{"_buckets": {"deposit": "5c055ac5-b18a-477f-b65f-4715a0da4999"}, "_deposit": {"id": "28422", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "28422"}, "status": "published"}, "_oai": {"id": "oai:niigata-u.repo.nii.ac.jp:00028422"}, "item_7_alternative_title_1": {"attribute_name": "\u305d\u306e\u4ed6\u306e\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_alternative_title": "Brassica rapa \u57f9\u990a\u6839\u306e\u6839\u3053\u3076\u75c5\u75c5\u539f\u83ccPlasmodiophora brassicae \u306b\u5bfe\u3059\u308b\u62b5\u6297\u53cd\u5fdc\u306e\u30d7\u30ed\u30c6\u30aa\u30fc\u30e0\u89e3\u6790"}]}, "item_7_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2007-08", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "1", "bibliographicPageEnd": "71", "bibliographicPageStart": "67", "bibliographicVolumeNumber": "60", "bibliographic_titles": [{"bibliographic_title": "\u65b0\u6f5f\u5927\u5b66\u8fb2\u5b66\u90e8\u7814\u7a76\u5831\u544a"}, {"bibliographic_title": "\u65b0\u6f5f\u5927\u5b66\u8fb2\u5b66\u90e8\u7814\u7a76\u5831\u544a", "bibliographic_titleLang": "en"}]}]}, "item_7_description_4": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "Comparative proteome analysis of the resistance responses in cultured roots of Brassica rapa (turnip) on the treatment with resting spore of Plasmodiophora brassicae, which is the causal agent of clubroot disease, was performed by 2-D PAGE. Using cultured roots from a susceptible and a resistant cultivar, 251 protein spots were clearly observed in 2D-PAGE and protein profiles were compared between resistant and susceptible roots as well as spore-treated and non-treated roots. In comparison between resistant and susceptible non-treated roots, 11 and 8 spots were significantly higher in intensity in resistant and susceptible roots, respectively. The treatment with resting spores of P. brassicae affected protein profiles of 2D-PAGE in a different manner between susceptible and resistant roots. In spore-treated susceptible roots, concentration of 6 protein spots increased but 7 were reduced compared to those in untreated control. These proteins are possibly involved in infectious events necessary for root hair infection or for defense responses in non-pathogen-specific stresses. On the other hand, in the spore treated resistant roots, 10 and 13 spots were up- and down-regulated, respectively. The protein profile of the resistant roots treated with the spore was clearly different from that of the susceptible ones, suggested that the fluctuated proteins of resistant roots should include the one(s) specific to resistant responses. \u6839\u3053\u3076\u75c5\u83cc\u611f\u67d3\u521d\u671f\u53cd\u5fdc\u3092\u89e3\u660e\u3059\u308b\u305f\u3081\u3001\u6839\u3053\u3076\u75c5\u83cc\u611f\u53d7\u6027\u3068\u62b5\u6297\u6027\u306e\uff12\u7cfb\u7d71\u306e\u30ab\u30d6\u304b\u3089\u8a98\u5c0e\u3057\u305f\u57f9\u990a\u6839\u306b\u6839\u3053\u3076\u75c5\u83ccPlasmodiophora brassicae \u306e\u80de\u5b50\u3092\u51e6\u7406\u3057\u3001\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u6d88\u9577\u30922-D PAGE 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"file_order": 0, "filename": "1_0010.pdf", "filesize": [{"value": "141.1 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 141100.0, "url": {"label": "1_0010.pdf", "url": "https://niigata-u.repo.nii.ac.jp/record/28422/files/1_0010.pdf"}, "version_id": "7f2e841b-8719-4089-9653-c0cd1388d253"}]}, "item_keyword": {"attribute_name": "\u30ad\u30fc\u30ef\u30fc\u30c9", "attribute_value_mlt": [{"subitem_subject": "Brassica rapa", "subitem_subject_scheme": "Other"}, {"subitem_subject": "clubroot resistance", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Plasmodiophora brassicae", "subitem_subject_scheme": "Other"}, {"subitem_subject": "root culture", "subitem_subject_scheme": "Other"}, {"subitem_subject": "2-D PAGE", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Brassica rapa", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Plasmodiophora brassicae", "subitem_subject_scheme": "Other"}, {"subitem_subject": "\u4e8c\u6b21\u5143\u96fb\u6c17\u6cf3\u52d5", "subitem_subject_scheme": "Other"}, {"subitem_subject": "\u6839\u3053\u3076\u75c5\u62b5\u6297\u6027", "subitem_subject_scheme": "Other"}, {"subitem_subject": "\u57f9\u990a\u6839", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Comparative Proteomic Analysis of the Resistant Response in Brassica rapa Root Culture to the Clubroot Disease Agent Plasmodiophora brassicae", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "Comparative Proteomic Analysis of the Resistant Response in Brassica rapa Root Culture to the Clubroot Disease Agent Plasmodiophora brassicae"}, {"subitem_title": "Comparative Proteomic Analysis of the Resistant Response in Brassica rapa Root Culture to the Clubroot Disease Agent Plasmodiophora brassicae", "subitem_title_language": "en"}]}, "item_type_id": "7", "owner": "1", "path": ["453/456", "485/872/1583/1602"], "permalink_uri": "http://hdl.handle.net/10191/5040", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_value": "2007-10-12"}, "publish_date": "2007-10-12", "publish_status": "0", "recid": "28422", "relation": {}, "relation_version_is_last": true, "title": ["Comparative Proteomic Analysis of the Resistant Response in Brassica rapa Root Culture to the Clubroot Disease Agent Plasmodiophora brassicae"], "weko_shared_id": null}