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The Role of Mutation Rates of GNAQ or GNA11 in Cases of Uveal Melanoma in Japan
http://hdl.handle.net/10191/50415
http://hdl.handle.net/10191/50415af3a3f5d-d00a-4d83-8219-59ada2af93ae
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本文 (294.0 kB)
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要旨 (678.3 kB)
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| Item type | 学位論文 / Thesis or Dissertation(1) | |||||
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| 公開日 | 2018-07-26 | |||||
| タイトル | ||||||
| タイトル | The Role of Mutation Rates of GNAQ or GNA11 in Cases of Uveal Melanoma in Japan | |||||
| タイトル | ||||||
| タイトル | The Role of Mutation Rates of GNAQ or GNA11 in Cases of Uveal Melanoma in Japan | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | uveal melanoma | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | GNAQ/11 mutation | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | cell types | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Ki-67 | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_46ec | |||||
| 資源タイプ | thesis | |||||
| その他のタイトル | ||||||
| その他のタイトル | 日本人ぶどう膜悪性黒色腫症例におけるGNAQ遺伝子及びGNA11遺伝子の変異率と遺伝子変異と臨床病理学的所見との関連についての検討 | |||||
| 著者 |
Ominato, Jun
× Ominato, Jun |
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| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 51203 | |||||
| 姓名 | 大湊, 絢 | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | GNAQ and GNA11 mutations are thought to be important for the tumorigenesis of uveal melanoma. Although previous studies have reported on mutation rates in cases of uveal melanoma, presently, no such report for the Japanese population exists. In this study, we examined the frequency of GNAQ and GNA11 somatic mutations in cases of uveal melanoma in Japan and their relationship with clinicopathologic features or Ki-67-positive cell rates (Ki-67 labelingindex: Ki-67 LI) using immunofluorescence methods. The study involved 19 cases of uveal melanoma. We extracted the template DNA from formalin-fixed, paraffin-embedded specimens using a DNA extraction kit. We amplified the DNA sequences of GNAQ and GNA11 using polymerase chain reaction and analyzed mutations by direct sequencing. We evaluated Ki-67 LI using immunofluorescence methods. The frequencies of GNAQ and GNA11 somatic mutations were 26.3% (5/19) and 31.6% (6/19), respectively. The GNAQ and GNA11 mutations were mutually exclusive, as indicated in previous reports. The frequency of GNA11 mutations was significantly higher in epithelioid cells; however, no significant association between GNAQ mutations and cell type was evident, and there was no significant difference in Ki-67 LI between the mutation-positive and mutation-negative tumors. GNAQ and GNA11 mutations were identified in cases of uveal melanoma in Japan, although at lower frequencies than in white counterparts. The mutation frequency of GNA11 was significantly higher in epithelioid cells. | |||||
| 内容記述 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 学位の種類: 博士（医学）. 報告番号: 甲第4390号. 学位記番号: 新大院博（医）甲第789号. 学位授与年月日: 平成30年3月23日 | |||||
| 内容記述 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Applied Immunohistochemistry & Molecular Morphology. 2017 . Publish Ahead of Print. | |||||
| 書誌情報 | p. 1-6, 発行日 2018-03-23 | |||||
| 出版者 | ||||||
| 出版者 | 新潟大学 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | info:doi/10.1097/PAI.0000000000000505 | |||||
| 著者版フラグ | ||||||
| 値 | ETD | |||||
| 学位名 | ||||||
| 学位名 | 博士（医学） | |||||
| 学位授与機関 | ||||||
| 学位授与機関名 | 新潟大学 | |||||
| 学位授与年月日 | ||||||
| 学位授与年月日 | 2018-03-23 | |||||
| 学位授与番号 | ||||||
| 学位授与番号 | 13101甲第4390号 | |||||
| 学位記番号 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 新大院博（医）甲第789号 | |||||
