ログイン
言語:

WEKO3

  • トップ
  • ランキング
To
lat lon distance
To

Field does not validate



インデックスリンク

インデックスツリー

メールアドレスを入力してください。

WEKO

One fine body…

WEKO

One fine body…

アイテム

{"_buckets": {"deposit": "82afb24f-9580-4678-88a6-6acc65bf5098"}, "_deposit": {"id": "28474", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "28474"}, "status": "published"}, "_oai": {"id": "oai:niigata-u.repo.nii.ac.jp:00028474", "sets": ["456", "1607"]}, "item_7_alternative_title_1": {"attribute_name": "その他のタイトル", "attribute_value_mlt": [{"subitem_alternative_title": "ショ糖密度勾配遠心法を用いた安定リポソームの作製とCry1A毒素との反応性の評価"}]}, "item_7_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2005-03", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "2", "bibliographicPageEnd": "120", "bibliographicPageStart": "115", "bibliographicVolumeNumber": "57", "bibliographic_titles": [{"bibliographic_title": "新潟大学農学部研究報告"}, {"bibliographic_title": "新潟大学農学部研究報告", "bibliographic_titleLang": "en"}]}]}, "item_7_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Liposomes were constructed with phosphatidylcholine (PC) and fluorescence indicator, calcein, and purified using 9-40% discontinuous sucrose density gradients centrifugation (SDGC) or gel filtration column chromatography (GFCC). Stability and sensitivity of those liposomes to insecticidal Cry1A toxins of Bacillus thuringiensis were compared each other and one purified by GFCC was shown to leak calcein by 40% of total fluorescence contents during 96 hr storage. On the other hand, liposome purified by SDGC was more stable and almost no leakage observed during the same period. Cry1Ab of B. thuringiensis weakly reacted with the liposome purified by GFCC and 2.5% of total encapsulated fluorescence was released, but Cry1Aa and Cry1Ac did not. Contrarily, liposome purified by SDGC was highly reactive with Cry1Ab and 40% of the calcein was released and 15%-release by Cry1Aa and Cry1Ac were also observed. These data clearly indicated that liposomes purified by SDGC were more stable and highly reactive to Cry toxins compared to that purified by GFCC. These liposomes are useful tool to analyze the interaction between Cry1A toxins and lipid membranes.ホスファチジルコリン(PC)を用いてリポソームを構築し、内部空間に蛍光色素、カルセインを封入した。PCリポソームをショ糖密度勾配遠心法(SDGC)あるいはゲルろ過カラムクロマトラグラフィー(GFCC)で精製し、各々の精製PCリポソームについて、溶液中での安定性とBacillus thuringiensisのCry1A殺虫毒素との反応性を比較解析した。GFCCで精製したPCリポソームは不安定で、96時間保存すると内包カルセインの40%がリポソームの外に漏出した。一方、SDGC精製PCリポソームは安定で96時間保存後もほとんどカルセインの漏出を示さなかった。殺虫毒素Cry1AbはGFCC精製PCリポソーム膜に小孔形成を示したが、カルセインの漏出は2.5%にとどまり、小孔形成率は非常に低いと思われた。またCry1AaとCry1Acは共に小孔を形成しなかった。一方、SDGC精製PCリポソームはCry1Abと反応し、40%のカルセイン漏出が検出された。Cry1AaとCry1Acとの反応に際しても15%のカルセイン漏出が検出された。これらの実験結果は、SDGC精製PCリポソームの方がGFCC精製PCリポソームよりも安定で、Cry1A毒素との反応性も高いことを示し、比重に基づきリポソームを精製することが均一な安定リポソームを得るうえで有効であることを示した。SDGC精製PCリポソームはCry1A毒素と脂質膜との相互作用を解析する上で有効である。", "subitem_description_type": "Abstract"}]}, "item_7_full_name_3": {"attribute_name": "著者別名", "attribute_value_mlt": [{"nameIdentifiers": [{"nameIdentifier": "163791", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "萩野谷, 功輔"}]}, {"nameIdentifiers": [{"nameIdentifier": "163792", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "加藤, 泰介"}]}, {"nameIdentifiers": [{"nameIdentifier": "163793", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "樋口, 昌宏"}]}, {"nameIdentifiers": [{"nameIdentifier": "163794", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "蔀, 泰幸"}]}, {"nameIdentifiers": [{"nameIdentifier": "163795", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "朝倉, 剛"}]}, {"nameIdentifiers": [{"nameIdentifier": "163796", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "早川, 徹"}]}, {"nameIdentifiers": [{"nameIdentifier": "163797", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "三ツ井, 敏明"}]}, {"nameIdentifiers": [{"nameIdentifier": "163798", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "堀, 秀隆"}]}]}, "item_7_publisher_7": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "新潟大学農学部"}]}, "item_7_select_19": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_7_source_id_11": {"attribute_name": "書誌レコードID", "attribute_value_mlt": [{"subitem_source_identifier": "AN00183393", "subitem_source_identifier_type": "NCID"}]}, "item_7_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "03858634", "subitem_source_identifier_type": "ISSN"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Haginoya, Kohsuke"}], "nameIdentifiers": [{"nameIdentifier": "163783", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kato, Taisuke"}], "nameIdentifiers": [{"nameIdentifier": "163784", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Higuchi, Masahiro"}], "nameIdentifiers": [{"nameIdentifier": "163785", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Shitomi, Yasuyuki"}], "nameIdentifiers": [{"nameIdentifier": "163786", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Asakura, Tsuyoshi"}], "nameIdentifiers": [{"nameIdentifier": "163787", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Hayakawa, Tohru"}], "nameIdentifiers": [{"nameIdentifier": "163788", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Mitsui, Toshiaki"}], "nameIdentifiers": [{"nameIdentifier": "163789", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Hori, Hidetaka"}], "nameIdentifiers": [{"nameIdentifier": "163790", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-08-20"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "17_0017.pdf", "filesize": [{"value": "364.8 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 364800.0, "url": {"label": "17_0017.pdf", "url": "https://niigata-u.repo.nii.ac.jp/record/28474/files/17_0017.pdf"}, "version_id": "290bee1a-3099-4392-8557-becb2285b9c7"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "Phosphatidylcholine (PC)", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Liposome", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Insecticidal Cry1A toxins", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Bacillus thuringiensis", "subitem_subject_scheme": "Other"}, {"subitem_subject": "calcein", "subitem_subject_scheme": "Other"}, {"subitem_subject": "interaction between liposome and Cry1Ab", "subitem_subject_scheme": "Other"}, {"subitem_subject": "ホスファチジルコリン(PC)", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "リポソーム", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Cry1A殺虫トキシン", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Bacillus thuringiensis", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "蛍光色素カルセイン", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "リポソームとCry1Abトキシンとの相互作用", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Preparation of Stable Liposomes Using Sucrose Density Gradient Centrifugation and Their Interaction with Insecticidal Cry1A Toxins of Bacillus thuringiensis", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Preparation of Stable Liposomes Using Sucrose Density Gradient Centrifugation and Their Interaction with Insecticidal Cry1A Toxins of Bacillus thuringiensis"}, {"subitem_title": "Preparation of Stable Liposomes Using Sucrose Density Gradient Centrifugation and Their Interaction with Insecticidal Cry1A Toxins of Bacillus thuringiensis", "subitem_title_language": "en"}]}, "item_type_id": "7", "owner": "1", "path": ["456", "1607"], "permalink_uri": "http://hdl.handle.net/10191/685", "pubdate": {"attribute_name": "公開日", "attribute_value": "2007-04-18"}, "publish_date": "2007-04-18", "publish_status": "0", "recid": "28474", "relation": {}, "relation_version_is_last": true, "title": ["Preparation of Stable Liposomes Using Sucrose Density Gradient Centrifugation and Their Interaction with Insecticidal Cry1A Toxins of Bacillus thuringiensis"], "weko_shared_id": null}
  1. 0 資料タイプ別
  2. 03 紀要論文
  1. 070 農学部
  2. 20 紀要
  3. 01 新潟大学農学部研究報告
  4. 第57巻第2号

Preparation of Stable Liposomes Using Sucrose Density Gradient Centrifugation and Their Interaction with Insecticidal Cry1A Toxins of Bacillus thuringiensis

http://hdl.handle.net/10191/685
http://hdl.handle.net/10191/685
5a59bc93-b2bf-4463-a0e8-a6c01ab3d8a2
名前 / ファイル ライセンス アクション
17_0017.pdf 17_0017.pdf (364.8 kB)
Item type 紀要論文 / Departmental Bulletin Paper(1)
公開日 2007-04-18
タイトル
タイトル Preparation of Stable Liposomes Using Sucrose Density Gradient Centrifugation and Their Interaction with Insecticidal Cry1A Toxins of Bacillus thuringiensis
タイトル
言語 en
タイトル Preparation of Stable Liposomes Using Sucrose Density Gradient Centrifugation and Their Interaction with Insecticidal Cry1A Toxins of Bacillus thuringiensis
言語
言語 eng
キーワード
主題Scheme Other
主題 Phosphatidylcholine (PC)
キーワード
主題Scheme Other
主題 Liposome
キーワード
主題Scheme Other
主題 Insecticidal Cry1A toxins
キーワード
主題Scheme Other
主題 Bacillus thuringiensis
キーワード
主題Scheme Other
主題 calcein
キーワード
主題Scheme Other
主題 interaction between liposome and Cry1Ab
キーワード
言語 en
主題Scheme Other
主題 ホスファチジルコリン(PC)
キーワード
言語 en
主題Scheme Other
主題 リポソーム
キーワード
言語 en
主題Scheme Other
主題 Cry1A殺虫トキシン
キーワード
言語 en
主題Scheme Other
主題 Bacillus thuringiensis
キーワード
言語 en
主題Scheme Other
主題 蛍光色素カルセイン
キーワード
言語 en
主題Scheme Other
主題 リポソームとCry1Abトキシンとの相互作用
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ departmental bulletin paper
その他のタイトル
その他のタイトル ショ糖密度勾配遠心法を用いた安定リポソームの作製とCry1A毒素との反応性の評価
著者 Haginoya, Kohsuke

× Haginoya, Kohsuke

WEKO 163783

Haginoya, Kohsuke

Search repository
Kato, Taisuke

× Kato, Taisuke

WEKO 163784

Kato, Taisuke

Search repository
Higuchi, Masahiro

× Higuchi, Masahiro

WEKO 163785

Higuchi, Masahiro

Search repository
Shitomi, Yasuyuki

× Shitomi, Yasuyuki

WEKO 163786

Shitomi, Yasuyuki

Search repository
Asakura, Tsuyoshi

× Asakura, Tsuyoshi

WEKO 163787

Asakura, Tsuyoshi

Search repository
Hayakawa, Tohru

× Hayakawa, Tohru

WEKO 163788

Hayakawa, Tohru

Search repository
Mitsui, Toshiaki

× Mitsui, Toshiaki

WEKO 163789

Mitsui, Toshiaki

Search repository
Hori, Hidetaka

× Hori, Hidetaka

WEKO 163790

Hori, Hidetaka

Search repository
著者別名
識別子 163791
識別子Scheme WEKO
姓名 萩野谷, 功輔
著者別名
識別子 163792
識別子Scheme WEKO
姓名 加藤, 泰介
著者別名
識別子 163793
識別子Scheme WEKO
姓名 樋口, 昌宏
著者別名
識別子 163794
識別子Scheme WEKO
姓名 蔀, 泰幸
著者別名
識別子 163795
識別子Scheme WEKO
姓名 朝倉, 剛
著者別名
識別子 163796
識別子Scheme WEKO
姓名 早川, 徹
著者別名
識別子 163797
識別子Scheme WEKO
姓名 三ツ井, 敏明
著者別名
識別子 163798
識別子Scheme WEKO
姓名 堀, 秀隆
抄録
内容記述タイプ Abstract
内容記述 Liposomes were constructed with phosphatidylcholine (PC) and fluorescence indicator, calcein, and purified using 9-40% discontinuous sucrose density gradients centrifugation (SDGC) or gel filtration column chromatography (GFCC). Stability and sensitivity of those liposomes to insecticidal Cry1A toxins of Bacillus thuringiensis were compared each other and one purified by GFCC was shown to leak calcein by 40% of total fluorescence contents during 96 hr storage. On the other hand, liposome purified by SDGC was more stable and almost no leakage observed during the same period. Cry1Ab of B. thuringiensis weakly reacted with the liposome purified by GFCC and 2.5% of total encapsulated fluorescence was released, but Cry1Aa and Cry1Ac did not. Contrarily, liposome purified by SDGC was highly reactive with Cry1Ab and 40% of the calcein was released and 15%-release by Cry1Aa and Cry1Ac were also observed. These data clearly indicated that liposomes purified by SDGC were more stable and highly reactive to Cry toxins compared to that purified by GFCC. These liposomes are useful tool to analyze the interaction between Cry1A toxins and lipid membranes.ホスファチジルコリン(PC)を用いてリポソームを構築し、内部空間に蛍光色素、カルセインを封入した。PCリポソームをショ糖密度勾配遠心法(SDGC)あるいはゲルろ過カラムクロマトラグラフィー(GFCC)で精製し、各々の精製PCリポソームについて、溶液中での安定性とBacillus thuringiensisのCry1A殺虫毒素との反応性を比較解析した。GFCCで精製したPCリポソームは不安定で、96時間保存すると内包カルセインの40%がリポソームの外に漏出した。一方、SDGC精製PCリポソームは安定で96時間保存後もほとんどカルセインの漏出を示さなかった。殺虫毒素Cry1AbはGFCC精製PCリポソーム膜に小孔形成を示したが、カルセインの漏出は2.5%にとどまり、小孔形成率は非常に低いと思われた。またCry1AaとCry1Acは共に小孔を形成しなかった。一方、SDGC精製PCリポソームはCry1Abと反応し、40%のカルセイン漏出が検出された。Cry1AaとCry1Acとの反応に際しても15%のカルセイン漏出が検出された。これらの実験結果は、SDGC精製PCリポソームの方がGFCC精製PCリポソームよりも安定で、Cry1A毒素との反応性も高いことを示し、比重に基づきリポソームを精製することが均一な安定リポソームを得るうえで有効であることを示した。SDGC精製PCリポソームはCry1A毒素と脂質膜との相互作用を解析する上で有効である。
書誌情報 新潟大学農学部研究報告
en : 新潟大学農学部研究報告

巻 57, 号 2, p. 115-120, 発行日 2005-03
出版者
出版者 新潟大学農学部
ISSN
収録物識別子タイプ ISSN
収録物識別子 03858634
書誌レコードID
収録物識別子タイプ NCID
収録物識別子 AN00183393
著者版フラグ
値 publisher
戻る
0
views
See details
Views

Versions

Ver.1 2021-03-01 10:59:23.426840
Show All versions

Share

Mendeley Twitter Facebook Print Addthis

Cite as

エクスポート

OAI-PMH
  • OAI-PMH JPCOAR
  • OAI-PMH DublinCore
  • OAI-PMH DDI
Other Formats
  • JSON
  • BIBTEX

Confirm


Powered by WEKO3


Powered by WEKO3