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STUDY OF LONG-RANGE SURFACE PLASMON RESONANCE BIOSENSORS FOR ABO BLOOD TYPING
http://hdl.handle.net/10191/50332
http://hdl.handle.net/10191/503324aa40e1c-478c-4f2b-9a2a-d215c7346cc4
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本文 (4.0 MB)
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要旨 (91.5 kB)
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| Item type | 学位論文 / Thesis or Dissertation(1) | |||||
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| 公開日 | 2018-07-06 | |||||
| タイトル | ||||||
| タイトル | STUDY OF LONG-RANGE SURFACE PLASMON RESONANCE BIOSENSORS FOR ABO BLOOD TYPING | |||||
| タイトル | ||||||
| タイトル | STUDY OF LONG-RANGE SURFACE PLASMON RESONANCE BIOSENSORS FOR ABO BLOOD TYPING | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Long range-surface plasmon resonance | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Transmission surface plasmon resonance | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Red blood cell | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | ABO blood grouping | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Optical biosensor | |||||
| 資源タイプ | ||||||
| 資源 | http://purl.org/coar/resource_type/c_46ec | |||||
| タイプ | thesis | |||||
| その他のタイトル | ||||||
| その他のタイトル | ABO 式血液型測定のための長距離伝搬表面プラズモン共鳴バイオセンサーに関する研究 | |||||
| 著者 |
TANGKAWSAKUL, WANIDA
× TANGKAWSAKUL, WANIDA |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | In this study, I would like to propose new techniques for blood grouping application. Due to the advantage of optical biosensors such as high sensitivity, specificity, simple setup and rapid detection, they have been applied to a variety of biomolecules. Optical biosensors have also been interested in blood grouping application. In this work, I divided in two parts of feasibility studies which were developed as an optical biosensor for blood grouping based on two techniques. In the first study, I demonstrate a long-range surface plasmon resonance (LR-SPR) biosensor for the detection of whole cell by captured antigens A and B on the surface of red blood cells (RBCs) as a model. The LR-SPR sensor chip consists of high-refractive index glass, a Cytop film layer, and a thin gold (Au) film, which makes the evanescent field intensity and the penetration depth longer than conventional SPR. Therefore, the LR-SPR biosensor has improved capability for detecting large analytes, such as RBCs. The antibodies specific to blood group A and group B (Anti-A and Anti-B) are covalently immobilized on a grafting self-assembled monolayer (SAM)/Au surface on the biosensor. For blood typing, RBC samples can be detected by the LR-SPR biosensor through a change in the refractive index. I determined that the results of blood typing using the LR-SPR biosensor were consistent with the results obtained from the agglutination test. I obtained the lowest detection limits of 1.58 × 10^5 cells/ml for RBC-A and 3.83 × 10^5 cells/ml for RBC-B, indicating that the LR-SPR chip has a higher sensitivity than conventional SPR biosensors (3.3 × 10^8 cells/ml). The surface of the biosensor can be efficiently regenerated using 20 mM NaOH. The second study, a transmission surface plasmon resonance (T-SPR) technique for ABO blood typing was proposed based on the immunological interaction between surface antigen of the red blood cell (RBC) and immobilized antibody. The antibody specific to blood group A (Anti-A) was covalently immobilized on carboxylic acid on gold grating surface. The RBC samples were detected on the immobilized anti-A where the blood typing was determined from the change in the intensity of the transmission peak by TSPR technique. The maximum TSPR excitation was at an incident angle 35°. All the results of blood typing was all consistent with the results obtained from agglutination test. In summary, as the performance, sensitivity, simply experimental setup and utility of LR-SPR and T-SPR technique, it can easily be applied for ABO blood group typing. | |||||
| 内容記述 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 学位の種類: 博士（工学）. 報告番号: 甲第4469号. 学位記番号: 新大院博（工）甲第476号. 学位授与年月日: 平成30年3月23日 | |||||
| 内容記述 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | CHAPTER 4: International Journal of Analytical Chemistry. Volume 2016, Article ID 1432781. | |||||
| 書誌情報 | p. 1-84, 発行日 2018-03-23 | |||||
| 出版者 | ||||||
| 出版者 | 新潟大学 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | info:doi/10.1155/2016/1432781 | |||||
| 著者版フラグ | ||||||
| 値 | ETD | |||||
| 学位名 | ||||||
| 学位名 | 博士（工学） | |||||
| 学位授与機関 | ||||||
| 学位授与機関名 | 新潟大学 | |||||
| 学位授与年月日 | ||||||
| 学位授与年月日 | 2018-03-23 | |||||
| 学位授与番号 | ||||||
| 学位授与番号 | 13101甲第4469号 | |||||
| 学位記番号 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 新大院博（工）甲第476号 | |||||
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Cite as
TANGKAWSAKUL, WANIDA, 2018, STUDY OF LONG-RANGE SURFACE PLASMON RESONANCE BIOSENSORS FOR ABO BLOOD TYPING: 新潟大学, p. 1–84.
