{"_buckets": {"deposit": "30b33bdc-1dfa-474d-b103-1d0fbfd0785e"}, "_deposit": {"id": "28420", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "28420"}, "status": "published"}, "_oai": {"id": "oai:niigata-u.repo.nii.ac.jp:00028420"}, "item_7_alternative_title_1": {"attribute_name": "\u305d\u306e\u4ed6\u306e\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_alternative_title": "\u30a4\u30f3\u30c9\u30fc\u30eb-3-\u30a2\u30bb\u30c8\u30a2\u30df\u30c9\u3092\u30a4\u30f3\u30c9\u30fc\u30eb-3-\u9162\u9178\u306b\u5909\u63db\u3059\u308b\u9175\u7d20\u30a2\u30df\u30c0\u30fc\u30bc\u306e\u30ab\u30d6\u6839\u3053\u3076\u75c5\u306b\u304a\u3051\u308b\u5f79\u5272\u306e\u8a55\u4fa1"}]}, "item_7_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2007-08", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "1", "bibliographicPageEnd": "60", "bibliographicPageStart": "53", "bibliographicVolumeNumber": "60", "bibliographic_titles": [{"bibliographic_title": "\u65b0\u6f5f\u5927\u5b66\u8fb2\u5b66\u90e8\u7814\u7a76\u5831\u544a"}, {"bibliographic_title": "\u65b0\u6f5f\u5927\u5b66\u8fb2\u5b66\u90e8\u7814\u7a76\u5831\u544a", "bibliographic_titleLang": "en"}]}]}, "item_7_description_4": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "Amidase was investigated if it was one of auxin-producing enzymes of Brassica rapa. We found amidase activity to convert indole-3-acetamide to indole-3-acetic acid in soluble protein extracts from B. rapa. Optimum condition for the enzyme activity was searched and two optimum temperatures were obtained at 45 and 55\u2103. Search for the heat stability of the enzyme strongly suggested that the two summits of the optimum temperatures were resulted from occurrence of variant amidases showing different temperature stabilities. The possibility of the presence of several amidase isoforms was supported by the following two observations, i.e., firstly, amidase activities at 45 and 55\u2103 had different pH optimums, 8.5 and 7.5, respectively. Secondly, the enzyme activities at the two temperatures were differentially fluctuated during the vegetative growth of turnip and clubroot development. Fluctuation of the amidase activity and IAA contents observed in various turnip tissues such as healthy turnip leaf, hypocotyl and roots suggested that the enzyme had a constitutive role for keeping IAA homeostasis in those tissues. Interestingly, the amidase in turnip was shown to have high activity in hypocotyl and root rather than leaf, unlike Arabidopsis one. Changes of the enzyme activity during development of B. rapa was analyzed using the tissues of clubrootdiseased turnips. The activities fluctuated differently from each other in the temperature at 45 and 55\u2103 in infected tissues. In addition, activity at 45\u2103 was specifically enhanced in a later phase of clubroot development, whereas one at 55\u2103 increased only in an early phase in the infected root tissues. These results indicated that the amidase played an important role in turnip growth and clubroot development. \u6839\u3053\u3076\u75c5\u83ccPlasmodiophora brassicae \u306b\u3088\u308b\u6839\u3053\u3076\u5f62\u6210\u306e\u5206\u5b50\u6a5f\u69cb\u89e3\u660e\u3092\u76ee\u7684\u306b\u30aa\u30fc\u30ad\u30b7\u30f3\u5408\u6210\u9175\u7d20\u306e1\u3064\u30a2\u30df\u30c0\u30fc\u30bc\u6d3b\u6027\u3092\u3001\u30ab\u30d6\u7d44\u7e54\u304b\u3089\u53ef\u6eb6\u6027\u7c97\u9175\u7d20\u753b\u5206\u3092\u8abf\u88fd\u3057\u6e2c\u5b9a\u3057\u305f\u3002\u6d3b\u6027\u6700\u9069\u6e29\u5ea6\u306f45\u53ca\u307355\u2103\u3067\u3042\u308a\u3001\u6700\u9069pH \u306f\u3001\u53cd\u5fdc\u6e29\u5ea645\u2103\u3067pH 8.5\u300155\u2103\u3067pH 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[{"creatorName": "Okazaki, Keiichi"}], "nameIdentifiers": [{"nameIdentifier": "163509", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Itoh, Kimiko"}], "nameIdentifiers": [{"nameIdentifier": "163510", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Mitsui, Toshiaki"}], "nameIdentifiers": [{"nameIdentifier": "163511", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Hori, Hidetaka"}], "nameIdentifiers": [{"nameIdentifier": "163512", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-08-20"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "1_0008.pdf", "filesize": [{"value": "212.5 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", 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{"subitem_subject": "\u30a4\u30f3\u30c9\u30fc\u30eb-3-\u30a2\u30bb\u30c8\u30a2\u30df\u30c9", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Evaluation of Roles of Amidase Which Converts Indole-3-Acetamide to Indole-3-Acetic Acid, in Formation of Clubroot in Turnip", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "Evaluation of Roles of Amidase Which Converts Indole-3-Acetamide to Indole-3-Acetic Acid, in Formation of Clubroot in Turnip"}, {"subitem_title": "Evaluation of Roles of Amidase Which Converts Indole-3-Acetamide to Indole-3-Acetic Acid, in Formation of Clubroot in Turnip", "subitem_title_language": "en"}]}, "item_type_id": "7", "owner": "1", "path": ["453/456", "485/872/1583/1602"], "permalink_uri": "http://hdl.handle.net/10191/5038", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_value": "2007-10-12"}, "publish_date": "2007-10-12", "publish_status": "0", "recid": "28420", "relation": {}, "relation_version_is_last": true, "title": ["Evaluation of Roles of Amidase Which Converts Indole-3-Acetamide to Indole-3-Acetic Acid, in Formation of Clubroot in Turnip"], "weko_shared_id": null}