{"_buckets": {"deposit": "2272721e-afce-41b0-be9b-4374d76bf0b7"}, "_deposit": {"id": "28408", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "28408"}, "status": "published"}, "_oai": {"id": "oai:niigata-u.repo.nii.ac.jp:00028408"}, "item_7_alternative_title_1": {"attribute_name": "\u305d\u306e\u4ed6\u306e\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_alternative_title": "The Amount and the Role of Hyaluronic Acid Secreted by Mouse Cumulus-Oocyte Complexes"}]}, "item_7_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2008-03", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "2", "bibliographicPageEnd": "133", "bibliographicPageStart": "129", "bibliographicVolumeNumber": "60", "bibliographic_titles": [{"bibliographic_title": "\u65b0\u6f5f\u5927\u5b66\u8fb2\u5b66\u90e8\u7814\u7a76\u5831\u544a"}, {"bibliographic_title": "\u65b0\u6f5f\u5927\u5b66\u8fb2\u5b66\u90e8\u7814\u7a76\u5831\u544a", "bibliographic_titleLang": "en"}]}]}, "item_7_description_4": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "\u30de\u30a6\u30b9\u306e\u5375\u4e18\u30fb\u5375\u6bcd\u7d30\u80de\u8907\u5408\u4f53(COC)\u304b\u3089\u5206\u6ccc\u3055\u308c\u308b\u30d2\u30a2\u30eb\u30ed\u30f3\u9178\u306e\u91cf\u3092\u7af6\u5408ELISA\u6cd5\u3067\u691c\u51fa\u3057\u305f\u3002\u307e\u305f\u3001\u30d2\u30a2\u30eb\u30ed\u30f3\u9178\u5408\u6210\u3092\u963b\u5bb3\u3057\u305fCOC\u306b\u3064\u3044\u3066\u3001\u30d2\u30a2\u30eb\u30ed\u30f3\u9178\u306e\u5206\u6ccc\u91cf\u3092\u6e2c\u5b9a\u3059\u308b\u3068\u3068\u3082\u306b\u3001\u5375\u6bcd\u7d30\u80de\u306b\u3064\u3044\u3066\u306f\u56f2\u5375\u8154\u306e\u5927\u304d\u3055\u3068\u5a92\u7cbe\u5f8c\u306e\u591a\u7cbe\u5b50\u53d7\u7cbe\u306e\u983b\u5ea6\u3092\u89b3\u5bdf\u3057\u3001COC\u304b\u3089\u5206\u6ccc\u3055\u308c\u308b\u30d2\u30a2\u30eb\u30ed\u30f3\u9178\u306e\u56f2\u5375\u8154\u306e\u5927\u304d\u3055\u306b\u679c\u305f\u3059\u5f79\u5272\u304a\u3088\u3073\u56f2\u5375\u8154\u306e\u5927\u304d\u3055\u3068\u591a\u7cbe\u5b50\u53d7\u7cbe\u306e\u983b\u5ea6\u3068\u306e\u95a2\u4fc2\u306e\u6709\u7121\u3092\u691c\u8a0e\u3057\u305f\u3002\u30d2\u30a2\u30eb\u30ed\u30f3\u9178\u306f\u3001\u80de\u72b6\u5375\u80de\u304b\u3089\u63a1\u53d6\u76f4\u5f8c\u306eCOC\u304b\u3089\u306f\u691c\u51fa\u3055\u308c\u306a\u304b\u3063\u305f\u304c\u3001\u57f9\u990a\u5f8c14\u6642\u9593\u306eCOC\u3067\u306f1\u500b\u3042\u305f\u308a8.40ng\u691c\u51fa\u3055\u308c\u305f\u3002\u307e\u305f\u3001\u30d2\u30a2\u30eb\u30ed\u30f3\u9178\u5408\u6210\u3092\u963b\u5bb3\u3059\u308b\u4f5c\u7528\u306e\u3042\u308b4-\u30e1\u30c1\u30eb\u30a6\u30f3\u30d9\u30ea\u30d5\u30a7\u30ed\u30f3(MU)\u3067\u51e6\u7f6e\u3057\u305fCOC\u3067\u306f\u30011\u500b\u3042\u305f\u308a3.52ng\u306e\u30d2\u30a2\u30eb\u30ed\u30f3\u9178\u304c\u691c\u51fa\u3055\u308c\u305f\u304c\u3001\u3053\u306e\u91cf\u306f\u5bfe\u7167\u306eCOC\u306e6.54ng\u306b\u6bd4\u3079\u3066\u6709\u610f\u306b\u5c11\u306a\u304b\u3063\u305f\u3002\u4e00\u65b9\u3001\u56f2\u5375\u8154\u306f\u3001\u5bfe\u7167\u306eCOC\u306b\u304a\u3051\u308b\u5375\u6bcd\u7d30\u80de\u3067\u306f\u5e73\u5747\u30675.48mm\u3042\u3063\u305f\u304c\u3001MU\u51e6\u7f6e\u3057\u305fCOC\u306b\u304a\u3051\u308b\u5375\u6bcd\u7d30\u80de\u3067\u306f\u6709\u610f\u306b\u5c0f\u3055\u304f\u30014.36mm\u3067\u3042\u3063\u305f\u3002\u307e\u305f\u3001\u5a92\u7cbe\u5f8c\u306e\u53d7\u7cbe\u7387\u306f\u3001MU\u51e6\u7f6e\u3057\u305fCOC\u306b\u304a\u3051\u308b\u5375\u6bcd\u7d30\u80de(82.2%)\u3068\u5bfe\u7167\u306eCOC\u306b\u304a\u3051\u308b\u5375\u6bcd\u7d30\u80de(88.6%)\u3068\u306e\u9593\u3067\u76f8\u9055\u306a\u304b\u3063\u305f\u304c\u3001\u591a\u7cbe\u5b50\u53d7\u7cbe\u306e\u983b\u5ea6\u306f\u3001MU\u51e6\u7f6e\u3057\u305fCOC\u306b\u304a\u3051\u308b\u5375\u6bcd\u7d30\u80de\u3067\u306f37.8%\u3067\u3042\u308a\u3001\u5bfe\u7167\u306eCOC\u306b\u304a\u3051\u308b\u5375\u6bcd\u7d30\u80de\u306e16.1%\u306b\u6bd4\u3079\u3066\u6709\u610f\u306b\u9ad8\u304b\u3063\u305f\u3002\u4ee5\u4e0a\u306e\u7d50\u679c\u304b\u3089\u3001COC\u304b\u3089\u5206\u6ccc\u3055\u308c\u308b\u30d2\u30a2\u30eb\u30ed\u30f3\u9178\u306f\u3001\u5375\u6bcd\u7d30\u80de\u306e\u56f2\u5375\u8154\u306e\u62e1\u5927\u306b\u95a2\u4fc2\u3057\u3066\u3044\u308b\u3053\u3068\u3001\u307e\u305f\u3001\u5375\u6bcd\u7d30\u80de\u306e\u56f2\u5375\u8154\u306e\u5927\u304d\u3055\u3068\u591a\u7cbe\u5b50\u53d7\u7cbe\u306e\u983b\u5ea6\u3068\u306e\u9593\u306b\u306f\u95a2\u4fc2\u306e\u3042\u308b\u3053\u3068\u304c\u305d\u308c\u305e\u308c\u8003\u3048\u3089\u308c\u305f\u3002Using competitive enzyme linked immunosorbent assay (ELISA), the amount of hyaluronic acid secreted by mouse cumulus-oocyte complexes (COCs) was examined. The size of perivitelline space and the incidence of polyspermy in mouse oocytes of COCs treated with 4-methylumbelliferone (MU), an inhibitor of hyaluronic acid synthase, were observed, in order to examine the role of hyaluronic acid secreted by COCs in the size of perivitelline space of oocytes, and the relationship between the size of perivitelline space and the incidence of polyspermy of oocytes following insemination. No hyaluronic acid was detected in COCs collected from antral follicles, while 8.40ng hyaluronic acid was demonstrated in a COC cultured for 14 hrs. The amount of hyaluronic acid in a COC cultured for 14 hrs with MU was 3.52ng, which was significantly smaller than 6.54ng in a control COC. The mean size of perivitelline space of oocytes was significantly smaller in MU-treated COCs (4.36mm) than in control COCs (5.48mm). The fertilization rate of oocytes in MU-treated COCs after insemination (82.2%) did not differ from that in control COCs (88.6%). However, the incidence of polyspermy in oocytes was significantly higher in MU-treated COCs (37.8%) than in control COCs (16.1%). From these results, it was suggested that hyaluronic acid secreted from COCs plays a role in enlargement of perivitelline space of mouse oocytes, and that there is a relationship between the size of perivitelline space and the incidence of polyspermy in the mouse.", "subitem_description_type": "Abstract"}]}, "item_7_full_name_3": {"attribute_name": "\u8457\u8005\u5225\u540d", "attribute_value_mlt": [{"nameIdentifiers": [{"nameIdentifier": "163429", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "Ueno, Sayaka"}]}, {"nameIdentifiers": [{"nameIdentifier": "163430", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "Niimura, Sueo"}]}]}, "item_7_publisher_7": {"attribute_name": "\u51fa\u7248\u8005", "attribute_value_mlt": [{"subitem_publisher": "\u65b0\u6f5f\u5927\u5b66\u8fb2\u5b66\u90e8"}]}, "item_7_select_19": {"attribute_name": "\u8457\u8005\u7248\u30d5\u30e9\u30b0", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_7_source_id_11": {"attribute_name": "\u66f8\u8a8c\u30ec\u30b3\u30fc\u30c9ID", "attribute_value_mlt": [{"subitem_source_identifier": "AN00183393", "subitem_source_identifier_type": "NCID"}]}, "item_7_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "03858634", "subitem_source_identifier_type": "ISSN"}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "\u4e0a\u91ce, \u7d17\u4e5f\u9999"}], "nameIdentifiers": [{"nameIdentifier": "163427", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "\u65b0\u6751, \u672b\u96c4"}], "nameIdentifiers": [{"nameIdentifier": "163428", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-08-20"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "129-133.pdf", "filesize": [{"value": "783.7 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 783700.0, "url": {"label": "129-133.pdf", "url": "https://niigata-u.repo.nii.ac.jp/record/28408/files/129-133.pdf"}, "version_id": "a9f74df2-b4fe-416b-a3b3-7b6e840b037d"}]}, "item_keyword": {"attribute_name": "\u30ad\u30fc\u30ef\u30fc\u30c9", "attribute_value_mlt": [{"subitem_subject": "\u30de\u30a6\u30b9", "subitem_subject_scheme": "Other"}, {"subitem_subject": "\u5375\u4e18\u30fb\u5375\u6bcd\u7d30\u80de\u8907\u5408\u4f53", "subitem_subject_scheme": "Other"}, {"subitem_subject": "\u30d2\u30a2\u30eb\u30ed\u30f3\u9178", "subitem_subject_scheme": "Other"}, {"subitem_subject": "\u56f2\u5375\u8154", "subitem_subject_scheme": "Other"}, {"subitem_subject": "\u591a\u7cbe\u5b50\u53d7\u7cbe", "subitem_subject_scheme": "Other"}, {"subitem_subject": "mouse", "subitem_subject_scheme": "Other"}, {"subitem_subject": "cumulus-oocyte complex", "subitem_subject_scheme": "Other"}, {"subitem_subject": "hyaluronic acid", "subitem_subject_scheme": "Other"}, {"subitem_subject": "perivitelline space", "subitem_subject_scheme": "Other"}, {"subitem_subject": "polyspermy", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "jpn"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "\u30de\u30a6\u30b9\u306e\u5375\u4e18\u30fb\u5375\u6bcd\u7d30\u80de\u8907\u5408\u4f53\u304b\u3089\u5206\u6ccc\u3055\u308c\u308b\u30d2\u30a2\u30eb\u30ed\u30f3\u9178\u306e\u91cf\u3068\u5f79\u5272", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "\u30de\u30a6\u30b9\u306e\u5375\u4e18\u30fb\u5375\u6bcd\u7d30\u80de\u8907\u5408\u4f53\u304b\u3089\u5206\u6ccc\u3055\u308c\u308b\u30d2\u30a2\u30eb\u30ed\u30f3\u9178\u306e\u91cf\u3068\u5f79\u5272"}, {"subitem_title": "\u30de\u30a6\u30b9\u306e\u5375\u4e18\u30fb\u5375\u6bcd\u7d30\u80de\u8907\u5408\u4f53\u304b\u3089\u5206\u6ccc\u3055\u308c\u308b\u30d2\u30a2\u30eb\u30ed\u30f3\u9178\u306e\u91cf\u3068\u5f79\u5272", "subitem_title_language": "en"}]}, "item_type_id": "7", "owner": "1", "path": ["453/456", "485/872/1583/1601"], "permalink_uri": "http://hdl.handle.net/10191/6423", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_value": "2008-05-02"}, "publish_date": "2008-05-02", "publish_status": "0", "recid": "28408", "relation": {}, "relation_version_is_last": true, "title": ["\u30de\u30a6\u30b9\u306e\u5375\u4e18\u30fb\u5375\u6bcd\u7d30\u80de\u8907\u5408\u4f53\u304b\u3089\u5206\u6ccc\u3055\u308c\u308b\u30d2\u30a2\u30eb\u30ed\u30f3\u9178\u306e\u91cf\u3068\u5f79\u5272"], "weko_shared_id": null}