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  1. 0 資料タイプ別
  2. 02 学位論文
  1. 250 大学院医歯学総合研究科(医)
  2. 60 博士学位論文
  3. 10 博士学位論文

Transcriptomic Dissection of Hepatocyte Heterogeneity : Linking Ploidy, Zonation, and Stem/Progenitor Cell Characteristics

http://hdl.handle.net/10191/00051796
http://hdl.handle.net/10191/00051796
800c2839-49f3-46e6-9868-b13ed68e7a25
名前 / ファイル ライセンス アクション
r1nmk907.pdf 本文 (11.1 MB)
r1nmk907_a.pdf 要旨 (570.8 kB)
Item type 学位論文 / Thesis or Dissertation(1)
公開日 2020-08-31
タイトル
タイトル Transcriptomic Dissection of Hepatocyte Heterogeneity : Linking Ploidy, Zonation, and Stem/Progenitor Cell Characteristics
言語
言語 eng
キーワード
主題Scheme Other
主題 Hepatocyte
キーワード
主題Scheme Other
主題 Ploidy
キーワード
主題Scheme Other
主題 Zonation
キーワード
主題Scheme Other
主題 Single-Cell PCR
キーワード
主題Scheme Other
主題 Transcriptome
資源タイプ
資源 http://purl.org/coar/resource_type/c_46ec
タイプ thesis
その他のタイトル
その他のタイトル トランスクリプトーム解析が示す肝細胞不均一性 : 倍数性、肝内分布、幹/前駆細胞の観点から
著者 Hosaka, Kazunori

× Hosaka, Kazunori

WEKO 177995

Hosaka, Kazunori

Search repository
著者別名
識別子 177996
識別子Scheme WEKO
姓名 保坂, 和徳
抄録
内容記述タイプ Abstract
内容記述 By using rat hepatocytes with different ploidy statuses, a bulk transcriptome and single-cell quantitative reverse-transcription polymerase chain reaction show that diploid hepatocytes are preferentially located in the pericentral region. Single-cell analysis further identifies a subpopulation within the 2c hepatocytes that co-express the mature hepatocyte markers and liver progenitor cell markers. BACKGROUND & AIMS: There is a long-standing debate regarding the biological significance of polyploidy in hepatocytes. Recent studies have provided increasing evidence that hepatocytes with different ploidy statuses behave differently in a context-dependent manner (eg, susceptibility to oncogenesis, regenerative ability after injury, and in vitro proliferative capacity). However, their overall transcriptomic differences in a physiological context is not known. METHODS: By using microarray transcriptome analysis, we investigated the heterogeneity of hepatocyte populations with different ploidy statuses. Moreover, by using single-cell quantitative reverse-transcription polymerase chain reaction (scPCR) analysis, we investigated the intrapopulational transcriptome heterogeneity of 2c and 4c hepatocytes. RESULTS: Microarray analysis showed that cell cycle–related genes were enriched in 8c hepatocytes, which is in line with the established notion that polyploidy is formed via cell division failure. Surprisingly, in contrast to the general consensus that 2c hepatocytes reside in the periportal region, in our bulk transcriptome and scPCR analyses, the 2c hepatocytes consistently showed pericentral hepatocyte-enriched characteristics. In addition, scPCR analysis identified a subpopulation within the 2c hepatocytes that co-express the liver progenitor cell markers Axin2, Prom1, and Lgr5, implying the potential biological relevance of this subpopulation. CONCLUSIONS: This study provides new insights into hepatocyte heterogeneity, namely 2c hepatocytes are preferentially localized to the pericentral region, and a subpopulation of 2c hepatocytes show liver progenitor cell–like features in terms of liver progenitor cell marker expression (Axin2, Prom1, andLgr5).
内容記述
内容記述タイプ Other
内容記述 Cellular and Molecular Gastroenterology and Hepatology. 2020, 9(1), 161-183.
DOI
識別子タイプ DOI
関連識別子 info:doi/10.1016/j.jcmgh.2019.08.011
権利
権利情報 【○!C】 2020 The Authors. Published by Elsevier Inc. on behalf of the AGA Institute. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
著者版フラグ
値 ETD
学位名
学位名 博士(医学)
学位授与機関
学位授与機関名 新潟大学
学位授与年月日
学位授与年月日 2020-03-23
学位授与番号
学位授与番号 13101甲第4673号
学位記番号
内容記述タイプ Other
内容記述 新大院博(医)甲第907号
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