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  1. 0 資料タイプ別
  2. 03 紀要論文
  1. 070 農学部
  2. 20 紀要
  3. 01 新潟大学農学部研究報告
  4. 第56巻第1号

3-イソブチル-1-メチルキサンチン処置したマウス卵母細胞の成熟と20α-ヒドロキシステロイド脱水素酵素活性

http://hdl.handle.net/10191/25126
9a5909ec-c8d6-4825-b62f-b6b8fab8408e
名前 / ファイル ライセンス アクション
56_1_17-24.pdf 56_1_17-24.pdf (663.5 kB)
Item type 紀要論文 / Departmental Bulletin Paper(1)
公開日 2014-02-12
タイトル
タイトル 3-イソブチル-1-メチルキサンチン処置したマウス卵母細胞の成熟と20α-ヒドロキシステロイド脱水素酵素活性
タイトル
言語 en
タイトル 3-イソブチル-1-メチルキサンチン処置したマウス卵母細胞の成熟と20α-ヒドロキシステロイド脱水素酵素活性
言語
言語 jpn
キーワード
主題Scheme Other
主題 マウス卵母細胞
キーワード
主題Scheme Other
主題 成熟分裂
キーワード
主題Scheme Other
主題 20α-ヒドロキシステロイド代謝
キーワード
主題Scheme Other
主題 3-イソブチル- 1 -メチルキサンチン
キーワード
主題Scheme Other
主題 組織化学
キーワード
主題Scheme Other
主題 mouse oocyte
キーワード
主題Scheme Other
主題 meiotic maturation
キーワード
主題Scheme Other
主題 20α-hydroxyprogesterone metabolism
キーワード
主題Scheme Other
主題 3-isobutyl-1-methylxanthine
キーワード
主題Scheme Other
主題 histochemistry
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ departmental bulletin paper
その他のタイトル
その他のタイトル Nuclear Maturation and 20α-Hydroxysteroid Dehydrogenase Activity in Mouse Oocytes Treated with 3-Isobutyl-1-Methylxanthine
著者 新村, 末雄

× 新村, 末雄

WEKO 163907

新村, 末雄

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斎藤, 千智

× 斎藤, 千智

WEKO 163908

斎藤, 千智

Search repository
著者別名
識別子
識別子 163909
識別子Scheme WEKO
姓名
姓名 Niimura, Sueo
著者別名
識別子
識別子 163910
識別子Scheme WEKO
姓名
姓名 Saito, Chisato
抄録
内容記述タイプ Abstract
内容記述 cAMPの分解酵素であるホスホジエステラーゼの活性を阻害する作用を有する3-イソブチル-1-メチルキサンチン(IBMX)で処置したマウス卵母細胞について, 核の成熟状態を観察するとともに, 20α-ヒドロキシステロイド脱水素酵素(20α-HSD)の活性を調べ, 卵母細胞の核の成熟と細胞質での20α-ヒドロキシプロゲステロン代謝との関係の有無を検討した。成熟分裂を再開した卵母細胞の割合は, 50μMの濃度のIBMXで処置したものでは3.0%であり, IBMX処置していない対照の卵母細胞の100%(こ比べ, 有意に低かった。一方, 20α-HSD活性は, IBMX処置した卵母細胞と対照の無処置卵母細胞のすべてに認められ, 両者の間で活性に相違はみられなかった。以上の結果から, マウス卵母細胞の核の成熟と細胞質での20α-ヒドロキシプロゲステロン代謝との間には関係のないことが確認された。
抄録
内容記述タイプ Abstract
内容記述 The state of nuclear maturation and the activity of 20α-hydroxysteroid dehydrogenase (20α-HSD) were observed in mouse oocytes treated with 3-isobutyl-1-methylxanthine (IBMX), an inhibitor of 3', 5'-cyclic nucleotide phosphodiesterase, and the relationship between nuclear maturation and 20α-hydroxyprogesterone metabolism in the cytoplasm was examined. Nuclei of the oocytes cultured with 50μM IBMX for 8 hrs were almost in the germinal vesicle stage (97%), while those of control oocytes cultured without IBMX were in the metaphase I to telophase I stages, mostly metaphase I stage (68%). The percentage of oocytes in the germinal vesicle stage was significantly higher in the IBMX-treated group than in control group. On the other hand, the activity of 20α-HSDwas demonstrated in all oocytes of both IBMX-treated and non-treated groups. From the present findings, it was confirmed in mouse oocytes that the 20α-hydroxyprogesterone metabolism in the cytoplasm is not related to nuclear maturation.
書誌情報 新潟大学農学部研究報告
en : 新潟大学農学部研究報告

巻 56, 号 1, p. 17-24, 発行日 2003-08
出版者
出版者 新潟大学農学部
ISSN
収録物識別子タイプ ISSN
収録物識別子 03858634
書誌レコードID
収録物識別子タイプ NCID
収録物識別子 AN00183393
著者版フラグ
値 publisher
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