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In this study, we modified a two-step protocol for embryonic stem cells that resulted in a yield of \uff5e9% SPC^+ alveolar epithelial type II (AEC II) cells from mouse iPSCs in a 12-day period. The differentiated iPSCs showed morphological characteristics similar to those of AEC II cells. When differentiated iPSCs were seeded and cultured in a mouse decellularized lung scaffold, the cells re-formed an alveolar structure and expressed SPC or T1\u03b1 protein, markers of AEC II or AEC I cells, respectively. Finally, the differentiated iPSCs were instilled intratracheally into a bleomycin (BLM)-induced mouse acute lung injury model. The transplanted cells integrated into the lung alveolar structure and expressed SPC and T1\u03b1. Significantly reduced lung inflammation and\u3000decreased collagen deposition were observed following differentiated iPSC transplantation. In conclusion, we report a simple and rapid protocol for in vitro differentiation of mouse iPSCs into AECs. Differentiated iPSCs show potential for regenerating three-dimensional alveolar lung structure and can be used to abrogate lung injury.", "subitem_description_type": "Abstract"}]}, "item_6_description_5": {"attribute_name": "\u5185\u5bb9\u8a18\u8ff0", "attribute_value_mlt": [{"subitem_description": "\u5b66\u4f4d\u306e\u7a2e\u985e: \u535a\u58eb\uff08\u533b\u5b66\uff09. \u5831\u544a\u756a\u53f7: \u7532\u7b2c3978\u53f7. \u5b66\u4f4d\u8a18\u756a\u53f7: \u65b0\u5927\u9662\u535a\uff08\u533b\uff09\u7532\u7b2c624\u53f7. \u5b66\u4f4d\u6388\u4e0e\u5e74\u6708\u65e5: \u5e73\u621027\u5e743\u670823\u65e5", "subitem_description_type": "Other"}, {"subitem_description": "Stem Cells Translational Medicine. 2014, 3(6), 675-685.", "subitem_description_type": "Other"}]}, "item_6_description_53": {"attribute_name": "\u5b66\u4f4d\u8a18\u756a\u53f7", "attribute_value_mlt": [{"subitem_description": "\u65b0\u5927\u9662\u535a\uff08\u533b\uff09\u7532\u7b2c624\u53f7", "subitem_description_type": "Other"}]}, "item_6_dissertation_number_52": {"attribute_name": "\u5b66\u4f4d\u6388\u4e0e\u756a\u53f7", "attribute_value_mlt": [{"subitem_dissertationnumber": "13101\u7532\u7b2c3978\u53f7"}]}, "item_6_full_name_3": {"attribute_name": "\u8457\u8005\u5225\u540d", "attribute_value_mlt": [{"nameIdentifiers": [{"nameIdentifier": "50472", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "\u5468, \u542f\u4eae"}]}]}, "item_6_publisher_7": {"attribute_name": "\u51fa\u7248\u8005", "attribute_value_mlt": [{"subitem_publisher": "\u65b0\u6f5f\u5927\u5b66"}]}, "item_6_relation_14": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "info:doi/10.5966/sctm.2013-0142", "subitem_relation_type_select": "DOI"}}]}, "item_6_rights_15": {"attribute_name": "\u6a29\u5229", "attribute_value_mlt": [{"subitem_rights": "Copyright (C) 2015 AlphaMed Press"}]}, "item_6_select_19": {"attribute_name": "\u8457\u8005\u7248\u30d5\u30e9\u30b0", "attribute_value_mlt": [{"subitem_select_item": "ETD"}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Zhou, Qiliang"}], "nameIdentifiers": [{"nameIdentifier": "50471", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-08-05"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "h26nmk624.pdf", "filesize": [{"value": "2.8 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 2800000.0, "url": {"label": "\u672c\u6587", "url": "https://niigata-u.repo.nii.ac.jp/record/5549/files/h26nmk624.pdf"}, "version_id": "d65142c4-5802-458c-a3aa-83f65fe1c0a5"}, {"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-08-05"}], "displaytype": "detail", "download_preview_message": "", "file_order": 1, "filename": "h26nmk624_a.pdf", "filesize": [{"value": "198.3 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 198300.0, "url": {"label": "\u8981\u65e8", "url": "https://niigata-u.repo.nii.ac.jp/record/5549/files/h26nmk624_a.pdf"}, "version_id": "691d8dd1-94b8-4ed7-b673-f397e39153a5"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "thesis", "resourceuri": "http://purl.org/coar/resource_type/c_46ec"}]}, "item_title": "Differentiation of Mouse induced Pluripotent Stem Cells into Alveolar Epithelial Cells In Vitro for Use In Vivo", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "Differentiation of Mouse induced Pluripotent Stem Cells into Alveolar Epithelial Cells In Vitro for Use In Vivo"}, {"subitem_title": "Differentiation of Mouse induced Pluripotent Stem Cells into Alveolar Epithelial Cells In Vitro for Use In Vivo", "subitem_title_language": "en"}]}, "item_type_id": "6", "owner": "1", "path": ["453/455", "471/561/562"], "permalink_uri": "http://hdl.handle.net/10191/32262", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_value": "2015-06-03"}, "publish_date": "2015-06-03", "publish_status": "0", "recid": "5549", "relation": {}, "relation_version_is_last": true, "title": ["Differentiation of Mouse induced Pluripotent Stem Cells into Alveolar Epithelial Cells In Vitro for Use In Vivo"], "weko_shared_id": 2}