.handle.net/10191/19375ff3988b-7992-44fc-873c-946c0551a544
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
KJ00000007319.pdf (4.1 MB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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| 公開日 | 2007-05-10 | |||||
| タイトル | ||||||
| タイトル | Expression and Intracellular Traffic of the Newly Synthesized FLAG-tagged M6a Glycoprotein | |||||
| タイトル | ||||||
| タイトル | Expression and Intracellular Traffic of the Newly Synthesized FLAG-tagged M6a Glycoprotein | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | nerve growth cone | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | FLAG-tagged M6a | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Crelox system | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | axonal transport | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | departmental bulletin paper | |||||
| 著者 |
OBAYASHI, Hiroaki
× OBAYASHI, Hiroaki× KATAGIRI-ABE, Takako× ICHIMURA, Tohru× TANAKA, Hiroshi× GEJYO, Fumitake× ARAKAWA, Masaaki× KUWANO, Ryozo |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Mouse neuronal glycoprotein M6a was isolated from membrane proteins from the nerve growth cone-enriched fraction. M6a localization in the nerve growth cones was confirmed using a primary culture prepared from mouse heads at embryonic 10.5 days and polyclonal antibodies against the C-terminal portion of the M6a protein fused to GST. M6 expression was also observed in B103 neuroblastoma cells, but not in C6 astroglioma cells. To follow the intracellular migration of the M6a protein, we constructed a FLAG-tagged M6a expression plasmid. The monoclonal antibody against the FLAG epitope discriminated FLAG-tagged M6a from endogenous M6a, M6b and from PLP/DM20,which are related to M6a. The plasmid construct consisted of the neomycin resistant gene, the molony murine leukemia virus promoter flanked by loxP sites, and a FLAG-tagged M6a C-terminus. By tracing the FLAG intracellular expression, transfection of the Cre-recombinase plasmid resulted in the reduction of FLAG-tagged M6a expression and in immunoreactive FLAG-tagged M6a in the growth cone. Treatment of cells with brefeldin A caused the distributed M6a-FLAG to collapse around the nucleus and to have a staining pattern similar to that of BiP and PDI, which are endoplasmic reticulum or Golgi-associated markers. M6a transportation to the cell surface and neurites was inhibited by vinblastine. In addition, the synthesized M6a accumulated in the perinuclear vesicles and the constricted region between the soma and neurites. In contrast with the results with vinblastine, M6a was detected throughout the cell in the presence of cytochalasin E. These observations indicated that M6a moves on microtubules, but not on actin filaments. | |||||
| 書誌情報 |
Acta medica et biologica en : Acta medica et biologica 巻 50, 号 2, p. 71-82, 発行日 2002-06 |
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| 出版者 | ||||||
| 出版者 | Niigata University School of Medicine | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 05677734 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA00508361 | |||||
| 著者版フラグ | ||||||
| 値 | publisher | |||||
