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Identification of cellular genes showing differential expression associated with hepatitis B virus infection
http://hdl.handle.net/10191/48162
http://hdl.handle.net/10191/4816203194301-eec3-43fd-a644-bd83963219b7
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本文 (1.2 MB)
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要旨 (187.0 kB)
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| Item type | 学位論文 / Thesis or Dissertation(1) | |||||
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| 公開日 | 2017-11-07 | |||||
| タイトル | ||||||
| タイトル | Identification of cellular genes showing differential expression associated with hepatitis B virus infection | |||||
| タイトル | ||||||
| タイトル | Identification of cellular genes showing differential expression associated with hepatitis B virus infection | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Hepatitis B virus | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Differential gene expression | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Hepatocellular carcinoma | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Gene expression signature | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Adipose most abundant 2 | |||||
| 資源タイプ | ||||||
| 資源 | http://purl.org/coar/resource_type/c_46ec | |||||
| タイプ | thesis | |||||
| その他のタイトル | ||||||
| その他のタイトル | HBV感染に伴い特異的に発現変動する宿主遺伝子の同定 | |||||
| 著者 |
Fukuhara, Yasuo
× Fukuhara, Yasuo |
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| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 51181 | |||||
| 姓名 | 福原, 康夫 | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | AIM: To investigate the impact of hepatitis B virus (HBV) infection on cellular gene expression, by conducting both in vitro and in vivo studies. METHODS: Knockdown of HBV was targeted by stable expression of short hairpin RNA (shRNA) in huH-1 cells. Cellular gene expression was compared using a human 30K cDNA microarray in the cells and quantified by real-time reverse transcription-polymerase chain reaction (RT-PCR) (qRT-PCR) in the cells, hepatocellular carcinoma (HCC) and surrounding non-cancerous liver tissues (SL). RESULTS: The expressions of HBsAg and HBx protein were markedly suppressed in the cells and in HBx transgenic mouse liver, respectively, after introduction of shRNA. Of the 30K genes studied, 135 and 103 genes were identified as being down- and up-regulated, respectively, by at least twofold in the knockdown cells. Functional annotation revealed that 85 and 62 genes were classified into four up-regulated and five downregulated functional categories, respectively. When gene expression levels were compared between HCC and SL, eight candidate genes that were confirmed to be up- or down-regulated in the knockdown cells by both microarray and qRT-PCR analyses were not expressed as expected from HBV reduction in HCC, but had similar expression patterns in HBV- and hepatitis C virus-associated cases. In contrast, among the eight genes, only APM2 was constantly repressed in HBV non-associated tissues irrespective of HCC or SL. CONCLUSION: The signature of cellular gene expression should provide new information regarding the pathophysiological mechanisms of persistent hepatitis and hepatocarcinogenesis that are associated with HBV infection. | |||||
| 内容記述 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 学位の種類: 博士（医学）. 報告番号: 乙第2213号. 学位記番号: 新大博（医）乙第1788号. 学位授与年月日: 平成29年9月20日 | |||||
| 内容記述 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | World Journal of Hepatology. 2012, 4(4), 139-148. | |||||
| 書誌情報 | 発行日 2017-09-20 | |||||
| 出版者 | ||||||
| 出版者 | 新潟大学 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | info:doi/10.4254/wjh.v4.i4.139 | |||||
| 権利 | ||||||
| 権利情報 | (C) 2012 Baishideng. All rights reserved. | |||||
| 著者版フラグ | ||||||
| 値 | ETD | |||||
| 学位名 | ||||||
| 学位名 | 博士（医学） | |||||
| 学位授与機関 | ||||||
| 学位授与機関名 | 新潟大学 | |||||
| 学位授与年月日 | ||||||
| 学位授与年月日 | 2017-09-20 | |||||
| 学位授与番号 | ||||||
| 学位授与番号 | 13101乙第2213号 | |||||
| 学位記番号 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 新大博（医）乙第1788号 | |||||
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Cite as
Fukuhara, Yasuo, 2017, Identification of cellular genes showing differential expression associated with hepatitis B virus infection: 新潟大学.
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