ログイン
言語:

WEKO3

  • トップ
  • ランキング
To

Field does not validate

To

Field does not validate

To
lat lon distance


インデックスリンク

インデックスツリー

  • RootNode

メールアドレスを入力してください。

WEKO

One fine body…

WEKO

One fine body…

アイテム

  1. 0 資料タイプ別
  2. 02 学位論文
  1. 250 大学院医歯学総合研究科(医)
  2. 60 博士学位論文
  3. 10 博士学位論文

Real-time polymerase chain reaction analysis of MDM2 and CDK4 expression using total RNA from core-needle biopsies is useful for diagnosing adipocytic tumors

http://hdl.handle.net/10191/32272
http://hdl.handle.net/10191/32272
c507192a-018e-4814-8a68-c24de8602dc3
名前 / ファイル ライセンス アクション
h26nmk634.pdf 本文 (1.5 MB)
h26nmk634_a.pdf 要旨 (160.8 kB)
Item type 学位論文 / Thesis or Dissertation(1)
公開日 2015-06-03
タイトル
タイトル Real-time polymerase chain reaction analysis of MDM2 and CDK4 expression using total RNA from core-needle biopsies is useful for diagnosing adipocytic tumors
タイトル
タイトル Real-time polymerase chain reaction analysis of MDM2 and CDK4 expression using total RNA from core-needle biopsies is useful for diagnosing adipocytic tumors
言語 en
言語
言語 eng
キーワード
主題Scheme Other
主題 Liposarcoma
キーワード
主題Scheme Other
主題 Atypical lipomatous tumor
キーワード
主題Scheme Other
主題 Adipocytic tumors
キーワード
主題Scheme Other
主題 MDM2
キーワード
主題Scheme Other
主題 CDK4
キーワード
主題Scheme Other
主題 Real-time PCR
資源タイプ
資源 http://purl.org/coar/resource_type/c_46ec
タイプ thesis
その他のタイトル
その他のタイトル 脂肪系腫瘍に対する針生検での微量体RNAを用いたreal -time PCRによるMDM2とCDK4遺伝子の発現解析
著者 Sasaki, Taro

× Sasaki, Taro

WEKO 50491

Sasaki, Taro

Search repository
著者別名
識別子Scheme WEKO
識別子 50492
姓名 佐々木, 太郎
抄録
内容記述タイプ Abstract
内容記述 Background: Diagnosing adipocytic tumors can be challenging because it is often difficult to morphologically distinguish between benign, intermediate and malignant adipocytic tumors, and other sarcomas that are histologically similar. Recently, a number of tumor-specific chromosome translocations and associated fusion genes have been identified in adipocytic tumors and atypical lipomatous tumors/well-differentiated liposarcomas (ALT/WDL), which have a supernumerary ring and/or giant chromosome marker with amplified sequences of the MDM2 and CDK4 genes. The purpose of this study was to investigate whether quantitative real-time polymerase chain reaction (PCR) could be used to amplify MDM2 and CDK4 from total RNA samples obtained from core-needle biopsy sections for the diagnosis of ALT/WDL. Methods: A series of lipoma (n = 124) and ALT/WDL (n = 44) cases were analyzed for cytogenetic analysis and lipoma fusion genes, as well as for MDM2 and CDK4 expression by real-time PCR. Moreover, the expression of MDM2 and CDK4 in whole tissue sections was compared with that in core-needle biopsy sections of the same tumor in order to determine whether real-time PCR could be used to distinguish ALT/WDL from lipoma at the preoperative stage. Results: In whole tissue sections, the medians for MDM2 and CDK4 expression in ALT/WDL were higher than those in the lipomas (P < 0.05). Moreover, karyotype subdivisions with rings and/or giant chromosomes had higher MDM2 and CDK4 expression levels compared to karyotypes with 12q13-15 rearrangements, other abnormal karyotypes, and normal karyotypes (P < 0.05). On the other hand, MDM2 and CDK4 expression levels in core-needle biopsy sections were similar to those in whole-tissue sections (MDM2: P = 0.6, CDK4: P = 0.8, Wilcoxon signed-rank test). Conclusion: Quantitative real-time PCR of total RNA can be used to evaluate the MDM2 and CDK4 expression levels in core-needle biopsies and may be useful for distinguishing ALT/WDL from adipocytic tumors. Thus, total RNA from core-needle biopsy sections may have potential as a routine diagnostic tool for other tumors where gene overexpression is a feature of the tumor.
内容記述
内容記述タイプ Other
内容記述 学位の種類: 博士(医学). 報告番号: 甲第3988号. 学位記番号: 新大院博(医)甲第634号. 学位授与年月日: 平成27年3月23日
内容記述
内容記述タイプ Other
内容記述 BMC Cancer. 2014, 14, 468.
書誌情報 発行日 2015-03-23
出版者
出版者 新潟大学
DOI
識別子タイプ DOI
関連識別子 info:doi/10.1186/1471-2407-14-468
権利
権利情報 Copyright (C) 2014 Sasaki et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
著者版フラグ
値 ETD
学位名
学位名 博士(医学)
学位授与機関
学位授与機関名 新潟大学
学位授与年月日
学位授与年月日 2015-03-23
学位授与番号
学位授与番号 13101甲第3988号
学位記番号
内容記述タイプ Other
内容記述 新大院博(医)甲第634号
戻る
0
views
See details
Views

Versions

Ver.1 2021-03-01 08:16:14.901418
Show All versions

Share

Mendeley Twitter Facebook Print Addthis

Cite as

Sasaki, Taro, 2015, Real-time polymerase chain reaction analysis of MDM2 and CDK4 expression using total RNA from core-needle biopsies is useful for diagnosing adipocytic tumors: 新潟大学.

Loading...

エクスポート

OAI-PMH
  • OAI-PMH JPCOAR 2.0
  • OAI-PMH JPCOAR 1.0
  • OAI-PMH DublinCore
  • OAI-PMH DDI
Other Formats
  • JSON
  • BIBTEX

Confirm


Powered by WEKO3


Powered by WEKO3