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Nosocomial Outbreak-Derived Serratia marcescens : Rapid and Quantitative Detection of the Agent in the Blood by a Real Time PCR Assay
http://hdl.handle.net/10191/1828
http://hdl.handle.net/10191/1828dd4f3063-3bbd-48ef-8fbc-9c14e59b9138
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
KJ00000007344.pdf (2.6 MB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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| 公開日 | 2007-05-10 | |||||
| タイトル | ||||||
| タイトル | Nosocomial Outbreak-Derived Serratia marcescens : Rapid and Quantitative Detection of the Agent in the Blood by a Real Time PCR Assay | |||||
| タイトル | ||||||
| タイトル | Nosocomial Outbreak-Derived Serratia marcescens : Rapid and Quantitative Detection of the Agent in the Blood by a Real Time PCR Assay | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Serratia marcescens | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Real time (TaqMan) PCR | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Bacteremia | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | departmental bulletin paper | |||||
| 著者 |
IWAYA, Akira
× IWAYA, Akira× NAKAGAWA, Saori× IWAKURA, Nobuhiro× TANEIKE, Ikue× HATAKEYAMA, Katsuyoshi× YAMAMOTO, Tatsuo |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | There have recently been three large nosocomial outbreaks of Serratia marcescens with serious septicaemia and high mortality in Japan. In these blood stream infections, several patients died before the blood culture results were obtained, suggesting that rapid and sensitive methods are needed to detect S. marcescens in the blood. In this study, we examined a rapid DNA diagnosis of outbreak-derived S. marcescens in blood samples by a real time (TaqMan) PCR assay. The minimum level of sensitivity achieved was 5×1O^1 CFU/ml in a buffer, and 1×10^2 CFU/ml in blood samples. The real time PCR assay was completed within 3 h from only 200 μl of blood samples. There was a linear correlation between the bacterial cell number (in a range of 10^2 to 10^7 CFU/ml) and the minimum number of cycling to yield positive results in the assay. The data suggest that a real time (TaqMan) PCR assay is useful for the rapid and quantitative detection of S. marcescens in blood samples. | |||||
| 書誌情報 |
Acta medica et biologica en : Acta medica et biologica 巻 50, 号 4, p. 185-190, 発行日 2002-12 |
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| 出版者 | ||||||
| 出版者 | Niigata University School of Medicine | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 05677734 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA00508361 | |||||
| 著者版フラグ | ||||||
| 値 | publisher | |||||
