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Female gametophyte-specific gene induction system : Development and application to analyses of female gametogenesis in Arabidopsis thaliana
http://hdl.handle.net/10191/00051772
http://hdl.handle.net/10191/000517726117131a-4898-4ef2-8ed7-be2acbb07985
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本文 (2.1 MB)
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要旨 (114.8 kB)
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| Item type | 学位論文 / Thesis or Dissertation(1) | |||||
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| 公開日 | 2020-08-31 | |||||
| タイトル | ||||||
| タイトル | Female gametophyte-specific gene induction system : Development and application to analyses of female gametogenesis in Arabidopsis thaliana | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_46ec | |||||
| 資源タイプ | thesis | |||||
| その他のタイトル | ||||||
| その他のタイトル | 雌性配偶体特異的遺伝子発現誘導系 : 開発とシロイヌナズナ雌性配偶体形成過程の解析への応用 | |||||
| 著者 |
Hwang, Dukhyun
× Hwang, Dukhyun |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Flowering plants grow through somatic cell division, and leave their offspring through reproduction, which is an important lifetime. Plant reproduction involves gametophyte formation, fertilization, and embryogenesis. Female gametophyte (FG) is crucial for reproduction in flowering plants. Mutational analyses using Arabidopsis thaliana have identified genes and proteins involved in FG development. Techniques that induce gene expression by different external factors have been used as powerful tools for the analyses of various gene functions in plants. In this study, I developed a new method to analyze gene functions during female gametophyte development using heat-inducible Cre/loxP recombination system and FG-specific EMBRYO SAC 2 (ES2) promoter. Gene expression from the ES2 promoter can be detected from the four-nucleated FG stage. Efficient gene induction can be achieved by mild heat treatment at 35°C for 5 min. I applied this method to study gene functions in the fusion of polar nuclei during FG development. Sad1/UNC84 (SUN) proteins are conserved inner nuclear membrane proteins, which interact with the Klarsicht/ANC-1/Syne homology (KASH) proteins spanning the outer nuclear membrane. A. thaliana has five SUN protein genes. I used this system to express a dominant-negative mutant of SUN proteins (SUNDN) in developing FGs. Expression of SUNDN resulted in inhibition of the polar nuclear fusion, while expression of a SUNDN mutant, which does not interact with KASH proteins, did not cause the polar nuclear fusion defect. These results suggest roles of SUN-KASH interactions in the fusion of polar nuclei. The heat-inducible gene expression system developed in this study provides a new strategy for analyzing gene functions in FG development and FG functions. | |||||
| 書誌情報 | p. 1-45 | |||||
| 著者版フラグ | ||||||
| 値 | ETD | |||||
| 学位名 | ||||||
| 学位名 | 博士(理学) | |||||
| 学位授与機関 | ||||||
| 学位授与機関名 | 新潟大学 | |||||
| 学位授与年月日 | ||||||
| 学位授与年月日 | 2020-03-23 | |||||
| 学位授与番号 | ||||||
| 学位授与番号 | 13101甲第4761号 | |||||
| 学位記番号 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 新大院博(理)甲第451号 | |||||
