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  1. 0 資料タイプ別
  2. 03 紀要論文
  1. 250 大学院医歯学総合研究科(医)
  2. 20 紀要
  3. 01 Acta medica et biologica
  4. Vol.51 No.1

Effects of EGF on Cell Migration, and the Association of FAK and Paxillin to the Cytoskeleton

http://hdl.handle.net/10191/1893
http://hdl.handle.net/10191/1893
44ded743-20c2-4ebb-903e-fee860d1815f
名前 / ファイル ライセンス アクション
KJ00000007358.pdf KJ00000007358.pdf (2.7 MB)
Item type 紀要論文 / Departmental Bulletin Paper(1)
公開日 2007-05-10
タイトル
タイトル Effects of EGF on Cell Migration, and the Association of FAK and Paxillin to the Cytoskeleton
タイトル
言語 en
タイトル Effects of EGF on Cell Migration, and the Association of FAK and Paxillin to the Cytoskeleton
言語
言語 eng
キーワード
主題Scheme Other
主題 EGF
キーワード
主題Scheme Other
主題 actin stress fibers
キーワード
主題Scheme Other
主題 focal adhesion
キーワード
主題Scheme Other
主題 FAK
キーワード
主題Scheme Other
主題 paxillin
キーワード
主題Scheme Other
主題 tyrosine-phosphorylation
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ departmental bulletin paper
著者 CHIBA, Hitomi

× CHIBA, Hitomi

WEKO 52070

CHIBA, Hitomi

Search repository
KAZAMA, Takashi

× KAZAMA, Takashi

WEKO 52071

KAZAMA, Takashi

Search repository
YAMAMOTO, Yoko

× YAMAMOTO, Yoko

WEKO 52072

YAMAMOTO, Yoko

Search repository
SUYAMA, Takayuki

× SUYAMA, Takayuki

WEKO 52073

SUYAMA, Takayuki

Search repository
ITO, Masaaki

× ITO, Masaaki

WEKO 52074

ITO, Masaaki

Search repository
抄録
内容記述タイプ Abstract
内容記述 We investigated the effects of epidermal growth factor (EGF) on cell migration, and the association of focal adhesion kinase (FAK) and paxillin to cytoskeleton. A cell line of squamous cell carcinoma (SCC) of the uterine cervix, named Caski, was used. We examined the effects of different concentrations of EGF (0, 1, 10 and 100 ng/ml) on cell migration. Wound closure assay showed that cell migration within 4 h had a biphasic dose-response curve on the concentration of EGF, and that cell migration was stimulated at 1 and 10 ng/ml, with a maximum at 10 ng/ml, and suppressed at 100 ng/ml among the concentrations examined. Immunoblot and immunoprecipitation assays of whole cell lysates obtained at 20 min after the addition of EGF demonstrated that the phosphotyrosine level of FAK and paxillin had also a biphasic response curve dependent on EGF concentration, and a maximum level was observed at 10 ng/ml among the concentrations employed. To examine the amount of cytoskeleton-associated fractions of these proteins, they were extracted after a rinse in non-ionic detergent, and analyzed by immunoblot assay. At 30 min the amount of FAK and paxillin did not differ among the concentrations examined. At 90 min, however, the amount of both proteins revealed a reverse biphasic dose-response curve, with a maximal reduction at 10 ng/ml among the concentrations examined. Integrins and actinin were not changed at each concentration of EGF examined. Immunofluorescence microscopy showed that at 30 min the expression of actin stress fibers (ASF) did not differ among cells treated with each concentration of EGF. At 90 min the ratio of cells that expressed a small number of ASF and focal adhesions (FA) was significantly increased at 10 ng/ml. Although the ratio of cells that expressed large numbers of these structures was not significantly altered, ASF were gathered at the center of cells and tangled at 100 ng/ml. These data suggest that the modulation of cytoskeletal organization by EGF plays a role in the EGF-induced stimulation of cell migration.
書誌情報 Acta medica et biologica
en : Acta medica et biologica

巻 51, 号 1, p. 31-41, 発行日 2003-03
出版者
出版者 Niigata University School of Medicine
ISSN
収録物識別子タイプ ISSN
収録物識別子 05677734
書誌レコードID
収録物識別子タイプ NCID
収録物識別子 AA00508361
著者版フラグ
値 publisher
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