{"_buckets": {"deposit": "e2deb79d-3e3a-4c4c-98a6-e5fee9056156"}, "_deposit": {"id": "28431", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "28431"}, "status": "published"}, "_oai": {"id": "oai:niigata-u.repo.nii.ac.jp:00028431", "sets": ["456", "1603"]}, "item_7_alternative_title_1": {"attribute_name": "その他のタイトル", "attribute_value_mlt": [{"subitem_alternative_title": "低NaCl 濃度の培養液で培養したマウス卵母細胞の囲卵腔の大きさ"}]}, "item_7_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2007-03", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "2", "bibliographicPageEnd": "106", "bibliographicPageStart": "100", "bibliographicVolumeNumber": "59", "bibliographic_titles": [{"bibliographic_title": "新潟大学農学部研究報告"}, {"bibliographic_title": "新潟大学農学部研究報告", "bibliographic_titleLang": "en"}]}]}, "item_7_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "The size of each part of mouse oocytes cultured in TYH medium and modified TYH medium with decreased concentration of NaCl was measured to investigate how the size of the perivitelline space changed as oocytes matured, and the reason(s) the size of the perivitelline space changed. The incidence of polyspermy in oocytes matured in modified TYH medium with decreased concentration of NaCl was also examined, in order to ascertain a relationship between the size of perivitelline space and the incidence of polyspermy.When immature oocytes were cultured in modified TYH medium containing NaCl at 95.82, 82.15 or 68.49 mM, 92.6 to 100.0 % of the oocytes matured to the metaphase II stage, showing no difference from the maturation rate (93.8 %) of control oocytes cultured in TYH medium. The maturation rate of oocytes cultured in modified TYH medium with NaCl at 44.50 mM was 0 %, which was significantly lower than that of the control oocytes. The size of perivitelline space in oocytes matured in modified TYH medium containing NaCl at 95.82 or 82.15 mM was 5.34 and 5.40 μm, respectively, showing no difference from the 5.40 μm of control oocytes. On the other hand, the size of perivitelline space of oocytes matured in modified TYH medium containing NaCl at 68.49 mM was 6.12 μm, which was significantly larger than those of the oocytes matured in TYH medium and modified TYH media containing NaCl at 95.82 and 82.15 mM. The size of perivitelline space in oocytes immediately after collection was 0.28 μm. In oocytes cultured in TYH medium and modified TYH medium containing NaCl at 68.49 mM (low NaCl-TYH medium), perivitelline space enlarged as the time of culture was prolonged, even in the use of either medium examined. The perivitelline space in oocytes cultured in low NaCl-TYH medium for 14 hrs was 6.12 μm, which was significantly larger than 5.40 μm of control oocytes cultured in TYH medium. From the measurements of each part of oocytes during culture, it was suggested that the enlargement in the perivitelline space following treatment with low NaCl was attributed to the increase in inner diameter of their zonae pellucidae. The rate of fertilization after insemination was similar between oocytes matured in low NaCl-TYH medium (92.7 %) and control oocytes matured in TYH medium (92.2 %), while the incidence of polyspermy was significantly lower in oocytes matured in low NaCl-TYH medium (40.4 %) than in control oocytes (57.6 %). From these findings, it was confirmed that there is a close relationship between the size of the perivitelline space and the incidence of polyspermy in mouse oocytes.", "subitem_description_type": "Abstract"}, {"subitem_description": "NaClの濃度を低くした低NaCl-TYH培養液で培養したマウス卵母細胞について、各部位の大きさを計測して通常のTYH培養液で培養した卵母細胞のものと比較し、低NaCl処置した卵母細胞の囲卵腔の大きさが変化する原因について検討した。併せて、低NaCl-TYH培養液で成熟させたマウス卵母細胞の媒精後の多精子侵入の頻度を調べた。胞状卵胞から採取した卵母細胞を、NaClの濃度を95.82、82.15および68.49mMに低下した修正TYH培養液で14時間培養すると、92.6ないし100%が成熟し、成熟率は対照のTYH培養液(NaCl濃度は119.37mM)で培養した卵母細胞の93.8%と相違なかったが、NaClの濃度を44.50mMに低下した培養液で培養したものでは成熟したものはみられなかった。一方、囲卵腔の大きさは、NaClの濃度を95.82および82.15mMに低下した培養液で培養した卵母細胞では5.34および5.40μmであり、対照の卵母細胞の5.40μmと相違なかったが、NaClの濃度を68.49mMに低下した培養液で培養したものでは有意に大きく、6.12μmになった。囲卵腔は、採取直後の卵母細胞では0.28μmあったが、TYH培養液およびNaClの濃度を68.49mMに低下した修正TYH培養液(低NaCl-TYH培養液)で培養したものでは、いずれも培養時間の経過に伴って徐々に拡大した。なお、培養後2、4、6、8および14時間では、囲卵腔は、TYH培養液で培養した卵母細胞に比べ、低NaCl-TYH培養液で培養した卵母細胞で有意に大きかった。透明帯の内径は、採取直後の卵母細胞とTYH培養液でいずれの時間培養した卵母細胞との間で大きく変化しなかったが、低NaCl-TYH培養液で培養した卵母細胞では77.67ないし79.98μmとなり、すべての培養時間で、TYH培養液で培養したものに比べて有意に大きかった。卵母細胞の直径は、TYH培養液および低NaCl-TYH培養液で培養したものの両者で、採取直後の卵母細胞のものに比べて有意に小さくなるとともに、培養後4、6、8、10、12および14時間では、TYH培養液で培養したものに比べて低NaCl-TYH培養液で培養したもので有意に大きかった。透明帯の厚さは、採取直後の卵母細胞とTYH培養液で培養した卵母細胞との間で相違なかった。一方、低NaCl-TYH培養液で培養した卵母細胞の透明帯は、7.58ないし8.70μmあり、培養後14時間ではTYH培養液で培養したものと相違なかったが、培養後2、4、6、8、10および12時間ではTYH培養液で培養したものに比べて有意に薄かった。これらのことから、低NaCl-TYH培養液で培養した卵母細胞で囲卵腔が大きくなるのは、透明帯の内径が大きくなるためであると考えられた。一方、低NaCl-TYH培養液で成熟させた卵母細胞に媒精したところ、受精率は92.7%であり、対照のTYH培養液で成熟させた卵母細胞の92.2%と相違なかったが、多精子侵入率は40.4%であり、対照の卵母細胞の57.6%に比べて有意に低かった。従って、低NaCl濃度の培養液で成熟させて囲卵腔を有意に拡大すると、媒精後の多精子侵入の頻度が有意に低下することが確かめられ、卵母細胞の囲卵腔の大きさと媒精後の多精子侵入の頻度との間には密接な関係のあることが確かめられた。", "subitem_description_type": "Abstract"}]}, "item_7_full_name_3": {"attribute_name": "著者別名", "attribute_value_mlt": [{"nameIdentifiers": [{"nameIdentifier": "163591", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "新村, 末雄"}]}, {"nameIdentifiers": [{"nameIdentifier": "163592", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "上野, 紗也香"}]}, {"nameIdentifiers": [{"nameIdentifier": "163593", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "北川, 時久"}]}, {"nameIdentifiers": [{"nameIdentifier": "163594", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "佐藤, 佳乃子"}]}]}, "item_7_publisher_7": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "新潟大学農学部"}]}, "item_7_select_19": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_7_source_id_11": {"attribute_name": "書誌レコードID", "attribute_value_mlt": [{"subitem_source_identifier": "AN00183393", "subitem_source_identifier_type": "NCID"}]}, "item_7_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "03858634", "subitem_source_identifier_type": "ISSN"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Niimura, Sueo"}], "nameIdentifiers": [{"nameIdentifier": "163587", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ueno, Sayaka"}], "nameIdentifiers": [{"nameIdentifier": "163588", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kitagawa, Tokihisa"}], "nameIdentifiers": [{"nameIdentifier": "163589", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Sato, Kanoko"}], "nameIdentifiers": [{"nameIdentifier": "163590", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-08-20"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "100-106.pdf", "filesize": [{"value": "322.3 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 322300.0, "url": {"label": "100-106.pdf", "url": "https://niigata-u.repo.nii.ac.jp/record/28431/files/100-106.pdf"}, "version_id": "19ef38a3-db47-4b08-aaee-ec60a9fdb4ed"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "culture medium with decreased concentration of NaCl", "subitem_subject_scheme": "Other"}, {"subitem_subject": "incidence of polyspermy", "subitem_subject_scheme": "Other"}, {"subitem_subject": "mouse oocyte", "subitem_subject_scheme": "Other"}, {"subitem_subject": "size of perivitelline space", "subitem_subject_scheme": "Other"}, {"subitem_subject": "囲卵腔の大きさ", "subitem_subject_scheme": "Other"}, {"subitem_subject": "多精子侵入の頻度", "subitem_subject_scheme": "Other"}, {"subitem_subject": "低NaCl濃度培養液", "subitem_subject_scheme": "Other"}, {"subitem_subject": "マウス卵母細胞", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "The Size of Perivitelline Space in Mouse Oocytes Cultured in the Medium with Decreased Concentration of NaCl", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "The Size of Perivitelline Space in Mouse Oocytes Cultured in the Medium with Decreased Concentration of NaCl"}, {"subitem_title": "The Size of Perivitelline Space in Mouse Oocytes Cultured in the Medium with Decreased Concentration of NaCl", "subitem_title_language": "en"}]}, "item_type_id": "7", "owner": "1", "path": ["456", "1603"], "permalink_uri": "http://hdl.handle.net/10191/4857", "pubdate": {"attribute_name": "公開日", "attribute_value": "2007-05-29"}, "publish_date": "2007-05-29", "publish_status": "0", "recid": "28431", "relation": {}, "relation_version_is_last": true, "title": ["The Size of Perivitelline Space in Mouse Oocytes Cultured in the Medium with Decreased Concentration of NaCl"], "weko_shared_id": null}