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Cell mosaicism of brain tissues makes their detection and validation difficult, however. In the present study, we analyzed gene dosage alterations in brain DNA of schizophrenia patients and compared those with the copy number variations (CNVs) identified in schizophrenia patients as well as with those in Asian lymphocyte DNA and attempted to obtain hints at the pathological contribution of cytogenomic instability in schizophrenia. Results: Brain DNA was extracted from postmortem striatum of schizophrenia patients and control subjects (n = 48 each) and subjected to the direct two color microarray analysis that limits technical data variations. Disease-associated biases of relative DNA doses were statistically analyzed with Bonferroni\u0027s compensation on the premise of brain cell mosaicism. We found that the relative gene dosage of 85 regions significantly varied among a million of probe sites. In the candidate CNV regions, 26 regions had no overlaps with the common CNVs found in Asian populations and included the genes (i.e., ANTXRL, CHST9, DNM3, NDST3, SDK1, STRC, SKY) that are associated with schizophrenia and/or other psychiatric diseases. The majority of these candidate CNVs exhibited high statistical probabilities but their signal differences in gene dosage were less than 1.5-fold. For test evaluation, we rather selected the other 10 CNV regions that exhibited higher aberration scores or larger global effects and are thus confirmable by PCR. Test PCR verified the loss of gene dosage at two loci (1p36.21 and 1p13.3) and confirmed the global variation of the copy number distributions at two loci (11p15.4 and 13q21.1), both indicating the utility of the present strategy. These test loci, however, exhibited the same somatic CNV patterns in the other brain region. 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Assessment of copy number variations in the brain genome of schizophrenia patients
http://hdl.handle.net/10191/35555
http://hdl.handle.net/10191/3555548bb575a-e6e8-45df-b537-e5e999582ff5
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要旨 (179.0 kB)
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Item type | 学位論文 / Thesis or Dissertation(1) | |||||
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公開日 | 2015-12-14 | |||||
タイトル | ||||||
タイトル | Assessment of copy number variations in the brain genome of schizophrenia patients | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Assessment of copy number variations in the brain genome of schizophrenia patients | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | CNV | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Caudate | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Genome instability | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Schizophrenia | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Somatic mutation | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_46ec | |||||
タイプ | thesis | |||||
その他のタイトル | ||||||
その他のタイトル | 統合失調症患者の脳内ゲノムにおけるコピー数変異の評価 | |||||
著者 |
Sakai, Miwako
× Sakai, Miwako |
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著者別名 | ||||||
識別子 | 50650 | |||||
識別子Scheme | WEKO | |||||
姓名 | 坂井, 美和子 | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Background: Cytogenomic mutations and chromosomal abnormality are implicated in the neuropathology of several brain diseases. Cell mosaicism of brain tissues makes their detection and validation difficult, however. In the present study, we analyzed gene dosage alterations in brain DNA of schizophrenia patients and compared those with the copy number variations (CNVs) identified in schizophrenia patients as well as with those in Asian lymphocyte DNA and attempted to obtain hints at the pathological contribution of cytogenomic instability in schizophrenia. Results: Brain DNA was extracted from postmortem striatum of schizophrenia patients and control subjects (n = 48 each) and subjected to the direct two color microarray analysis that limits technical data variations. Disease-associated biases of relative DNA doses were statistically analyzed with Bonferroni's compensation on the premise of brain cell mosaicism. We found that the relative gene dosage of 85 regions significantly varied among a million of probe sites. In the candidate CNV regions, 26 regions had no overlaps with the common CNVs found in Asian populations and included the genes (i.e., ANTXRL, CHST9, DNM3, NDST3, SDK1, STRC, SKY) that are associated with schizophrenia and/or other psychiatric diseases. The majority of these candidate CNVs exhibited high statistical probabilities but their signal differences in gene dosage were less than 1.5-fold. For test evaluation, we rather selected the other 10 CNV regions that exhibited higher aberration scores or larger global effects and are thus confirmable by PCR. Test PCR verified the loss of gene dosage at two loci (1p36.21 and 1p13.3) and confirmed the global variation of the copy number distributions at two loci (11p15.4 and 13q21.1), both indicating the utility of the present strategy. These test loci, however, exhibited the same somatic CNV patterns in the other brain region. Conclusions: The present study lists the candidate regions potentially representing cytogenomic CNVs in the brain of schizophrenia patients, although the significant but modest alterations in their brain genome doses largely remain to be characterized further. | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 学位の種類: 博士(医学). 報告番号: 甲第4063号. 学位記番号: 新大院博(医)甲第652号. 学位授与年月日: 平成27年9月24日 | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Molecular Cytogenetics. 2015, 8, 46. | |||||
書誌情報 | 発行日 2015-09-24 | |||||
出版者 | ||||||
出版者 | 新潟大学 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | info:doi/10.1186/s13039-015-0144-5 | |||||
権利 | ||||||
権利情報 | Copyright(C) 2015 Sakai et al.; licensee BioMed Central. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. | |||||
著者版フラグ | ||||||
値 | ETD | |||||
学位名 | ||||||
学位名 | 博士(医学) | |||||
学位授与機関 | ||||||
学位授与機関名 | 新潟大学 | |||||
学位授与年月日 | ||||||
学位授与年月日 | 2015-09-24 | |||||
学位授与番号 | ||||||
学位授与番号 | 13101甲第4063号 | |||||
学位記番号 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 新大院博(医)甲第652号 |