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Novel assay to detect increased level of neutralizing anti-interferon gamma autoantibodies in non-tuberculous mycobacterial patients
http://hdl.handle.net/10191/32273
http://hdl.handle.net/10191/32273e957b388-2751-4bc2-8aab-032ab7edd012
| 名前 / ファイル | ライセンス | アクション |
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本文 (618.3 kB)
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要旨 (179.5 kB)
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| Item type | 学位論文 / Thesis or Dissertation(1) | |||||
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| 公開日 | 2015-06-03 | |||||
| タイトル | ||||||
| タイトル | Novel assay to detect increased level of neutralizing anti-interferon gamma autoantibodies in non-tuberculous mycobacterial patients | |||||
| タイトル | ||||||
| タイトル | Novel assay to detect increased level of neutralizing anti-interferon gamma autoantibodies in non-tuberculous mycobacterial patients | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | anti interferon gamma autoantibodies | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | disseminated non-tuberculous mycobacterium infection | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Signal Transducers and Activator of Transcription 1 | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | phosphorylation | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | diagnosis | |||||
| 資源タイプ | ||||||
| 資源 | http://purl.org/coar/resource_type/c_46ec | |||||
| タイプ | thesis | |||||
| その他のタイトル | ||||||
| その他のタイトル | 非結核性抗酸菌症患者における抗インターフェロンγ中和自己抗体検出法 | |||||
| 著者 |
Shima, Kenjiro
× Shima, Kenjiro |
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| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 50494 | |||||
| 姓名 | 島, 賢治郎 | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Subjects exposed to non-tuberculouse mycobacterium (NTM) species do not always develop an active disease, which likely reflects underlying host susceptibility factors. Recent reports have shown that anti interferon gamma (IFN-γ) neutralizing autoantibodies (IFN-γAb) are associated with the development of disseminated NTM in patients without known evidence of immunodeficiency. The purpose of this study is to establish the screening method if subjects have IFN-γ Ab. Whole blood was obtained from patients with disseminated NTM, those with pulmonary NYM, and healthy controls. The neutralizing capacity to IFN-γ activity was assessed as an inhibition of Signal Transducer and Activation of Transcription 1 (STAT-1) phosphorylation in leukocyte after stimulation with exogenous IFN-γ by flow cytometer. The strength of phosphorylation was described as STAT1 phosphorylation index. Antigen capture assay was performed to measure the relative titer of Immunoglobulin-G fraction of IFN-γ Ab. STAT1 phosphorylation by IFN-γ was significantly inhibited in the leukocytes from patients with disseminated NTM compared to that in healthy subjects, while this inhibition was not observed in patients with pulmonary NYM. All subjects with inhibited STAT1 phosphorylation had high titer of Immunoglobulin-G that reacted with IFN-γ in the antigen capture assay. The measurement of STAT1 phosphorylation index in whole blood leukocytes and antigen capture assay are simple and useful method for detection of anti- IFN-γ neutralizing autoantibodies, and is valuable in the pathophysiological diagnosis of disseminated NTM patients without obvious immunodeficiency. | |||||
| 内容記述 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 学位の種類: 博士（医学）. 報告番号: 甲第3989号. 学位記番号: 新大院博（医）甲第635号. 学位授与年月日: 平成27年3月23日 | |||||
| 内容記述 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Journal of Infection and Chemotherapy. 2014, 20(1), 52-56. | |||||
| 書誌情報 | p. 1-24, 発行日 2015-03-23 | |||||
| 出版者 | ||||||
| 出版者 | 新潟大学 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | info:doi/10.1016/j.jiac.2013.08.003 | |||||
| 権利 | ||||||
| 権利情報 | Copyright (C) 2013 Japanese Society of Chemotherapy and The Japanese Association for Infectious Diseases. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ | |||||
| 著者版フラグ | ||||||
| 値 | ETD | |||||
| 学位名 | ||||||
| 学位名 | 博士（医学） | |||||
| 学位授与機関 | ||||||
| 学位授与機関名 | 新潟大学 | |||||
| 学位授与年月日 | ||||||
| 学位授与年月日 | 2015-03-23 | |||||
| 学位授与番号 | ||||||
| 学位授与番号 | 13101甲第3989号 | |||||
| 学位記番号 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 新大院博（医）甲第635号 | |||||
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Cite as
Shima, Kenjiro, 2015, Novel assay to detect increased level of neutralizing anti-interferon gamma autoantibodies in non-tuberculous mycobacterial patients: 新潟大学, p. 1–24.
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