{"_buckets": {"deposit": "82afb24f-9580-4678-88a6-6acc65bf5098"}, "_deposit": {"id": "28474", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "28474"}, "status": "published"}, "_oai": {"id": "oai:niigata-u.repo.nii.ac.jp:00028474"}, "item_7_alternative_title_1": {"attribute_name": "\u305d\u306e\u4ed6\u306e\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_alternative_title": "\u30b7\u30e7\u7cd6\u5bc6\u5ea6\u52fe\u914d\u9060\u5fc3\u6cd5\u3092\u7528\u3044\u305f\u5b89\u5b9a\u30ea\u30dd\u30bd\u30fc\u30e0\u306e\u4f5c\u88fd\u3068Cry1A\u6bd2\u7d20\u3068\u306e\u53cd\u5fdc\u6027\u306e\u8a55\u4fa1"}]}, "item_7_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2005-03", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "2", "bibliographicPageEnd": "120", "bibliographicPageStart": "115", "bibliographicVolumeNumber": "57", "bibliographic_titles": [{"bibliographic_title": "\u65b0\u6f5f\u5927\u5b66\u8fb2\u5b66\u90e8\u7814\u7a76\u5831\u544a"}, {"bibliographic_title": "\u65b0\u6f5f\u5927\u5b66\u8fb2\u5b66\u90e8\u7814\u7a76\u5831\u544a", "bibliographic_titleLang": "en"}]}]}, "item_7_description_4": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "Liposomes were constructed with phosphatidylcholine (PC) and fluorescence indicator, calcein, and purified using 9-40% discontinuous sucrose density gradients centrifugation (SDGC) or gel filtration column chromatography (GFCC). Stability and sensitivity of those liposomes to insecticidal Cry1A toxins of Bacillus thuringiensis were compared each other and one purified by GFCC was shown to leak calcein by 40% of total fluorescence contents during 96 hr storage. On the other hand, liposome purified by SDGC was more stable and almost no leakage observed during the same period. Cry1Ab of B. thuringiensis weakly reacted with the liposome purified by GFCC and 2.5% of total encapsulated fluorescence was released, but Cry1Aa and Cry1Ac did not. Contrarily, liposome purified by SDGC was highly reactive with Cry1Ab and 40% of the calcein was released and 15%-release by Cry1Aa and Cry1Ac were also observed. These data clearly indicated that liposomes purified by SDGC were more stable and highly reactive to Cry toxins compared to that purified by GFCC. These liposomes are useful tool to analyze the interaction between Cry1A toxins and lipid membranes.\u30db\u30b9\u30d5\u30a1\u30c1\u30b8\u30eb\u30b3\u30ea\u30f3(PC)\u3092\u7528\u3044\u3066\u30ea\u30dd\u30bd\u30fc\u30e0\u3092\u69cb\u7bc9\u3057\u3001\u5185\u90e8\u7a7a\u9593\u306b\u86cd\u5149\u8272\u7d20\u3001\u30ab\u30eb\u30bb\u30a4\u30f3\u3092\u5c01\u5165\u3057\u305f\u3002PC\u30ea\u30dd\u30bd\u30fc\u30e0\u3092\u30b7\u30e7\u7cd6\u5bc6\u5ea6\u52fe\u914d\u9060\u5fc3\u6cd5(SDGC)\u3042\u308b\u3044\u306f\u30b2\u30eb\u308d\u904e\u30ab\u30e9\u30e0\u30af\u30ed\u30de\u30c8\u30e9\u30b0\u30e9\u30d5\u30a3\u30fc(GFCC)\u3067\u7cbe\u88fd\u3057\u3001\u5404\u3005\u306e\u7cbe\u88fdPC\u30ea\u30dd\u30bd\u30fc\u30e0\u306b\u3064\u3044\u3066\u3001\u6eb6\u6db2\u4e2d\u3067\u306e\u5b89\u5b9a\u6027\u3068Bacillus thuringiensis\u306eCry1A\u6bba\u866b\u6bd2\u7d20\u3068\u306e\u53cd\u5fdc\u6027\u3092\u6bd4\u8f03\u89e3\u6790\u3057\u305f\u3002GFCC\u3067\u7cbe\u88fd\u3057\u305fPC\u30ea\u30dd\u30bd\u30fc\u30e0\u306f\u4e0d\u5b89\u5b9a\u3067\u300196\u6642\u9593\u4fdd\u5b58\u3059\u308b\u3068\u5185\u5305\u30ab\u30eb\u30bb\u30a4\u30f3\u306e40%\u304c\u30ea\u30dd\u30bd\u30fc\u30e0\u306e\u5916\u306b\u6f0f\u51fa\u3057\u305f\u3002\u4e00\u65b9\u3001SDGC\u7cbe\u88fdPC\u30ea\u30dd\u30bd\u30fc\u30e0\u306f\u5b89\u5b9a\u306796\u6642\u9593\u4fdd\u5b58\u5f8c\u3082\u307b\u3068\u3093\u3069\u30ab\u30eb\u30bb\u30a4\u30f3\u306e\u6f0f\u51fa\u3092\u793a\u3055\u306a\u304b\u3063\u305f\u3002\u6bba\u866b\u6bd2\u7d20Cry1Ab\u306fGFCC\u7cbe\u88fdPC\u30ea\u30dd\u30bd\u30fc\u30e0\u819c\u306b\u5c0f\u5b54\u5f62\u6210\u3092\u793a\u3057\u305f\u304c\u3001\u30ab\u30eb\u30bb\u30a4\u30f3\u306e\u6f0f\u51fa\u306f2.5%\u306b\u3068\u3069\u307e\u308a\u3001\u5c0f\u5b54\u5f62\u6210\u7387\u306f\u975e\u5e38\u306b\u4f4e\u3044\u3068\u601d\u308f\u308c\u305f\u3002\u307e\u305fCry1Aa\u3068Cry1Ac\u306f\u5171\u306b\u5c0f\u5b54\u3092\u5f62\u6210\u3057\u306a\u304b\u3063\u305f\u3002\u4e00\u65b9\u3001SDGC\u7cbe\u88fdPC\u30ea\u30dd\u30bd\u30fc\u30e0\u306fCry1Ab\u3068\u53cd\u5fdc\u3057\u300140%\u306e\u30ab\u30eb\u30bb\u30a4\u30f3\u6f0f\u51fa\u304c\u691c\u51fa\u3055\u308c\u305f\u3002Cry1Aa\u3068Cry1Ac\u3068\u306e\u53cd\u5fdc\u306b\u969b\u3057\u3066\u308215%\u306e\u30ab\u30eb\u30bb\u30a4\u30f3\u6f0f\u51fa\u304c\u691c\u51fa\u3055\u308c\u305f\u3002\u3053\u308c\u3089\u306e\u5b9f\u9a13\u7d50\u679c\u306f\u3001SDGC\u7cbe\u88fdPC\u30ea\u30dd\u30bd\u30fc\u30e0\u306e\u65b9\u304cGFCC\u7cbe\u88fdPC\u30ea\u30dd\u30bd\u30fc\u30e0\u3088\u308a\u3082\u5b89\u5b9a\u3067\u3001Cry1A\u6bd2\u7d20\u3068\u306e\u53cd\u5fdc\u6027\u3082\u9ad8\u3044\u3053\u3068\u3092\u793a\u3057\u3001\u6bd4\u91cd\u306b\u57fa\u3065\u304d\u30ea\u30dd\u30bd\u30fc\u30e0\u3092\u7cbe\u88fd\u3059\u308b\u3053\u3068\u304c\u5747\u4e00\u306a\u5b89\u5b9a\u30ea\u30dd\u30bd\u30fc\u30e0\u3092\u5f97\u308b\u3046\u3048\u3067\u6709\u52b9\u3067\u3042\u308b\u3053\u3068\u3092\u793a\u3057\u305f\u3002SDGC\u7cbe\u88fdPC\u30ea\u30dd\u30bd\u30fc\u30e0\u306fCry1A\u6bd2\u7d20\u3068\u8102\u8cea\u819c\u3068\u306e\u76f8\u4e92\u4f5c\u7528\u3092\u89e3\u6790\u3059\u308b\u4e0a\u3067\u6709\u52b9\u3067\u3042\u308b\u3002", 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[{"creatorNames": [{"creatorName": "Haginoya, Kohsuke"}], "nameIdentifiers": [{"nameIdentifier": "163783", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kato, Taisuke"}], "nameIdentifiers": [{"nameIdentifier": "163784", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Higuchi, Masahiro"}], "nameIdentifiers": [{"nameIdentifier": "163785", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Shitomi, Yasuyuki"}], "nameIdentifiers": [{"nameIdentifier": "163786", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Asakura, Tsuyoshi"}], "nameIdentifiers": [{"nameIdentifier": "163787", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Hayakawa, Tohru"}], "nameIdentifiers": [{"nameIdentifier": "163788", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Mitsui, Toshiaki"}], "nameIdentifiers": [{"nameIdentifier": "163789", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Hori, Hidetaka"}], "nameIdentifiers": [{"nameIdentifier": "163790", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-08-20"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "17_0017.pdf", "filesize": [{"value": "364.8 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 364800.0, "url": {"label": "17_0017.pdf", "url": "https://niigata-u.repo.nii.ac.jp/record/28474/files/17_0017.pdf"}, "version_id": "290bee1a-3099-4392-8557-becb2285b9c7"}]}, "item_keyword": {"attribute_name": "\u30ad\u30fc\u30ef\u30fc\u30c9", "attribute_value_mlt": [{"subitem_subject": "Phosphatidylcholine (PC)", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Liposome", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Insecticidal Cry1A toxins", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Bacillus thuringiensis", "subitem_subject_scheme": "Other"}, {"subitem_subject": "calcein", "subitem_subject_scheme": "Other"}, {"subitem_subject": "interaction between liposome and Cry1Ab", "subitem_subject_scheme": "Other"}, {"subitem_subject": "\u30db\u30b9\u30d5\u30a1\u30c1\u30b8\u30eb\u30b3\u30ea\u30f3(PC)", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "\u30ea\u30dd\u30bd\u30fc\u30e0", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Cry1A\u6bba\u866b\u30c8\u30ad\u30b7\u30f3", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Bacillus thuringiensis", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "\u86cd\u5149\u8272\u7d20\u30ab\u30eb\u30bb\u30a4\u30f3", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "\u30ea\u30dd\u30bd\u30fc\u30e0\u3068Cry1Ab\u30c8\u30ad\u30b7\u30f3\u3068\u306e\u76f8\u4e92\u4f5c\u7528", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Preparation of Stable Liposomes Using Sucrose Density Gradient Centrifugation and Their Interaction with Insecticidal Cry1A Toxins of Bacillus thuringiensis", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "Preparation of Stable Liposomes Using Sucrose Density Gradient Centrifugation and Their Interaction with Insecticidal Cry1A Toxins of Bacillus thuringiensis"}, {"subitem_title": "Preparation of Stable Liposomes Using Sucrose Density Gradient Centrifugation and Their Interaction with Insecticidal Cry1A Toxins of Bacillus thuringiensis", "subitem_title_language": "en"}]}, "item_type_id": "7", "owner": "1", "path": ["453/456", "485/872/1583/1607"], "permalink_uri": "http://hdl.handle.net/10191/685", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_value": "2007-04-18"}, "publish_date": "2007-04-18", "publish_status": "0", "recid": "28474", "relation": {}, "relation_version_is_last": true, "title": ["Preparation of Stable Liposomes Using Sucrose Density Gradient Centrifugation and Their Interaction with Insecticidal Cry1A Toxins of Bacillus thuringiensis"], "weko_shared_id": null}