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  1. 0 資料タイプ別
  2. 02 学位論文
  1. 250 大学院医歯学総合研究科(医)
  2. 60 博士学位論文
  3. 10 博士学位論文

Development of a non-alcoholic steatohepatitis model with rapid accumulation of fibrosis, and its treatment using mesenchymal stem cells and their small extracellular vesicles

http://hdl.handle.net/10191/00051949
http://hdl.handle.net/10191/00051949
060eac38-943d-4e43-9e89-0be779670d8c
名前 / ファイル ライセンス アクション
r2nmk958.pdf 本文 (4.1 MB)
r2nmk958_a.pdf 要旨 (456.5 kB)
Item type 学位論文 / Thesis or Dissertation(1)
公開日 2020-11-12
タイトル
タイトル Development of a non-alcoholic steatohepatitis model with rapid accumulation of fibrosis, and its treatment using mesenchymal stem cells and their small extracellular vesicles
言語
言語 eng
キーワード
主題Scheme Other
主題 Non-alcoholic steatohepatitis
キーワード
主題Scheme Other
主題 Cirrhosis
キーワード
主題Scheme Other
主題 Mesenchymal stem cells
キーワード
主題Scheme Other
主題 Small extracellular vesicles
資源タイプ
資源 http://purl.org/coar/resource_type/c_46ec
タイプ thesis
その他のタイトル
その他のタイトル 線維化を伴う非アルコール性脂肪性肝炎のモデルマウスの開発、および間葉系幹細胞とその細胞分泌小胞を投与した際の治療効果の検証
著者 Watanabe, Takayuki

× Watanabe, Takayuki

WEKO 178204

Watanabe, Takayuki

Search repository
著者別名
識別子 178205
識別子Scheme WEKO
姓名 渡邉, 貴之
抄録
内容記述タイプ Abstract
内容記述 Introduction: Currently, there are no approved drugs for treating non-alcoholic steatohepatitis (NASH); however, mesenchymal stem cells (MSCs) and their small extracellular vesicles (sEVs), which possess immunomodulatory activities, are potential candidates. This study aimed to develop a mouse model of NASH with rapid accumulation of fibrosis using the pre-established melanocortin type-4 receptor knockout (Mc4r-KO) NASH mouse model and lipopolysaccharide (LPS), and to evaluate the therapeutic effect of MSCs and their sEVs. Methods: Mc4r-KO mice (8 weeks old, male) were fed a western diet (WD) for 8 weeks. Next, the mice were intraperitoneally injected with lipopolysaccharide (LPS) twice a week for 4 weeks while continuing the WD. To confirm the therapeutic effect of MSCs and sEVs, human adipose tissue-derived MSCs or their sEVs were administered 12 weeks after initiation of the WD, and serum testing, quantitative analysis of fibrosis, and quantitative reverse transcription-polymerase chain reaction qRT-PCR were performed. Results: By providing a WD combined with LPS treatment, we successfully developed a NASH model with rapid accumulation of fibrosis. Both human MSCs and their sEVs decreased serum alanine transaminase levels and inflammatory markers based on qRT-PCR. Histological analysis showed that MSC or sEV treatment did not affect fat accumulation. However, an improvement in fibrosis in the groups treated with MSCs and their sEVs was observed. Furthermore, after administering MSCs and sEVs, there was a significant increase in anti-inflammatory macrophages in the liver. Conclusion: We successfully developed a NASH model with rapid accumulation of fibrosis and confirmed the anti-inflammatory and anti-fibrotic effects of MSCs and their sEVs, which may be options for future therapy.
内容記述
内容記述タイプ Other
内容記述 Regenerative Therapy. 2020, 14, 252-261.
DOI
識別子タイプ DOI
関連識別子 info:doi/10.1016/j.reth.2020.03.012
権利
権利情報 【○!C】 2020, The Japanese Society for Regenerative Medicine. Production and hosting by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
著者版フラグ
値 ETD
学位名
学位名 博士(医学)
学位授与機関
学位授与機関名 新潟大学
学位授与年月日
学位授与年月日 2020-09-23
学位授与番号
学位授与番号 13101甲第4788号
学位記番号
内容記述タイプ Other
内容記述 新大院博(医)甲第958号
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