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Significance of a histone-like protein with its native structure for the diagnosis of asymptomatic tuberculosis
http://hdl.handle.net/10191/00051842
http://hdl.handle.net/10191/00051842cdeab670-a010-47f8-8b36-2c4a945efaaa
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本文 (1.5 MB)
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要旨 (570.9 kB)
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| Item type | 学位論文 / Thesis or Dissertation(1) | |||||
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| 公開日 | 2020-08-31 | |||||
| タイトル | ||||||
| タイトル | Significance of a histone-like protein with its native structure for the diagnosis of asymptomatic tuberculosis | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源 | http://purl.org/coar/resource_type/c_46ec | |||||
| タイプ | thesis | |||||
| その他のタイトル | ||||||
| その他のタイトル | 無症候性結核の診断における天然変性ヒストン様蛋白質の生来構造の重要性 | |||||
| 著者 |
Ohara, Yukiko
× Ohara, Yukiko |
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| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 178087 | |||||
| 姓名 | 大原, 由貴子 | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Tuberculosis causes the highest mortality among all single infections. Asymptomatic tuberculosis, afflicting one third of the global human population, is the major source as 5–10% of asymptomatic cases develop active tuberculosis during their lifetime. Thus it is one of important issues to develop diagnostic tools for accurately detecting asymptomatic infection. Mycobacterial DNA-binding protein 1 (MDP1) is a major protein in persistent Mycobacterium tuberculosis and has potential for diagnostic use in detecting asymptomatic infection. However, a previous ELISA-based study revealed a specificity problem; IgGs against MDP1were detected in both M. tuberculosis-infected and uninfected individuals. Although the tertiary structures of an antigen are known to influence antibody recognition, the MDP1 structural details have not yet been investigated. The N-terminal half of MDP1, homologous to bacterial histone-like protein HU, is predicted to be responsible for DNA-binding, while the C-terminal half is assumed as totally intrinsically disordered regions. To clarify the relationship between the MDP1 tertiary structure and IgG recognition, we refined the purification method, which allow us to obtain a recombinant protein with the predicted structure. Furthermore, we showed that an IgG-ELISA using MDP1 purified by our refined method is indeed useful in the detection of asymptomatic tuberculosis. | |||||
| 内容記述 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | PLoS One. 2018, 13(10), e0204160. | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | info:doi/10.1371/journal.pone.0204160 | |||||
| 権利 | ||||||
| 権利情報 | 【○!C】 2018 Ohara et al. This is an open access article distributed under the terms of the Creative Commons Attribution License. | |||||
| 著者版フラグ | ||||||
| 値 | ETD | |||||
| 学位名 | ||||||
| 学位名 | 博士(医学) | |||||
| 学位授与機関 | ||||||
| 学位授与機関名 | 新潟大学 | |||||
| 学位授与年月日 | ||||||
| 学位授与年月日 | 2020-03-23 | |||||
| 学位授与番号 | ||||||
| 学位授与番号 | 13101甲第4719号 | |||||
| 学位記番号 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 新大院博(医)甲第953号 | |||||
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Ohara, Yukiko, n.d., Significance of a histone-like protein with its native structure for the diagnosis of asymptomatic tuberculosis.
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