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MGMT Expression Contributes to Temozolomide Resistance in H3K27M-Mutant Diffuse Midline Gliomas
http://hdl.handle.net/10191/00051798
http://hdl.handle.net/10191/000517984dfcae91-9c1f-443a-9d8f-82e57edbb020
名前 / ファイル | ライセンス | アクション |
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要旨 (575.9 kB)
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Item type | 学位論文 / Thesis or Dissertation(1) | |||||
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公開日 | 2020-08-31 | |||||
タイトル | ||||||
タイトル | MGMT Expression Contributes to Temozolomide Resistance in H3K27M-Mutant Diffuse Midline Gliomas | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | MGMT | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | diffuse midline gliomas | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | histone H3 mutation | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | epigenetics | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | DNA hypomethylation | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_46ec | |||||
タイプ | thesis | |||||
その他のタイトル | ||||||
その他のタイトル | H3K27M変異型びまん性正中グリオーマにおけるMGMT発現はテモゾロマイド抵抗性に寄与する | |||||
著者 |
Abe, Hideaki
× Abe, Hideaki |
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著者別名 | ||||||
識別子 | 178000 | |||||
識別子Scheme | WEKO | |||||
姓名 | 阿部, 英明 | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Diffuse midline gliomas (DMGs) show resistance to many chemotherapeutic agents including temozolomide (TMZ). Histone gene mutations in DMGs trigger epigenetic changes including DNA hypomethylation, one of which is a frequent lack ofO6-methyl-guanine-DNA methyltransferase (MGMT) promoter methylation, resulting in increased MGMT expression. We established the NGT16 cell line with HIST1H3B K27Mand ACVR1 G328E gene mutations from a DMG patient and used this cell line and other DMG cell lines with H3F3A gene mutation (SF7761, SF8628, JHH-DIPG1) to analyze MGMT promoter methylation, MGMT protein expression, and response to TMZ. Three out of 4 DMG cell lines (NGT16, SF8628, and JHH-DIPG1) had unmethylated MGMT promoter, increased MGMT expression, and showed resistance to TMZ treatment. SF7761 cells with H3F3A gene mutation showed MGMT promoter methylation, lacked MGMT expression, and sensitivity to TMZ treatment. NGT16 line showed response toALK2 inhibitor K02288 treatment in vitro. We confirmed in vitro that MGMT expression contributes to TMZ resistance in DMG cell lines. There is an urgent need to develop new strategies to treat TMZ-resistant DMGs. | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Neurologia medico-chirurgica(Tokyo). 2018, 58(7), 290-295. | |||||
書誌情報 | p. 1-10 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | info:doi/10.2176/nmc.ra.2018-0044 | |||||
権利 | ||||||
権利情報 | 【○!C】 2020 Abe, Natsumeda, Okada, Watanabe, Tsukamoto, Kanemaru, Yoshimura, Oishi, Hashizume, Kakita and Fujii. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). | |||||
著者版フラグ | ||||||
値 | ETD | |||||
学位名 | ||||||
学位名 | 博士(医学) | |||||
学位授与機関 | ||||||
学位授与機関名 | 新潟大学 | |||||
学位授与年月日 | ||||||
学位授与年月日 | 2020-03-23 | |||||
学位授与番号 | ||||||
学位授与番号 | 13101甲第4675号 | |||||
学位記番号 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 新大院博(医)甲第909号 |