WEKO3
アイテム
Transcriptomic Dissection of Hepatocyte Heterogeneity : Linking Ploidy, Zonation, and Stem/Progenitor Cell Characteristics
http://hdl.handle.net/10191/00051796
http://hdl.handle.net/10191/00051796800c2839-49f3-46e6-9868-b13ed68e7a25
名前 / ファイル | ライセンス | アクション |
---|---|---|
本文 (11.1 MB)
|
|
|
要旨 (570.8 kB)
|
|
Item type | 学位論文 / Thesis or Dissertation(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2020-08-31 | |||||
タイトル | ||||||
タイトル | Transcriptomic Dissection of Hepatocyte Heterogeneity : Linking Ploidy, Zonation, and Stem/Progenitor Cell Characteristics | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Hepatocyte | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Ploidy | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Zonation | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Single-Cell PCR | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Transcriptome | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_46ec | |||||
タイプ | thesis | |||||
その他のタイトル | ||||||
その他のタイトル | トランスクリプトーム解析が示す肝細胞不均一性 : 倍数性、肝内分布、幹/前駆細胞の観点から | |||||
著者 |
Hosaka, Kazunori
× Hosaka, Kazunori |
|||||
著者別名 | ||||||
識別子 | 177996 | |||||
識別子Scheme | WEKO | |||||
姓名 | 保坂, 和徳 | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | By using rat hepatocytes with different ploidy statuses, a bulk transcriptome and single-cell quantitative reverse-transcription polymerase chain reaction show that diploid hepatocytes are preferentially located in the pericentral region. Single-cell analysis further identifies a subpopulation within the 2c hepatocytes that co-express the mature hepatocyte markers and liver progenitor cell markers. BACKGROUND & AIMS: There is a long-standing debate regarding the biological significance of polyploidy in hepatocytes. Recent studies have provided increasing evidence that hepatocytes with different ploidy statuses behave differently in a context-dependent manner (eg, susceptibility to oncogenesis, regenerative ability after injury, and in vitro proliferative capacity). However, their overall transcriptomic differences in a physiological context is not known. METHODS: By using microarray transcriptome analysis, we investigated the heterogeneity of hepatocyte populations with different ploidy statuses. Moreover, by using single-cell quantitative reverse-transcription polymerase chain reaction (scPCR) analysis, we investigated the intrapopulational transcriptome heterogeneity of 2c and 4c hepatocytes. RESULTS: Microarray analysis showed that cell cycle–related genes were enriched in 8c hepatocytes, which is in line with the established notion that polyploidy is formed via cell division failure. Surprisingly, in contrast to the general consensus that 2c hepatocytes reside in the periportal region, in our bulk transcriptome and scPCR analyses, the 2c hepatocytes consistently showed pericentral hepatocyte-enriched characteristics. In addition, scPCR analysis identified a subpopulation within the 2c hepatocytes that co-express the liver progenitor cell markers Axin2, Prom1, and Lgr5, implying the potential biological relevance of this subpopulation. CONCLUSIONS: This study provides new insights into hepatocyte heterogeneity, namely 2c hepatocytes are preferentially localized to the pericentral region, and a subpopulation of 2c hepatocytes show liver progenitor cell–like features in terms of liver progenitor cell marker expression (Axin2, Prom1, andLgr5). | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Cellular and Molecular Gastroenterology and Hepatology. 2020, 9(1), 161-183. | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | info:doi/10.1016/j.jcmgh.2019.08.011 | |||||
権利 | ||||||
権利情報 | 【○!C】 2020 The Authors. Published by Elsevier Inc. on behalf of the AGA Institute. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). | |||||
著者版フラグ | ||||||
値 | ETD | |||||
学位名 | ||||||
学位名 | 博士(医学) | |||||
学位授与機関 | ||||||
学位授与機関名 | 新潟大学 | |||||
学位授与年月日 | ||||||
学位授与年月日 | 2020-03-23 | |||||
学位授与番号 | ||||||
学位授与番号 | 13101甲第4673号 | |||||
学位記番号 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 新大院博(医)甲第907号 |