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  1. 0 資料タイプ別
  2. 02 学位論文
  1. 250 大学院医歯学総合研究科(医)
  2. 60 博士学位論文
  3. 10 博士学位論文

Differentiation of Mouse induced Pluripotent Stem Cells into Alveolar Epithelial Cells In Vitro for Use In Vivo

http://hdl.handle.net/10191/32262
http://hdl.handle.net/10191/32262
7dbf1039-1074-4522-8d8b-419f164394b2
名前 / ファイル ライセンス アクション
h26nmk624.pdf 本文 (2.8 MB)
h26nmk624_a.pdf 要旨 (198.3 kB)
Item type 学位論文 / Thesis or Dissertation(1)
公開日 2015-06-03
タイトル
タイトル Differentiation of Mouse induced Pluripotent Stem Cells into Alveolar Epithelial Cells In Vitro for Use In Vivo
タイトル
言語 en
タイトル Differentiation of Mouse induced Pluripotent Stem Cells into Alveolar Epithelial Cells In Vitro for Use In Vivo
言語
言語 eng
資源タイプ
資源 http://purl.org/coar/resource_type/c_46ec
タイプ thesis
その他のタイトル
その他のタイトル 生体での使用を目的としたマウスiPS細胞における肺胞上皮細胞へのインビトロ分化誘導
著者 Zhou, Qiliang

× Zhou, Qiliang

WEKO 50471

Zhou, Qiliang

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著者別名
識別子 50472
識別子Scheme WEKO
姓名 周, 启亮
抄録
内容記述タイプ Abstract
内容記述 Alveolar epithelial cells (AECs) differentiated from induced pluripotent stem cells (iPSCs) represent new opportunities in lung tissue engineering and cell therapy. In this study, we modified a two-step protocol for embryonic stem cells that resulted in a yield of ~9% SPC^+ alveolar epithelial type II (AEC II) cells from mouse iPSCs in a 12-day period. The differentiated iPSCs showed morphological characteristics similar to those of AEC II cells. When differentiated iPSCs were seeded and cultured in a mouse decellularized lung scaffold, the cells re-formed an alveolar structure and expressed SPC or T1α protein, markers of AEC II or AEC I cells, respectively. Finally, the differentiated iPSCs were instilled intratracheally into a bleomycin (BLM)-induced mouse acute lung injury model. The transplanted cells integrated into the lung alveolar structure and expressed SPC and T1α. Significantly reduced lung inflammation and decreased collagen deposition were observed following differentiated iPSC transplantation. In conclusion, we report a simple and rapid protocol for in vitro differentiation of mouse iPSCs into AECs. Differentiated iPSCs show potential for regenerating three-dimensional alveolar lung structure and can be used to abrogate lung injury.
内容記述
内容記述タイプ Other
内容記述 学位の種類: 博士(医学). 報告番号: 甲第3978号. 学位記番号: 新大院博(医)甲第624号. 学位授与年月日: 平成27年3月23日
内容記述
内容記述タイプ Other
内容記述 Stem Cells Translational Medicine. 2014, 3(6), 675-685.
書誌情報 p. 1-29, 発行日 2015-03-23
出版者
出版者 新潟大学
DOI
識別子タイプ DOI
関連識別子 info:doi/10.5966/sctm.2013-0142
権利
権利情報 Copyright (C) 2015 AlphaMed Press
著者版フラグ
値 ETD
学位名
学位名 博士(医学)
学位授与機関
学位授与機関名 新潟大学
学位授与年月日
学位授与年月日 2015-03-23
学位授与番号
学位授与番号 13101甲第3978号
学位記番号
内容記述タイプ Other
内容記述 新大院博(医)甲第624号
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