{"_buckets": {"deposit": "aa82293b-c477-4162-9633-18cca6b44938"}, "_deposit": {"id": "28471", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "28471"}, "status": "published"}, "_oai": {"id": "oai:niigata-u.repo.nii.ac.jp:00028471", "sets": ["456", "1607"]}, "item_7_alternative_title_1": {"attribute_name": "その他のタイトル", "attribute_value_mlt": [{"subitem_alternative_title": "ブタ裸化卵母細胞の成熟に及ぼす各種ステロイドの影響"}]}, "item_7_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2005-03", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "2", "bibliographicPageEnd": "102", "bibliographicPageStart": "95", "bibliographicVolumeNumber": "57", "bibliographic_titles": [{"bibliographic_title": "新潟大学農学部研究報告"}, {"bibliographic_title": "新潟大学農学部研究報告", "bibliographic_titleLang": "en"}]}]}, "item_7_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "The state of nuclear maturation and the distribution pattern of cortical granules (CGs) were observed in denuded porcine oocytes cultured with various steroids, in order to identify the effects of steroids on their nuclear and cytoplasmic maturation. At 44 hrs after maturation culture with various steroids, the percentages of oocytes with the metaphase II stage nuclei were significantly higher in those cultured with cholesterol, progesterone or 20 α -hydroxyprogesterone (72.7 to 89.1 %) than in each control oocyte group (49.3 and 58.3 %), whereas the percentages of such oocytes cultured with pregenolone, 17 α -hydroxyprogesterone, 17 α -hydroxypregnenolone and estradiol-17β were similar to those of each control oocyte group. The distribution patterns of CGs in the oocytes cultured with steroids were similar to those in each control oocyte. The developmental rates to 2-cell embryos of inseminated oocytes matured in the medium containing steroids (50.0 to 73.5 %) also did not differ from those of each control oocyte group (60.0 to 69.0 %), even in the use of either steroid examined. From these findings, it was clarified in denuded porcine oocytes that the nuclear maturation to the metaphase II stage is accelerated by the treatment of some progestins and cholesterol, whereas the cytoplasmic maturation and the development after insemination are not affected by the treatment of steroids.卵核胞期のブタ裸化卵母細胞を各種ステロイドを含む培養液で培養し、核の成熟状態と表層粒の分布状態を観察した。各種ステロイドを含む培養液で44時間培養した卵母細胞において、第二成熟分裂中期に達したものの割合は、コレステロール、プロゲステロンあるいは20α-ヒドロキシプロゲステロンと共に培養したものでは72.7ないし89.1%であり、いずれもそれぞれの対照の卵母細胞の割合(49.3および58.3%)に比べて有意に高かったが、プレグネノロン、エストラジオール-17β、17α-ヒドロキシプロゲステロンおよび17α-ヒドロキシプレグネノロンと共に培養したものでは、それぞれの対照の卵母細胞の割合と相違なかった。また、各種ステロイドを含む培養液で22および44時間培養した卵母細胞において、表層粒の分布状態は、対照のステロイド処置していない卵母細胞のものと相違なかった。さらに、各種ステロイドを含む培養液で44時間培養した卵母細胞を媒精して48時間培養したところ、50.0ないし73.5%が2細胞胚に発生したが、2細胞胚への発生率は、いずれもそれぞれの対照の卵母細胞の発生率(60.0ないし69.0%)と相違なかった。以上の結果から、ブタの裸化卵母細胞では、第二成熟分裂中期への核の成熟はプロゲステンのいくつかとコレステロールの処置によって促進されるが、表層粒の分布と媒精後の2細胞胚への発生は各種ステロイドの処置によって影響されないことが明らかにされた。", "subitem_description_type": "Abstract"}]}, "item_7_full_name_3": {"attribute_name": "著者別名", "attribute_value_mlt": [{"nameIdentifiers": [{"nameIdentifier": "163776", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "新村, 末雄"}]}, {"nameIdentifiers": [{"nameIdentifier": "163777", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "吉久, 陽子"}]}, {"nameIdentifiers": [{"nameIdentifier": "163778", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "高野, 裕子"}]}]}, "item_7_publisher_7": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "新潟大学農学部"}]}, "item_7_select_19": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_7_source_id_11": {"attribute_name": "書誌レコードID", "attribute_value_mlt": [{"subitem_source_identifier": "AN00183393", "subitem_source_identifier_type": "NCID"}]}, "item_7_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "03858634", "subitem_source_identifier_type": "ISSN"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Niimura, Sueo"}], "nameIdentifiers": [{"nameIdentifier": "163773", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Yoshihiisa, Yohko"}], "nameIdentifiers": [{"nameIdentifier": "163774", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Takano, Hiroko"}], "nameIdentifiers": [{"nameIdentifier": "163775", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-08-20"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "17_0014.pdf", "filesize": [{"value": "324.1 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 324100.0, "url": {"label": "17_0014.pdf", "url": "https://niigata-u.repo.nii.ac.jp/record/28471/files/17_0014.pdf"}, "version_id": "c00f6a78-6188-4905-89c0-d53717af6cf7"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "denuded porcine oocyte", "subitem_subject_scheme": "Other"}, {"subitem_subject": "nuclear maturation", "subitem_subject_scheme": "Other"}, {"subitem_subject": "cytoplasmic maturation", "subitem_subject_scheme": "Other"}, {"subitem_subject": "steroid", "subitem_subject_scheme": "Other"}, {"subitem_subject": "cortical granule", "subitem_subject_scheme": "Other"}, {"subitem_subject": "ブタ裸化卵母細胞", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "核成熟", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "細胞質成熟", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "ステロイド", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "表層粒", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Effects of Steroids on Maturation of Denuded Porcine Oocytes", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Effects of Steroids on Maturation of Denuded Porcine Oocytes"}, {"subitem_title": "Effects of Steroids on Maturation of Denuded Porcine Oocytes", "subitem_title_language": "en"}]}, "item_type_id": "7", "owner": "1", "path": ["456", "1607"], "permalink_uri": "http://hdl.handle.net/10191/681", "pubdate": {"attribute_name": "公開日", "attribute_value": "2007-04-18"}, "publish_date": "2007-04-18", "publish_status": "0", "recid": "28471", "relation": {}, "relation_version_is_last": true, "title": ["Effects of Steroids on Maturation of Denuded Porcine Oocytes"], "weko_shared_id": null}