{"_buckets": {"deposit": "6b051482-65d0-4603-ae97-eed1f8cfea0f"}, "_deposit": {"id": "28563", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "28563"}, "status": "published"}, "_oai": {"id": "oai:niigata-u.repo.nii.ac.jp:00028563"}, "item_7_alternative_title_1": {"attribute_name": "\u305d\u306e\u4ed6\u306e\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_alternative_title": "Direct cytotoxicity of leukemic plasmacytoid dendritic cell line\uff08PMDC05\uff09 stimulated with OK432 and IFN-\u03b3."}]}, "item_7_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2015-09", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "1", "bibliographicPageEnd": "82", "bibliographicPageStart": "77", "bibliographicVolumeNumber": "12", "bibliographic_titles": [{"bibliographic_title": "\u65b0\u6f5f\u5927\u5b66\u4fdd\u5065\u5b66\u96d1\u8a8c"}, {"bibliographic_title": "\u65b0\u6f5f\u5927\u5b66\u4fdd\u5065\u5b66\u96d1\u8a8c", "bibliographic_titleLang": "en"}]}]}, "item_7_description_4": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "\u6297\u539f\u30da\u30d7\u30c1\u30c9\u304c\u540c\u5b9a\u3055\u308c\u3066\u3044\u306a\u3044\u816b\u760d\u306b\u5bfe\u3059\u308b\u7d30\u80de\u50b7\u5bb3\u6027T\u7d30\u80de(CTL)\u3092\u8a98\u5c0e\u3059\u308b\u305f\u3081\u306b\uff0c\u5f53\u7814\u7a76\u5ba4\u3067\u6a39\u7acb\u3057\u305f\u767d\u8840\u75c5\u6027\u5f62\u8cea\u7d30\u80de\u69d8\u6a39\u72b6\u7d30\u80de\u682a(leukemic plasmacytoid dendritic cell line; PMDC05)\u306e\u76f4\u63a5\u7684\u306a\u7d30\u80de\u50b7\u5bb3\u6d3b\u6027\u3092\u691c\u51fa\u3059\u308b\u3053\u3068\u3092\u76ee\u7684\u3068\u3057\u305f\u3002\u30da\u30cb\u30b7\u30ea\u30f3\u51cd\u7d50\u4e7e\u71e5\u51e6\u7406\u3057\u305fStreptococcus pyogenes\u306e\u83cc\u4f53\u6210\u5206(OK432)\u53ca\u3073IFN-\u03b3\u3092\u7528\u3044\u3066PMDC05\u306e\u523a\u6fc0\u57f9\u990a\u3092\u884c\u3063\u305f\u3002OK432\u53ca\u3073IFN-\u03b3\u3067\u523a\u6fc0\u3055\u308c\u305fPMDC05\u306f\uff0c\u523a\u6fc0\u306a\u3057\u306b\u6bd4\u3079\u3066\u30b5\u30a4\u30c8\u30ab\u30a4\u30f3\u7523\u751f\u304c\u5897\u5f37\u3057\uff0c\u6a19\u7684\u7d30\u80de\u3092\u6709\u610f\u306b\u50b7\u5bb3\u3057\u305f\u3002\u4ee5\u4e0a\u306e\u3053\u3068\u304b\u3089\uff0cOK432\u53ca\u3073IFN-\u03b3\u3067\u523a\u6fc0\u3055\u308c\u305fPMDC05\u306f\uff0c\u816b\u760d\u7d30\u80de\u3068\u306e\u5171\u57f9\u990a\u306b\u3088\u3063\u3066\u816b\u760d\u6297\u539f\u3092\u63d0\u793a\u3057\uff0c\u816b\u760d\u6297\u539f\u7279\u7570\u7684CTL\u3092\u8a98\u5c0e\u3059\u308b\u305f\u3081\u306e\u6709\u529b\u306a\u6297\u539f\u63d0\u793a\u7d30\u80de\u306b\u306a\u308a\u5f97\u308b\u3068\u8003\u3048\u3089\u308c\u305f\u3002", "subitem_description_type": "Abstract"}, {"subitem_description": "A leukemic plasmacytoid dendritic cell line, PMDC05, which was previously established in our laboratory, showed the ability of inducing antigen specific cytotoxic T lymphocytes. For tumors that antigenic peptides are not identified, it would be necessary for PMDC05 to have direct cytotoxicity against tumor cells to uptake tumor antigens for presenting them to T cells. Therefore, we aimed to investigate the effects of OK432, penicillin-inactivated and lyophilized preparation of Streptococcus pyogenes which has been clinically used in Japan for more than 30 years, to activate PMDC05 cells. Stimulation of PMDC05 cells\u3000with OK432 and IFN-\u03b3enhanced the expression of CCR7 and TNF-related apoptosisinducing ligand (TRAIL). Also, we detected a higher cytokine production such as IL-6 and TNF-\u03b1 compared with unstimulated PMDC05 cells. Furthermore, PMDC05 cells stimulated with OK432 and IFN-\u03b3showed direct cytotoxicity against the tumor cell targets (T2A24 cells) expressing death-inducing receptors (DR4 and DR5). This might have occurred due to binding of TRAIL expressed on PMDC 05 cells with DR4/5 on T2A24 cells. These data suggest that by stimulation with OK432 and IFN-\u03b3PMDC05 cells could acquire direct antitumor cytotoxicity, which may be beneficial for PMDC05 cells to uptake tumor cell components for antigen presentation in case of PMDC05 cells are co-cultured with tumor cells. OK432/IFN-\u03b3-stimulated PMDC05 cells, which are loaded with tumor antigen by co-culturing with tumor cells, could be used as potent antigen presenting cells for generating antigen specific cytotoxic T lymphocytes (CTLs) in antitumor cellular immunotherapy.", "subitem_description_type": "Abstract"}]}, "item_7_full_name_3": {"attribute_name": "\u8457\u8005\u5225\u540d", "attribute_value_mlt": [{"nameIdentifiers": [{"nameIdentifier": "164407", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "Oiwa, Eri"}]}, {"nameIdentifiers": [{"nameIdentifier": "164408", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "Narita, Miwako"}]}, {"nameIdentifiers": [{"nameIdentifier": "164409", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "Iwaya, Shunpei"}]}, {"nameIdentifiers": [{"nameIdentifier": "164410", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "Nishizawa, Yoshinori"}]}, {"nameIdentifiers": [{"nameIdentifier": "164411", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "Uchiyama, Takayoshi"}]}, {"nameIdentifiers": [{"nameIdentifier": "164412", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "Takahashi, Masuhiro"}]}]}, "item_7_publisher_7": {"attribute_name": "\u51fa\u7248\u8005", "attribute_value_mlt": [{"subitem_publisher": "\u65b0\u6f5f\u5927\u5b66\u533b\u5b66\u90e8\u4fdd\u5065\u5b66\u79d1"}]}, "item_7_select_19": {"attribute_name": "\u8457\u8005\u7248\u30d5\u30e9\u30b0", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_7_source_id_11": {"attribute_name": "\u66f8\u8a8c\u30ec\u30b3\u30fc\u30c9ID", "attribute_value_mlt": [{"subitem_source_identifier": "AA12680484", "subitem_source_identifier_type": "NCID"}]}, "item_7_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "21884617", "subitem_source_identifier_type": "ISSN"}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "\u5927\u5ca9, \u6075\u7406"}], "nameIdentifiers": [{"nameIdentifier": "164401", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "\u6210\u7530, \u7f8e\u548c\u5b50"}], "nameIdentifiers": [{"nameIdentifier": "164402", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "\u5ca9\u8c37, \u4fca\u5e73"}], "nameIdentifiers": [{"nameIdentifier": "164403", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "\u897f\u6fa4, \u5e79\u5247"}], "nameIdentifiers": [{"nameIdentifier": "164404", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "\u5185\u5c71, \u5b5d\u7531"}], "nameIdentifiers": [{"nameIdentifier": "164405", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "\u9ad8\u6a4b, \u76ca\u5ee3"}], "nameIdentifiers": [{"nameIdentifier": "164406", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-08-20"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "12(1)_77-82.pdf", "filesize": [{"value": "315.9 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 315900.0, "url": {"label": "12(1)_77-82.pdf", "url": "https://niigata-u.repo.nii.ac.jp/record/28563/files/12(1)_77-82.pdf"}, "version_id": "d455d8ef-fdc1-4268-8013-10e373b44c22"}]}, "item_keyword": {"attribute_name": "\u30ad\u30fc\u30ef\u30fc\u30c9", "attribute_value_mlt": [{"subitem_subject": "leukemic plasmacytoid dendritic cell line; PMDC05", "subitem_subject_scheme": "Other"}, {"subitem_subject": "OK432", "subitem_subject_scheme": "Other"}, {"subitem_subject": "IFN-\u03b3", "subitem_subject_scheme": "Other"}, {"subitem_subject": "\u7d30\u80de\u50b7\u5bb3\u6d3b\u6027", "subitem_subject_scheme": "Other"}, {"subitem_subject": "\u6297\u816b\u760d\u514d\u75ab", "subitem_subject_scheme": "Other"}, {"subitem_subject": "IFN-\u03b3cytotoxicity", "subitem_subject_scheme": "Other"}, {"subitem_subject": "anti-tumor immunity", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "jpn"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "OK432\u53ca\u3073IFN-\u03b3\u523a\u6fc0\u306b\u3088\u308b\u767d\u8840\u75c5\u6027\u5f62\u8cea\u7d30\u80de\u69d8\u6a39\u72b6\u7d30\u80de\u682a\uff08PMDC05\uff09\u306e\u76f4\u63a5\u7d30\u80de\u50b7\u5bb3\u6d3b\u6027\u306e\u691c\u51fa", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "OK432\u53ca\u3073IFN-\u03b3\u523a\u6fc0\u306b\u3088\u308b\u767d\u8840\u75c5\u6027\u5f62\u8cea\u7d30\u80de\u69d8\u6a39\u72b6\u7d30\u80de\u682a\uff08PMDC05\uff09\u306e\u76f4\u63a5\u7d30\u80de\u50b7\u5bb3\u6d3b\u6027\u306e\u691c\u51fa"}, {"subitem_title": "OK432\u53ca\u3073IFN-\u03b3\u523a\u6fc0\u306b\u3088\u308b\u767d\u8840\u75c5\u6027\u5f62\u8cea\u7d30\u80de\u69d8\u6a39\u72b6\u7d30\u80de\u682a\uff08PMDC05\uff09\u306e\u76f4\u63a5\u7d30\u80de\u50b7\u5bb3\u6d3b\u6027\u306e\u691c\u51fa", "subitem_title_language": "en"}]}, "item_type_id": "7", "owner": "1", "path": ["453/456", "506/1624/1625/1629"], "permalink_uri": "http://hdl.handle.net/10191/38959", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_value": "2016-03-01"}, "publish_date": "2016-03-01", "publish_status": "0", "recid": "28563", "relation": {}, "relation_version_is_last": true, "title": ["OK432\u53ca\u3073IFN-\u03b3\u523a\u6fc0\u306b\u3088\u308b\u767d\u8840\u75c5\u6027\u5f62\u8cea\u7d30\u80de\u69d8\u6a39\u72b6\u7d30\u80de\u682a\uff08PMDC05\uff09\u306e\u76f4\u63a5\u7d30\u80de\u50b7\u5bb3\u6d3b\u6027\u306e\u691c\u51fa"], "weko_shared_id": null}