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  1. 0 資料タイプ別
  2. 03 紀要論文
  1. 250 大学院医歯学総合研究科(医)
  2. 20 紀要
  3. 01 Acta medica et biologica
  4. Vol.55 No.1

Localization of Tyrosine-Phosphorylated Proteins in the Normal Rat Kidney

http://hdl.handle.net/10191/5239
http://hdl.handle.net/10191/5239
6e571275-50f5-4c36-a765-4fd527d734e8
名前 / ファイル ライセンス アクション
17_0012.pdf 17_0012.pdf (1.2 MB)
Item type 紀要論文 / Departmental Bulletin Paper(1)
公開日 2008-04-08
タイトル
タイトル Localization of Tyrosine-Phosphorylated Proteins in the Normal Rat Kidney
タイトル
タイトル Localization of Tyrosine-Phosphorylated Proteins in the Normal Rat Kidney
言語 en
言語
言語 eng
キーワード
主題Scheme Other
主題 kidney
キーワード
主題Scheme Other
主題 glomerulus
キーワード
主題Scheme Other
主題 tyrosine phosphorylation
キーワード
主題Scheme Other
主題 podocyte
キーワード
主題Scheme Other
主題 foot process
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ departmental bulletin paper
著者 Zhang, Ying

× Zhang, Ying

WEKO 51614

Zhang, Ying

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Yoshida, Yutaka

× Yoshida, Yutaka

WEKO 51615

Yoshida, Yutaka

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Xu, Bo

× Xu, Bo

WEKO 51616

Xu, Bo

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Nameta, Masaaki

× Nameta, Masaaki

WEKO 51617

Nameta, Masaaki

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Miyamoto, Masahito

× Miyamoto, Masahito

WEKO 51618

Miyamoto, Masahito

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Yaoita, Eishin

× Yaoita, Eishin

WEKO 51619

Yaoita, Eishin

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Yamamoto, Tadashi

× Yamamoto, Tadashi

WEKO 51620

Yamamoto, Tadashi

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抄録
内容記述タイプ Abstract
内容記述 Protein tyrosine phosphorylation is responsible for activating or repressing the activity of proteins involved in numerous signal transduction pathways in a living organism. Using immunochemical methods with two well characterized monoclonal anti-phosphotyrosine antibodies, we examined the distribution and localization of tyrosinephosphorylated proteins expressed in the three major compartments of the normal rat kidney: the glomerulus, cortex, and medulla. Western blotting analysis revealed that tyrosine-phosphorylated proteins were predominantly expressed in the glomerulus, compared with the cortex and medulla. The intensity of tyrosine-phosphorylated protein bands was augmented by using orthovanadate - an inhibitor of protein tyrosine phosphatase - during sample preparation, and the bands were undetectable when the antibodies were absorbed with phosphotyrosine but not with phosphoserine or phosphothreonine. Immunofluorescence microscopy indicated that the phosphotyrosine staining was intense along glomerular capillary walls and sparse along parts of the tubuli. Immunoelectron microscopy further showed that phosphotyrosine immunoreactivity was predominantly localized at the basal membranes of podocyte foot processes. In addition, some immunogold labeling was also observed at cell-matrix attachment sites of glomerular endothelial cells and mesangial cells as well as at basal membranes of epithelial cells of proximal tubules, distal tubules, and collecting ducts. In conclusion, tyrosine-phosphorylated proteins are predominantly and constitutively expressed in the normal rat glomerulus, especially in the basal membranes of podocyte foot processes, suggesting tyrosinephosphorylated proteins could play an important role in maintaining the unique organization of foot processes and in the glomerular ultra filtration there.
書誌情報 Acta medica et biologica
en : Acta medica et biologica

巻 55, 号 1, p. 1-7, 発行日 2007-03
出版者
出版者 Niigata University School of Medicine
ISSN
収録物識別子タイプ ISSN
収録物識別子 05677734
書誌レコードID
収録物識別子タイプ NCID
収録物識別子 AA00508361
著者版フラグ
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