{"_buckets": {"deposit": "33f8c9d6-297b-469b-a551-735eab3b9f6c"}, "_deposit": {"id": "28495", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "28495"}, "status": "published"}, "_oai": {"id": "oai:niigata-u.repo.nii.ac.jp:00028495", "sets": ["456", "1610"]}, "item_7_alternative_title_1": {"attribute_name": "その他のタイトル", "attribute_value_mlt": [{"subitem_alternative_title": "Nuclear Maturation and 20α-Hydroxysteroid Dehydrogenase Activity in Mouse Oocytes Treated with 3-Isobutyl-1-Methylxanthine"}]}, "item_7_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2003-08", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "1", "bibliographicPageEnd": "24", "bibliographicPageStart": "17", "bibliographicVolumeNumber": "56", "bibliographic_titles": [{"bibliographic_title": "新潟大学農学部研究報告"}, {"bibliographic_title": "新潟大学農学部研究報告", "bibliographic_titleLang": "en"}]}]}, "item_7_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "cAMPの分解酵素であるホスホジエステラーゼの活性を阻害する作用を有する3-イソブチル-1-メチルキサンチン(IBMX)で処置したマウス卵母細胞について, 核の成熟状態を観察するとともに, 20α-ヒドロキシステロイド脱水素酵素(20α-HSD)の活性を調べ, 卵母細胞の核の成熟と細胞質での20α-ヒドロキシプロゲステロン代謝との関係の有無を検討した。成熟分裂を再開した卵母細胞の割合は, 50μMの濃度のIBMXで処置したものでは3.0%であり, IBMX処置していない対照の卵母細胞の100%(こ比べ, 有意に低かった。一方, 20α-HSD活性は, IBMX処置した卵母細胞と対照の無処置卵母細胞のすべてに認められ, 両者の間で活性に相違はみられなかった。以上の結果から, マウス卵母細胞の核の成熟と細胞質での20α-ヒドロキシプロゲステロン代謝との間には関係のないことが確認された。", "subitem_description_type": "Abstract"}, {"subitem_description": "The state of nuclear maturation and the activity of 20α-hydroxysteroid dehydrogenase (20α-HSD) were observed in mouse oocytes treated with 3-isobutyl-1-methylxanthine (IBMX), an inhibitor of 3\u0027, 5\u0027-cyclic nucleotide phosphodiesterase, and the relationship between nuclear maturation and 20α-hydroxyprogesterone metabolism in the cytoplasm was examined. Nuclei of the oocytes cultured with 50μM IBMX for 8 hrs were almost in the germinal vesicle stage (97%), while those of control oocytes cultured without IBMX were in the metaphase I to telophase I stages, mostly metaphase I stage (68%). The percentage of oocytes in the germinal vesicle stage was significantly higher in the IBMX-treated group than in control group. On the other hand, the activity of 20α-HSDwas demonstrated in all oocytes of both IBMX-treated and non-treated groups. From the present findings, it was confirmed in mouse oocytes that the 20α-hydroxyprogesterone metabolism in the cytoplasm is not related to nuclear maturation.", "subitem_description_type": "Abstract"}]}, "item_7_full_name_3": {"attribute_name": "著者別名", "attribute_value_mlt": [{"nameIdentifiers": [{"nameIdentifier": "163909", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "Niimura, Sueo"}]}, {"nameIdentifiers": [{"nameIdentifier": "163910", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "Saito, Chisato"}]}]}, "item_7_publisher_7": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "新潟大学農学部"}]}, "item_7_select_19": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_7_source_id_11": {"attribute_name": "書誌レコードID", "attribute_value_mlt": [{"subitem_source_identifier": "AN00183393", "subitem_source_identifier_type": "NCID"}]}, "item_7_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "03858634", "subitem_source_identifier_type": "ISSN"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "新村, 末雄"}], "nameIdentifiers": [{"nameIdentifier": "163907", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "斎藤, 千智"}], "nameIdentifiers": [{"nameIdentifier": "163908", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-08-20"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "56_1_17-24.pdf", "filesize": [{"value": "663.5 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 663500.0, "url": {"label": "56_1_17-24.pdf", "url": "https://niigata-u.repo.nii.ac.jp/record/28495/files/56_1_17-24.pdf"}, "version_id": "b44e4a6e-d553-4af7-8afa-565483cacd71"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "マウス卵母細胞", "subitem_subject_scheme": "Other"}, {"subitem_subject": "成熟分裂", "subitem_subject_scheme": "Other"}, {"subitem_subject": "20α-ヒドロキシステロイド代謝", "subitem_subject_scheme": "Other"}, {"subitem_subject": "3-イソブチル- 1 -メチルキサンチン", "subitem_subject_scheme": "Other"}, {"subitem_subject": "組織化学", "subitem_subject_scheme": "Other"}, {"subitem_subject": "mouse oocyte", "subitem_subject_scheme": "Other"}, {"subitem_subject": "meiotic maturation", "subitem_subject_scheme": "Other"}, {"subitem_subject": "20α-hydroxyprogesterone metabolism", "subitem_subject_scheme": "Other"}, {"subitem_subject": "3-isobutyl-1-methylxanthine", "subitem_subject_scheme": "Other"}, {"subitem_subject": "histochemistry", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "jpn"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "3-イソブチル-1-メチルキサンチン処置したマウス卵母細胞の成熟と20α-ヒドロキシステロイド脱水素酵素活性", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "3-イソブチル-1-メチルキサンチン処置したマウス卵母細胞の成熟と20α-ヒドロキシステロイド脱水素酵素活性"}, {"subitem_title": "3-イソブチル-1-メチルキサンチン処置したマウス卵母細胞の成熟と20α-ヒドロキシステロイド脱水素酵素活性", "subitem_title_language": "en"}]}, "item_type_id": "7", "owner": "1", "path": ["456", "1610"], "permalink_uri": "http://hdl.handle.net/10191/25126", "pubdate": {"attribute_name": "公開日", "attribute_value": "2014-02-12"}, "publish_date": "2014-02-12", "publish_status": "0", "recid": "28495", "relation": {}, "relation_version_is_last": true, "title": ["3-イソブチル-1-メチルキサンチン処置したマウス卵母細胞の成熟と20α-ヒドロキシステロイド脱水素酵素活性"], "weko_shared_id": null}