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Characterisation of N-glycans in the epithelial-like tissue of the rat cochlea
http://hdl.handle.net/10191/51310
http://hdl.handle.net/10191/513109470a173-c793-4b21-af68-cb7299ec3dc1
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| Item type | 学位論文 / Thesis or Dissertation(1) | |||||
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| 公開日 | 2019-07-09 | |||||
| タイトル | ||||||
| タイトル | Characterisation of N-glycans in the epithelial-like tissue of the rat cochlea | |||||
| タイトル | ||||||
| タイトル | Characterisation of N-glycans in the epithelial-like tissue of the rat cochlea | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源 | http://purl.org/coar/resource_type/c_46ec | |||||
| タイプ | thesis | |||||
| その他のタイトル | ||||||
| その他のタイトル | ラット蝸牛の上皮様組織に発現するN-結合型糖鎖の解析 | |||||
| 著者 |
Nonomura, Yoriko
× Nonomura, Yoriko |
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| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 175193 | |||||
| 姓名 | 野々村, 頼子 | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Membrane proteins (such as ion channels, transporters, and receptors) and secreted proteins are essential for cellular activities. N-linked glycosylation is involved in stability and function of these proteins and occurs at Asn residues. In several organs, profiles of N-glycans have been determined by comprehensive analyses. Nevertheless, the cochlea of the mammalian inner ear, a tiny organ mediating hearing, has yet to be examined. Here, we focused on the stria vascularis, an epithelial-like tissue in the cochlea, and characterised N-glycans by liquid chromatography with mass spectrometry. This hypervascular tissue not only expresses several ion transporters and channels to control the electrochemical balance in the cochlea but also harbours different transporters and receptors that maintain structure and activity of the organ. Seventy-nine N-linked glycans were identified in the rat stria vascularis. Among these, in 55 glycans, the complete structures were determined; in the other 24 species, partial glycosidic linkage patterns and full profiles of the monosaccharide composition were identified. In the process of characterisation, several sialylated glycans were subjected sequentially to two different alkylamidation reactions; this derivatisation helped to distinguish α2,3-linkage and α2,6-linkage sialyl isomers with mass spectrometry. These data should accelerate elucidation of the molecular architecture of the cochlea. | |||||
| 内容記述 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 学位の種類: 博士（医学）. 報告番号: 甲第4552号. 学位記番号: 新大院博（医）甲第874号. 学位授与年月日: 平成31年3月25日 | |||||
| 書誌情報 | 発行日 2019-03-25 | |||||
| 出版者 | ||||||
| 出版者 | 新潟大学 | |||||
| 著者版フラグ | ||||||
| 値 | ETD | |||||
| 学位名 | ||||||
| 学位名 | 博士（医学） | |||||
| 学位授与機関 | ||||||
| 学位授与機関名 | 新潟大学 | |||||
| 学位授与年月日 | ||||||
| 学位授与年月日 | 2019-03-25 | |||||
| 学位授与番号 | ||||||
| 学位授与番号 | 13101甲第4552号 | |||||
| 学位記番号 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 新大院博（医）甲第874号 | |||||
