{"_buckets": {"deposit": "04970a15-fd50-4157-addc-890561b29690"}, "_deposit": {"id": "28498", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "28498"}, "status": "published"}, "_oai": {"id": "oai:niigata-u.repo.nii.ac.jp:00028498", "sets": ["456", "1611"]}, "item_7_alternative_title_1": {"attribute_name": "その他のタイトル", "attribute_value_mlt": [{"subitem_alternative_title": "発生初期のマウスの卵子と初期胚におけるプロジェスタジェン合成に関する組織化学的研究"}]}, "item_7_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2003-03", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "2", "bibliographicPageEnd": "97", "bibliographicPageStart": "91", "bibliographicVolumeNumber": "55", "bibliographic_titles": [{"bibliographic_title": "新潟大学農学部研究報告"}, {"bibliographic_title": "新潟大学農学部研究報告", "bibliographic_titleLang": "en"}]}]}, "item_7_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "The activities of △^5-3β-hydroxysteroid dehydrogenase (△^5-3β-HSD) (pregnenolone and 17α-hydroxypregnenolone as the substrates), 20α-HSD (20α-hydroxyprogesterone as the substrate) and 20β-HSD (17α-hydroxyprogesterone and 20β-hydroxyprogesterone as the substrates) were histochemically demonstrated in mouse eggs and embryos, and the progestagen production in those during the early development was examined. In unfertilized mouse eggs, the activities of such HSDs were observed in 88 to 98% of those. The percentages of embryos showing the activities of such HSDs did not change during the early development. It was also histochemically confirmed that the activity of △^5-3β HSD with DHA as the substrate, which is involved in steroid biosynthesis, was observed in 86 to 100% of the eggs and embryos from the un fertilized to blastocyst stages. From the present findings, it was suggested that the progestagens such as progesterone, 17α-hydroxyprogesterone, 17α,20β-dihydroxyprogesterone, 20α-hydroxyprogesterone and 20β-hydroxyprogesterone are constantly synthesized in mouse eggs and embryos during the early development.", "subitem_description_type": "Abstract"}, {"subitem_description": "マウスの未受精卵子および前核期から胚盤抱期までの初期胚について、△^5-3β-HSD(基質としてpregnenoloneおよび17α-hydroxypregnenoloneを使用)、 20α-HSD (20α-hydroxyprogesterone)および20β-HSD (17α-hydroxyprogesterone、 20β-hydroxyprogesterone)の活性を組織化学的に検出し、プロジェスタジェン合成能を検肘した。未受精期の卵子において、これらHSDの活性は88ないし98%のものに認められた。また、前核期から胚盤抱期までの初期胚においても、 83%以上でこれらのHSD活性が検出された。一方、ステロイド生合成の指標となる△^5-3β-HSD (DHA)の活性は、 86%以上の卵子と初期胚に常に観察された。これらのことから、発生初期のマウスの卵子と胚は、常にprogesterone、 17α-hydroxyprogesterone. 17α,20β-hydroxyprogesterone、 20α-hydroxyprogesteroneおよび20β-hydroxyprogesteroneの合成を行っており、これらプロジェスタジェンの合成能は、胚の初期発生に伴って変化しないことが考えられた。", "subitem_description_type": "Abstract"}]}, "item_7_full_name_3": {"attribute_name": "著者別名", "attribute_value_mlt": [{"nameIdentifiers": [{"nameIdentifier": "163935", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "新村, 末雄"}]}, {"nameIdentifiers": [{"nameIdentifier": "163936", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "川上, 心也"}]}]}, "item_7_publisher_7": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "新潟大学農学部"}]}, "item_7_select_19": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_7_source_id_11": {"attribute_name": "書誌レコードID", "attribute_value_mlt": [{"subitem_source_identifier": "AN00183393", "subitem_source_identifier_type": "NCID"}]}, "item_7_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "03858634", "subitem_source_identifier_type": "ISSN"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Niimura, Sueo"}], "nameIdentifiers": [{"nameIdentifier": "163933", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kawakami, Shin-ya"}], "nameIdentifiers": [{"nameIdentifier": "163934", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-08-20"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "55_2_91-97.pdf", "filesize": [{"value": "490.1 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 490100.0, "url": {"label": "55_2_91-97.pdf", "url": "https://niigata-u.repo.nii.ac.jp/record/28498/files/55_2_91-97.pdf"}, "version_id": "4a2f487a-bd47-4f50-b10b-896debd98468"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "mouse embryo", "subitem_subject_scheme": "Other"}, {"subitem_subject": "early development", "subitem_subject_scheme": "Other"}, {"subitem_subject": "hydroxysteroid dehydrogenase", "subitem_subject_scheme": "Other"}, {"subitem_subject": "progestagen production", "subitem_subject_scheme": "Other"}, {"subitem_subject": "histochemistry", "subitem_subject_scheme": "Other"}, {"subitem_subject": "マウス胚", "subitem_subject_scheme": "Other"}, {"subitem_subject": "初期発生", "subitem_subject_scheme": "Other"}, {"subitem_subject": "ヒドロキシステロイド脱水素酵素", "subitem_subject_scheme": "Other"}, {"subitem_subject": "プロジェスタジェン合成", "subitem_subject_scheme": "Other"}, {"subitem_subject": "組織化学", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Histochemical Studies on progestagen Production in Mouse Eggs and Embryos during the Early Development", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Histochemical Studies on progestagen Production in Mouse Eggs and Embryos during the Early Development"}, {"subitem_title": "Histochemical Studies on progestagen Production in Mouse Eggs and Embryos during the Early Development", "subitem_title_language": "en"}]}, "item_type_id": "7", "owner": "1", "path": ["456", "1611"], "permalink_uri": "http://hdl.handle.net/10191/25112", "pubdate": {"attribute_name": "公開日", "attribute_value": "2014-02-12"}, "publish_date": "2014-02-12", "publish_status": "0", "recid": "28498", "relation": {}, "relation_version_is_last": true, "title": ["Histochemical Studies on progestagen Production in Mouse Eggs and Embryos during the Early Development"], "weko_shared_id": null}