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  1. 0 資料タイプ別
  2. 03 紀要論文
  1. 070 農学部
  2. 20 紀要
  3. 01 新潟大学農学部研究報告
  4. 第55巻第2号

Histochemical Studies on progestagen Production in Mouse Eggs and Embryos during the Early Development

http://hdl.handle.net/10191/25112
http://hdl.handle.net/10191/25112
f540d1ad-6d22-4317-b599-e8cef2a8dfc3
名前 / ファイル ライセンス アクション
55_2_91-97.pdf 55_2_91-97.pdf (490.1 kB)
Item type 紀要論文 / Departmental Bulletin Paper(1)
公開日 2014-02-12
タイトル
タイトル Histochemical Studies on progestagen Production in Mouse Eggs and Embryos during the Early Development
タイトル
言語 en
タイトル Histochemical Studies on progestagen Production in Mouse Eggs and Embryos during the Early Development
言語
言語 eng
キーワード
主題Scheme Other
主題 mouse embryo
キーワード
主題Scheme Other
主題 early development
キーワード
主題Scheme Other
主題 hydroxysteroid dehydrogenase
キーワード
主題Scheme Other
主題 progestagen production
キーワード
主題Scheme Other
主題 histochemistry
キーワード
主題Scheme Other
主題 マウス胚
キーワード
主題Scheme Other
主題 初期発生
キーワード
主題Scheme Other
主題 ヒドロキシステロイド脱水素酵素
キーワード
主題Scheme Other
主題 プロジェスタジェン合成
キーワード
主題Scheme Other
主題 組織化学
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ departmental bulletin paper
その他のタイトル
その他のタイトル 発生初期のマウスの卵子と初期胚におけるプロジェスタジェン合成に関する組織化学的研究
著者 Niimura, Sueo

× Niimura, Sueo

WEKO 163933

Niimura, Sueo

Search repository
Kawakami, Shin-ya

× Kawakami, Shin-ya

WEKO 163934

Kawakami, Shin-ya

Search repository
著者別名
識別子 163935
識別子Scheme WEKO
姓名 新村, 末雄
著者別名
識別子 163936
識別子Scheme WEKO
姓名 川上, 心也
抄録
内容記述タイプ Abstract
内容記述 The activities of △^5-3β-hydroxysteroid dehydrogenase (△^5-3β-HSD) (pregnenolone and 17α-hydroxypregnenolone as the substrates), 20α-HSD (20α-hydroxyprogesterone as the substrate) and 20β-HSD (17α-hydroxyprogesterone and 20β-hydroxyprogesterone as the substrates) were histochemically demonstrated in mouse eggs and embryos, and the progestagen production in those during the early development was examined. In unfertilized mouse eggs, the activities of such HSDs were observed in 88 to 98% of those. The percentages of embryos showing the activities of such HSDs did not change during the early development. It was also histochemically confirmed that the activity of △^5-3β HSD with DHA as the substrate, which is involved in steroid biosynthesis, was observed in 86 to 100% of the eggs and embryos from the un fertilized to blastocyst stages. From the present findings, it was suggested that the progestagens such as progesterone, 17α-hydroxyprogesterone, 17α,20β-dihydroxyprogesterone, 20α-hydroxyprogesterone and 20β-hydroxyprogesterone are constantly synthesized in mouse eggs and embryos during the early development.
抄録
内容記述タイプ Abstract
内容記述 マウスの未受精卵子および前核期から胚盤抱期までの初期胚について、△^5-3β-HSD(基質としてpregnenoloneおよび17α-hydroxypregnenoloneを使用)、 20α-HSD (20α-hydroxyprogesterone)および20β-HSD (17α-hydroxyprogesterone、 20β-hydroxyprogesterone)の活性を組織化学的に検出し、プロジェスタジェン合成能を検肘した。未受精期の卵子において、これらHSDの活性は88ないし98%のものに認められた。また、前核期から胚盤抱期までの初期胚においても、 83%以上でこれらのHSD活性が検出された。一方、ステロイド生合成の指標となる△^5-3β-HSD (DHA)の活性は、 86%以上の卵子と初期胚に常に観察された。これらのことから、発生初期のマウスの卵子と胚は、常にprogesterone、 17α-hydroxyprogesterone. 17α,20β-hydroxyprogesterone、 20α-hydroxyprogesteroneおよび20β-hydroxyprogesteroneの合成を行っており、これらプロジェスタジェンの合成能は、胚の初期発生に伴って変化しないことが考えられた。
書誌情報 新潟大学農学部研究報告
en : 新潟大学農学部研究報告

巻 55, 号 2, p. 91-97, 発行日 2003-03
出版者
出版者 新潟大学農学部
ISSN
収録物識別子タイプ ISSN
収録物識別子 03858634
書誌レコードID
収録物識別子タイプ NCID
収録物識別子 AN00183393
著者版フラグ
値 publisher
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