{"_buckets": {"deposit": "33f8c9d6-297b-469b-a551-735eab3b9f6c"}, "_deposit": {"id": "28495", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "28495"}, "status": "published"}, "_oai": {"id": "oai:niigata-u.repo.nii.ac.jp:00028495"}, "item_7_alternative_title_1": {"attribute_name": "\u305d\u306e\u4ed6\u306e\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_alternative_title": "Nuclear Maturation and 20\u03b1-Hydroxysteroid Dehydrogenase Activity in Mouse Oocytes Treated with 3-Isobutyl-1-Methylxanthine"}]}, "item_7_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2003-08", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "1", "bibliographicPageEnd": "24", "bibliographicPageStart": "17", "bibliographicVolumeNumber": "56", "bibliographic_titles": [{"bibliographic_title": "\u65b0\u6f5f\u5927\u5b66\u8fb2\u5b66\u90e8\u7814\u7a76\u5831\u544a"}, {"bibliographic_title": "\u65b0\u6f5f\u5927\u5b66\u8fb2\u5b66\u90e8\u7814\u7a76\u5831\u544a", "bibliographic_titleLang": "en"}]}]}, "item_7_description_4": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "cAMP\u306e\u5206\u89e3\u9175\u7d20\u3067\u3042\u308b\u30db\u30b9\u30db\u30b8\u30a8\u30b9\u30c6\u30e9\u30fc\u30bc\u306e\u6d3b\u6027\u3092\u963b\u5bb3\u3059\u308b\u4f5c\u7528\u3092\u6709\u3059\u308b3-\u30a4\u30bd\u30d6\u30c1\u30eb-1-\u30e1\u30c1\u30eb\u30ad\u30b5\u30f3\u30c1\u30f3(IBMX)\u3067\u51e6\u7f6e\u3057\u305f\u30de\u30a6\u30b9\u5375\u6bcd\u7d30\u80de\u306b\u3064\u3044\u3066, \u6838\u306e\u6210\u719f\u72b6\u614b\u3092\u89b3\u5bdf\u3059\u308b\u3068\u3068\u3082\u306b, 20\u03b1-\u30d2\u30c9\u30ed\u30ad\u30b7\u30b9\u30c6\u30ed\u30a4\u30c9\u8131\u6c34\u7d20\u9175\u7d20(20\u03b1-HSD)\u306e\u6d3b\u6027\u3092\u8abf\u3079, \u5375\u6bcd\u7d30\u80de\u306e\u6838\u306e\u6210\u719f\u3068\u7d30\u80de\u8cea\u3067\u306e20\u03b1-\u30d2\u30c9\u30ed\u30ad\u30b7\u30d7\u30ed\u30b2\u30b9\u30c6\u30ed\u30f3\u4ee3\u8b1d\u3068\u306e\u95a2\u4fc2\u306e\u6709\u7121\u3092\u691c\u8a0e\u3057\u305f\u3002\u6210\u719f\u5206\u88c2\u3092\u518d\u958b\u3057\u305f\u5375\u6bcd\u7d30\u80de\u306e\u5272\u5408\u306f, 50\u03bcM\u306e\u6fc3\u5ea6\u306eIBMX\u3067\u51e6\u7f6e\u3057\u305f\u3082\u306e\u3067\u306f3.0%\u3067\u3042\u308a, IBMX\u51e6\u7f6e\u3057\u3066\u3044\u306a\u3044\u5bfe\u7167\u306e\u5375\u6bcd\u7d30\u80de\u306e100%(\u3053\u6bd4\u3079, \u6709\u610f\u306b\u4f4e\u304b\u3063\u305f\u3002\u4e00\u65b9, 20\u03b1-HSD\u6d3b\u6027\u306f, IBMX\u51e6\u7f6e\u3057\u305f\u5375\u6bcd\u7d30\u80de\u3068\u5bfe\u7167\u306e\u7121\u51e6\u7f6e\u5375\u6bcd\u7d30\u80de\u306e\u3059\u3079\u3066\u306b\u8a8d\u3081\u3089\u308c, \u4e21\u8005\u306e\u9593\u3067\u6d3b\u6027\u306b\u76f8\u9055\u306f\u307f\u3089\u308c\u306a\u304b\u3063\u305f\u3002\u4ee5\u4e0a\u306e\u7d50\u679c\u304b\u3089, \u30de\u30a6\u30b9\u5375\u6bcd\u7d30\u80de\u306e\u6838\u306e\u6210\u719f\u3068\u7d30\u80de\u8cea\u3067\u306e20\u03b1-\u30d2\u30c9\u30ed\u30ad\u30b7\u30d7\u30ed\u30b2\u30b9\u30c6\u30ed\u30f3\u4ee3\u8b1d\u3068\u306e\u9593\u306b\u306f\u95a2\u4fc2\u306e\u306a\u3044\u3053\u3068\u304c\u78ba\u8a8d\u3055\u308c\u305f\u3002", "subitem_description_type": "Abstract"}, {"subitem_description": "The state of nuclear maturation and the activity of 20\u03b1-hydroxysteroid dehydrogenase (20\u03b1-HSD) were observed in mouse oocytes treated with 3-isobutyl-1-methylxanthine (IBMX), an inhibitor of 3\u0027, 5\u0027-cyclic nucleotide phosphodiesterase, and the relationship between nuclear maturation and 20\u03b1-hydroxyprogesterone metabolism in the cytoplasm was examined. Nuclei of the oocytes cultured with 50\u03bcM IBMX for 8 hrs were almost in the germinal vesicle stage (97%), while those of control oocytes cultured without IBMX were in the metaphase I to telophase I stages, mostly metaphase I stage (68%). The percentage of oocytes in the germinal vesicle stage was significantly higher in the IBMX-treated group than in control group. On the other hand, the activity of 20\u03b1-HSDwas demonstrated in all oocytes of both IBMX-treated and non-treated groups. From the present findings, it was confirmed in mouse oocytes that the 20\u03b1-hydroxyprogesterone metabolism in the cytoplasm is not related to nuclear maturation.", "subitem_description_type": "Abstract"}]}, "item_7_full_name_3": {"attribute_name": "\u8457\u8005\u5225\u540d", "attribute_value_mlt": [{"nameIdentifiers": [{"nameIdentifier": "163909", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "Niimura, Sueo"}]}, {"nameIdentifiers": [{"nameIdentifier": "163910", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "Saito, Chisato"}]}]}, "item_7_publisher_7": {"attribute_name": "\u51fa\u7248\u8005", "attribute_value_mlt": [{"subitem_publisher": "\u65b0\u6f5f\u5927\u5b66\u8fb2\u5b66\u90e8"}]}, "item_7_select_19": {"attribute_name": "\u8457\u8005\u7248\u30d5\u30e9\u30b0", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_7_source_id_11": {"attribute_name": "\u66f8\u8a8c\u30ec\u30b3\u30fc\u30c9ID", "attribute_value_mlt": [{"subitem_source_identifier": 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