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  1. 0 資料タイプ別
  2. 02 学位論文
  1. 250 大学院医歯学総合研究科(医)
  2. 60 博士学位論文
  3. 10 博士学位論文

Effects of Fibrotic Tissue on Liver-targeted Hydrodynamic Gene Delivery.

http://hdl.handle.net/10191/47583
http://hdl.handle.net/10191/47583
6c94c34a-eb3a-437b-a86e-ff683f11db3f
名前 / ファイル ライセンス アクション
h28nmk730.pdf 本文 (1.3 MB)
h28nmk730_a.pdf 要旨 (197.7 kB)
Item type 学位論文 / Thesis or Dissertation(1)
公開日 2018-09-11
タイトル
タイトル Effects of Fibrotic Tissue on Liver-targeted Hydrodynamic Gene Delivery.
タイトル
言語 en
タイトル Effects of Fibrotic Tissue on Liver-targeted Hydrodynamic Gene Delivery.
言語
言語 eng
キーワード
主題Scheme Other
主題 gene therapy
キーワード
主題Scheme Other
主題 hydrodynamic gene delivery
キーワード
主題Scheme Other
主題 liver fibrosis
キーワード
主題Scheme Other
主題 MMP13
キーワード
主題Scheme Other
主題 nonviral gene delivery
資源タイプ
資源 http://purl.org/coar/resource_type/c_46ec
タイプ thesis
その他のタイトル
その他のタイトル 肝臓選択的ハイドロダイナミック遺伝子導入法において、線維組織が導入効率に与える影響
著者 Kobayashi, Yuji

× Kobayashi, Yuji

WEKO 50963

Kobayashi, Yuji

Search repository
著者別名
識別子 50964
識別子Scheme WEKO
姓名 小林, 雄司
抄録
内容記述タイプ Abstract
内容記述 Hydrodynamic gene delivery is a common method for gene transfer to the liver of small animals, and its clinical applicability in large animals has been demonstrated. Previous studies focused on functional analyses of therapeutic genes in animals with normal livers and little, however, is known regarding its effectiveness and safety in animals with liver fibrosis. Therefore, this study aimed to examine the effects of liver fibrosis on hydrodynamic gene delivery efficiency using a rat liver fibrosis model. We demonstrated for the first time, using pCMV-Luc plasmid, that this procedure is safe and that the amount of fibrotic tissue in the liver decreases gene delivery efficiency, resulting in decrease in luciferase activity depending on the volume of fibrotic tissue in the liver and the number of hepatocytes that are immunohistochemically stained positive for transgene product. We further demonstrate that antifibrotic gene therapy with matrix metalloproteinase-13 gene reduces liver fibrosis and improves efficiency of hydrodynamic gene delivery. These results demonstrate the negative effects of fibrotic tissue on hydrodynamic gene delivery and its recovery by appropriate antifibrotic therapy
内容記述
内容記述タイプ Other
内容記述 学位の種類: 博士(医学). 報告番号: 甲第4252号. 学位記番号: 新大院博(医)甲第730号. 学位授与年月日: 平成29年3月23日
内容記述
内容記述タイプ Other
内容記述 Molecular Therapy. Nucleic Acids 5(8) e359 2016
書誌情報 発行日 2017-03-23
出版者
出版者 新潟大学
DOI
識別子タイプ DOI
関連識別子 info:doi/10.1038/mtna.2016.63
権利
権利情報 © 2016 Elsevier Inc. All rights reserved
著者版フラグ
値 ETD
学位名
学位名 博士(医学)
学位授与機関
学位授与機関名 新潟大学
学位授与年月日
学位授与年月日 2017-03-23
学位授与番号
学位授与番号 13101甲第4252号
学位記番号
内容記述タイプ Other
内容記述 新大院博(医)甲第730号
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