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  1. 0 資料タイプ別
  2. 03 紀要論文
  1. 250 大学院医歯学総合研究科(医)
  2. 20 紀要
  3. 01 Acta medica et biologica
  4. Vol.51 No.1

The Genomic Organization, Alternative Splicing, and Promotor Assay of the Mouse Ankhzn Gene

http://hdl.handle.net/10191/1943
http://hdl.handle.net/10191/1943
9f699083-c891-49be-bf14-93144cc9efba
名前 / ファイル ライセンス アクション
KJ00000007356.pdf KJ00000007356.pdf (4.7 MB)
Item type 紀要論文 / Departmental Bulletin Paper(1)
公開日 2007-05-10
タイトル
タイトル The Genomic Organization, Alternative Splicing, and Promotor Assay of the Mouse Ankhzn Gene
タイトル
タイトル The Genomic Organization, Alternative Splicing, and Promotor Assay of the Mouse Ankhzn Gene
言語 en
言語
言語 eng
キーワード
主題Scheme Other
主題 alternative splicing
キーワード
主題Scheme Other
主題 RT-PCR
キーワード
主題Scheme Other
主題 intron
キーワード
主題Scheme Other
主題 exon
キーワード
主題Scheme Other
主題 FYVE finger
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ departmental bulletin paper
著者 MARUYAMA, Hiroshi

× MARUYAMA, Hiroshi

WEKO 52060

MARUYAMA, Hiroshi

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KURIYAMA, Hideyuki

× KURIYAMA, Hideyuki

WEKO 52061

KURIYAMA, Hideyuki

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ISHII, Naoya

× ISHII, Naoya

WEKO 52062

ISHII, Naoya

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ITO, Kazuhisa

× ITO, Kazuhisa

WEKO 52063

ITO, Kazuhisa

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ODANI, Shoji

× ODANI, Shoji

WEKO 52064

ODANI, Shoji

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KUWANO, Ryozo

× KUWANO, Ryozo

WEKO 52065

KUWANO, Ryozo

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抄録
内容記述タイプ Abstract
内容記述 We have previously established a GT3-12 mouse line in which a novel gene was trapped by a promoterless gene trap (GT) vector. The gene was designated as Ankhzn because of the presence of 17 ankyrin-repeats and a zinc-finger domain FYVE. In this study, we characterize the genomic organization of the mouse Ankhzn gene by analyzing two overlapping P1 (bacteriophage P1 cloning system) clones and a single bacterial artificial chromosome (BAC) clone. The Ankhzn gene spans more than 95 kb and comprises 25 exons, where the splice site conforms to the GT-AG rule except for the GC splice donor site instead of GT of intron 4. This GC splice donor site and several junctions are conserved between the mouse Ankhzn and the human ANKHZN. Furthermore, results of RT-PCR revealed that the 49 bp segment at the 3' end of exon 9 is alternatively spliced to generate two splice variants. One splice variant is a long form that has a calculated molecular mass of 130 kDa. The other is a short form that has a molecular mass of 50 kDa resulting from a frameshift leading to premature termination within exon 10. Results of RT-PCR analysis showed that the Ankhzn long form was expressed much more strongly than the short form in all tissues and developmental stages examined. The Ankhzn gene was identified as a single-copy gene by Southern blot analysis. Examination of the T31 Mouse Radiation Hybrid Database RH Chr 11 Public Map Data at the Jackson Laboratory showed its localization to mouse chromosome 11 which has high synteny to human chromosome 17. A promoter assay with a luciferase reporter gene revealed that an approximately 200 bp upstream region to the transcription start site is essential for the transcription of Ankhzn.
書誌情報 Acta medica et biologica
en : Acta medica et biologica

巻 51, 号 1, p. 13-24, 発行日 2003-03
出版者
出版者 Niigata University School of Medicine
ISSN
収録物識別子タイプ ISSN
収録物識別子 05677734
書誌レコードID
収録物識別子タイプ NCID
収録物識別子 AA00508361
著者版フラグ
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