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LC/MSシステムによる試料中アブシジン酸の同定と, 高速液体クロマトグラフィーによるダイズ種子中のアブシジン酸含有量の迅速測定
http://hdl.handle.net/10191/23754
http://hdl.handle.net/10191/237544d214493-f575-47d1-a0ba-01074898bf84
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
110001751816.pdf (585.6 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2013-10-09 | |||||
| タイトル | ||||||
| タイトル | LC/MSシステムによる試料中アブシジン酸の同定と, 高速液体クロマトグラフィーによるダイズ種子中のアブシジン酸含有量の迅速測定 | |||||
| タイトル | ||||||
| タイトル | LC/MSシステムによる試料中アブシジン酸の同定と, 高速液体クロマトグラフィーによるダイズ種子中のアブシジン酸含有量の迅速測定 | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | jpn | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | ダイズ種子 | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | アブシジン酸 | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | LC/MSシステム | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | abscisic acid | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | LC/MS system | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | soybean seed | |||||
| 資源タイプ | ||||||
| 資源 | http://purl.org/coar/resource_type/c_6501 | |||||
| タイプ | journal article | |||||
| その他のタイトル | ||||||
| その他のタイトル | Identification of Abscisic Acid by LC/MS System and Rapid Determination of the Abscisic Acid Concentration in Soybean Seeds Using High Performance Liquid Chromatography | |||||
| 著者 |
大竹, 憲邦
× 大竹, 憲邦× 山田, 真也× 大山, 卓爾 |
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| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 47395 | |||||
| 姓名 | Ohtake, Norikuni | |||||
| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 47396 | |||||
| 姓名 | Yamada, Shinya | |||||
| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 52 | |||||
| 姓名 | Ohyama, Takuji | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Abscisic acid (ABA) is one of the photohormones related to plant metabolism, development and senescence. For determination of ABA, tritium- or deuterium-labeled ABA has been used as an internal standard, because incomplete recovery was expected in the extraction and purification procedures. The simple purification method was applied for the determination of ABA concentration in soybean seeds without any internal standard. The soybean seeds were extracted with methanol including 2.6-di-tert-butyl-p-crezol as an antioxidant. After methanol was evaporated to dryness, the residue was redissolved in water, and the impurities in the solution were removed by mini column of octadecylsilane (C 18). The ABA was quantitatively recovered from the column and ABA gave a single UV peak by high performance liquid chromatography with a SIL 5-B column. The identification of ABA was done by a liquid chromatography/mass spectrometer (LC/MS) system. The mass spectrum of standard ABA and the peak with the same retention time from soybean seeds gave the similar fragmentation patterns with various voltages of ionization. Also, the values obtained by mass spectrum with 263 m/z, a major peak of ABA and those by UV absorbancy, showed the same concentrations. UV spectrum of standard ABA and isolated ABA fraction from LC showed the same curve. From these results, the ABA concentration in soybean seeds can be analyzed simply by partial purification with a C 18 mini-column and analyzed by HPLC with UV detecter without an internal standard. The LC/MS system is very useful to identify the LC peak and to detectimpurities in the ABA fraction. ABA concentration change in seeds was compared in two isogenic lines, nodulated T 202 and non-nodulated T 201, grown in a rotated paddy field of Niigata Agricultural Station during seed development. In the early stage of seeds development (September 1, 1993), the ABA concentration of T202 seeds was initially higher and then decreased, while that of T201 seeds remained high in the middle stage of seeds development (September 22, 1993). The differential changes in the ABA concentration of seeds may influence nitrogen nutrition of plants or seed storage protein accumulation. | |||||
| 書誌情報 |
日本土壌肥料學雜誌 en : 日本土壌肥料學雜誌 巻 68, 号 1, p. 52-58, 発行日 1997-02 |
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| 出版者 | ||||||
| 出版者 | 日本土壌肥料学会 | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 00290610 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AN00195767 | |||||
| 権利 | ||||||
| 権利情報 | 一般社団法人日本土壌肥料学会 | |||||
| 権利 | ||||||
| 権利情報 | 本文データは学協会の許諾に基づきCiNiiから複製したものである | |||||
| 著者版フラグ | ||||||
| 値 | publisher | |||||
| 異版である | ||||||
| 関連タイプ | isVersionOf | |||||
| 識別子タイプ | URI | |||||
| 関連識別子 | http://ci.nii.ac.jp/naid/110001751816 | |||||
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Cite as
大竹, 憲邦, 山田, 真也, 大山, 卓爾, 1997, LC/MSシステムによる試料中アブシジン酸の同定と, 高速液体クロマトグラフィーによるダイズ種子中のアブシジン酸含有量の迅速測定: 日本土壌肥料学会, 52–58 p.
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