{"_buckets": {"deposit": "31ec3f8f-04d8-4341-abb7-ff3c284dcbdb"}, "_deposit": {"id": "28436", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "28436"}, "status": "published"}, "_oai": {"id": "oai:niigata-u.repo.nii.ac.jp:00028436", "sets": ["456", "1604"]}, "item_7_alternative_title_1": {"attribute_name": "その他のタイトル", "attribute_value_mlt": [{"subitem_alternative_title": "ツニカマイシン処置したマウス卵母細胞の囲卵腔の大きさと多精子侵入の頻度"}]}, "item_7_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2006-08", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "1", "bibliographicPageEnd": "31", "bibliographicPageStart": "27", "bibliographicVolumeNumber": "59", "bibliographic_titles": [{"bibliographic_title": "新潟大学農学部研究報告"}, {"bibliographic_title": "新潟大学農学部研究報告", "bibliographic_titleLang": "en"}]}]}, "item_7_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "The size of the perivitelline space was measured in mouse oocytes matured in TYH medium containing tunicamycin, an inhibitor of glycoprotein synthesis, and the incidence of polyspermy in those following insemination was observed, in order to examine whether the relationship between the incidence of polyspermy and the size of perivitelline space exists in mouse oocytes. The size of perivitelline space in oocytes treated with tunicamycin was 4.5μm, which was significantly smaller than 5.4μm in control oocytes matured in TYH medium. The rate of fertilization after insemination was similar between oocytes matured in TYH medium containing tunicamycin (91.5%) and control oocytes matured in TYH medium (92.2%), while the incidence of polyspermy was significantly higher in oocytes matured in TYH medium containing tunicamycin (79.1%) than in control oocytes matured in TYH medium (57.6%). From these findings, it was clarified that the higher incidence of polyspermy is closely related to the smaller perivitelline space in mouse oocytes.糖タンパク質合成の阻害剤であるツニカマイシンを含むTYH液で培養したマウスの卵丘卵母細胞複合体について、成熟後の卵母細胞の囲卵腔の大きさと媒精後の多精子侵入の頻度を観察し、囲卵腔の大きさと多精子侵入との間に関係があるのか否かを検討した。囲卵腔は、対照のTYH液で培養して成熟させた卵母細胞では5.4μmあったが、ツニカマイシン処置した卵母細胞では有意に小さく、4.5μmであった。一方、媒精後の受精率は、ツニカマイシン処置した卵母細胞では91.5%であり、対照の卵母細胞の92.2%と相違なかったが、多精子侵入率は、ツニカマイシン処置した卵母細胞では79.1%であり、対照の卵母細胞の57.6%に比べて有意に高かった。以上の結果から、囲卵腔が小さい卵母細胞では媒精後の多精子侵入の頻度が高いことが確かめられ、囲卵腔の大きさと多精子侵入の頻度との間には密接な関係のあることがマウスでも確認された。", "subitem_description_type": "Abstract"}]}, "item_7_full_name_3": {"attribute_name": "著者別名", "attribute_value_mlt": [{"nameIdentifiers": [{"nameIdentifier": "163615", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "北川, 時久"}]}, {"nameIdentifiers": [{"nameIdentifier": "163616", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "新村, 末雄"}]}]}, "item_7_publisher_7": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "新潟大学農学部"}]}, "item_7_select_19": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_7_source_id_11": {"attribute_name": "書誌レコードID", "attribute_value_mlt": [{"subitem_source_identifier": "AN00183393", "subitem_source_identifier_type": "NCID"}]}, "item_7_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "03858634", "subitem_source_identifier_type": "ISSN"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Kitagawa, Tokihisa"}], "nameIdentifiers": [{"nameIdentifier": "163613", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Niimura, Sueo"}], "nameIdentifiers": [{"nameIdentifier": "163614", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-08-20"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "17_0050.pdf", "filesize": [{"value": "252.3 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 252300.0, "url": {"label": "17_0050.pdf", "url": "https://niigata-u.repo.nii.ac.jp/record/28436/files/17_0050.pdf"}, "version_id": "7a01a759-0b51-44e4-b75e-c7bc4a6b673c"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "mouse oocyte", "subitem_subject_scheme": "Other"}, {"subitem_subject": "sizeof perivitelline space", "subitem_subject_scheme": "Other"}, {"subitem_subject": "incidence of polyspermy", "subitem_subject_scheme": "Other"}, {"subitem_subject": "マウス卵母細胞", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "囲卵腔の大きさ", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "多精子侵入の頻度", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Size of Perivitelline Space and Incidence of Polyspermy in Mouse Oocytes Treated with Tunicamycin", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Size of Perivitelline Space and Incidence of Polyspermy in Mouse Oocytes Treated with Tunicamycin"}, {"subitem_title": "Size of Perivitelline Space and Incidence of Polyspermy in Mouse Oocytes Treated with Tunicamycin", "subitem_title_language": "en"}]}, "item_type_id": "7", "owner": "1", "path": ["456", "1604"], "permalink_uri": "http://hdl.handle.net/10191/693", "pubdate": {"attribute_name": "公開日", "attribute_value": "2007-04-18"}, "publish_date": "2007-04-18", "publish_status": "0", "recid": "28436", "relation": {}, "relation_version_is_last": true, "title": ["Size of Perivitelline Space and Incidence of Polyspermy in Mouse Oocytes Treated with Tunicamycin"], "weko_shared_id": null}