{"_buckets": {"deposit": "6ba3027b-bcf2-42ba-acc2-da2aa057a9cf"}, "_deposit": {"id": "28407", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "28407"}, "status": "published"}, "_oai": {"id": "oai:niigata-u.repo.nii.ac.jp:00028407"}, "item_7_alternative_title_1": {"attribute_name": "\u305d\u306e\u4ed6\u306e\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_alternative_title": "IBMX\u304a\u3088\u3073dbcAMP\u3067\u51e6\u7f6e\u3057\u305f\u30d6\u30bf\u5375\u6bcd\u7d30\u80de\u306b\u304a\u3051\u308b\u6838\u306e\u6210\u719f\u3068\u8868\u5c64\u7c92\u306e\u5206\u5e03"}]}, "item_7_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2008-03", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "2", "bibliographicPageEnd": "128", "bibliographicPageStart": "123", "bibliographicVolumeNumber": "60", "bibliographic_titles": [{"bibliographic_title": "\u65b0\u6f5f\u5927\u5b66\u8fb2\u5b66\u90e8\u7814\u7a76\u5831\u544a"}, {"bibliographic_title": "\u65b0\u6f5f\u5927\u5b66\u8fb2\u5b66\u90e8\u7814\u7a76\u5831\u544a", "bibliographic_titleLang": "en"}]}]}, "item_7_description_4": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "We formerly reported in porcine oocytes that cortical granules (CGs) move to the cytoplasm immediately beneath the plasma membrane as nuclear maturation progresses, and that the movement of CGs and the resumption of meiotic division do not occur in those treated with olomoucine. Therefore, we suggested that CGs distribution in the cytoplasm is closely associated with nuclear maturation. However, whether olomoucine directly acts on the cytoplasm to inhibit such change in CGs distribution could not be determined. In the present investigation, the distribution of CGs was lectin-histochemically observed in porcine oocytes treated with IBMX or dbcAMP, which suppresses the nuclear maturation with the mechanism different from olomoucine, and also in porcine oocytes treated with olomoucine and dbcAMP, in order to examine the relationship between nuclear maturation and the distribution of CGs in the cytoplasm. Nuclei of the oocytes cultured for 22 hrs with IBMX, dbcAMP, or olomoucine and dbcAMP were all in the germinal vesicle (GV) stage. The percentages of oocytes in the GV stage were significantly higher in the treated oocytes than in control oocytes. CGs in the oocytes treated with IBMX, dbcAMP, or olomoucine and dbcAMP were distributed in the cortical cytoplasm and also immediately beneath the plasma membrane. The distribution of CGs in such the treated oocytes did not differ from those of control oocytes. From these results, it was suggested that the changes in the distribution of CGs in the cytoplasm with oocyte maturation depend on the cAMP level in their cytoplasm rather than the progression of nuclear maturation.\u4ee5\u524d\u6211\u3005\u306f\u3001\u30d6\u30bf\u5375\u6bcd\u7d30\u80de\u306b\u304a\u3044\u3066\u3001\u8868\u5c64\u7c92\u306f\u6838\u306e\u6210\u719f\u306b\u4f34\u3063\u3066\u7d30\u80de\u819c\u76f4\u4e0b\u306e\u7d30\u80de\u8cea\u306b\u79fb\u52d5\u3059\u308b\u3068\u3068\u3082\u306b\u3001\u6838\u306e\u6210\u719f\u3092\u30aa\u30ed\u30e2\u30a6\u30b7\u30f3\u3067\u51e6\u7f6e\u3057\u3066\u963b\u6b62\u3057\u305f\u5375\u6bcd\u7d30\u80de\u3067\u306f\u3053\u306e\u3088\u3046\u306a\u8868\u5c64\u7c92\u306e\u5206\u5e03\u5909\u5316\u306f\u307f\u3089\u308c\u306a\u304b\u3063\u305f\u3053\u3068\u304b\u3089\u3001\u8868\u5c64\u7c92\u306e\u5206\u5e03\u5909\u5316\u3068\u6838\u306e\u6210\u719f\u3068\u306f\u5bc6\u63a5\u306b\u95a2\u4fc2\u3057\u3066\u3044\u308b\u3053\u3068\u3092\u63a8\u5bdf\u3057\u3066\u5831\u544a\u3057\u305f\u3002\u3057\u304b\u3057\u3001\u30aa\u30ed\u30e2\u30a6\u30b7\u30f3\u304c\u8868\u5c64\u7c92\u306e\u5206\u5e03\u5909\u5316\u306b\u76f4\u63a5\u95a2\u4fc2\u3057\u3066\u3044\u308b\u306e\u304b\u5426\u304b\u306f\u660e\u3089\u304b\u306b\u3067\u304d\u306a\u304b\u3063\u305f\u3002\u305d\u3053\u3067\u3001\u30aa\u30ed\u30e2\u30a6\u30b7\u30f3\u3068\u306f\u7570\u306a\u3063\u305f\u6a5f\u69cb\u3001\u3059\u306a\u308f\u3061\u7d30\u80de\u8cea\u306ecAMP\u30ec\u30d9\u30eb\u3092\u9ad8\u304f\u7dad\u6301\u3059\u308b\u3053\u3068\u306b\u3088\u3063\u3066\u6838\u306e\u6210\u719f\u3092\u6291\u5236\u3059\u308b\u4f5c\u7528\u306e\u3042\u308bIBMX\u3068dbcAMP\u3092\u7528\u3044\u3066\u3001\u30d6\u30bf\u306e\u5375\u6bcd\u7d30\u80de\u3092\u51e6\u7f6e\u3057\u3001\u8868\u5c64\u7c92\u306e\u5206\u5e03\u5909\u5316\u3092\u30ec\u30af\u30c1\u30f3\u7d44\u7e54\u5316\u5b66\u7684\u306b\u89b3\u5bdf\u3057\u305f\u3002IBMX\u3001dbcAMP\u3042\u308b\u3044\u306f\u30aa\u30ed\u30e2\u30a6\u30b7\u30f3\u3068dbcAMP\u306722\u6642\u9593\u51e6\u7f6e\u3057\u305f\u5375\u6bcd\u7d30\u80de\u306b\u304a\u3044\u3066\u3001\u6838\u306f\u3059\u3079\u3066\u304c\u5375\u6838\u80de\u671f\u306b\u3042\u308a\u3001\u3053\u308c\u3089\u306e\u51e6\u7f6e\u306b\u3088\u308a\u6210\u719f\u5206\u88c2\u304c\u6291\u5236\u3055\u308c\u3066\u3044\u308b\u3053\u3068\u304c\u78ba\u8a8d\u3055\u308c\u305f\u3002\u4e00\u65b9\u3001IBMX\u3001dbcAMP\u3042\u308b\u3044\u306f\u30aa\u30ed\u30e2\u30a6\u30b7\u30f3\u3068dbcAMP\u306722\u6642\u9593\u51e6\u7f6e\u3057\u305f\u5375\u6bcd\u7d30\u80de\u306b\u304a\u3044\u3066\u3001\u8868\u5c64\u7c92\u306f\u3001\u7d30\u80de\u8cea\u306e\u8868\u5c64\u304a\u3088\u3073\u7d30\u80de\u819c\u76f4\u4e0b\u306e\u7d30\u80de\u8cea\u306b\u5206\u5e03\u3057\u3066\u304a\u308a\u3001\u5bfe\u7167\u306e\u5375\u6bcd\u7d30\u80de\u306b\u304a\u3051\u308b\u5206\u5e03\u3068\u76f8\u9055\u306a\u304b\u3063\u305f\u3002\u4ee5\u4e0a\u306e\u7d50\u679c\u304b\u3089\u3001\u7d30\u80de\u8cea\u306b\u304a\u3051\u308b\u8868\u5c64\u7c92\u306e\u5206\u5e03\u306f\u3001\u6838\u306e\u6210\u719f\u306e\u9032\u884c\u3088\u308a\u3082\u3001\u7d30\u80de\u8cea\u306ecAMP\u306e\u91cf\u306b\u4f9d\u5b58\u3057\u3066\u5909\u5316\u3059\u308b\u3053\u3068\u304c\u63a8\u5bdf\u3055\u308c\u305f\u3002", "subitem_description_type": "Abstract"}]}, "item_7_full_name_3": {"attribute_name": "\u8457\u8005\u5225\u540d", "attribute_value_mlt": [{"nameIdentifiers": [{"nameIdentifier": "163425", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "\u9ad8\u91ce, \u88d5\u5b50"}]}, {"nameIdentifiers": [{"nameIdentifier": "163426", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "\u65b0\u6751, \u672b\u96c4"}]}]}, "item_7_publisher_7": {"attribute_name": "\u51fa\u7248\u8005", "attribute_value_mlt": [{"subitem_publisher": "\u65b0\u6f5f\u5927\u5b66\u8fb2\u5b66\u90e8"}]}, "item_7_select_19": {"attribute_name": "\u8457\u8005\u7248\u30d5\u30e9\u30b0", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_7_source_id_11": {"attribute_name": "\u66f8\u8a8c\u30ec\u30b3\u30fc\u30c9ID", "attribute_value_mlt": [{"subitem_source_identifier": "AN00183393", "subitem_source_identifier_type": "NCID"}]}, "item_7_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "03858634", "subitem_source_identifier_type": "ISSN"}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Takano, Hiroko"}], "nameIdentifiers": [{"nameIdentifier": "163423", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Niimura, Sueo"}], "nameIdentifiers": [{"nameIdentifier": "163424", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-08-20"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "123-128.pdf", "filesize": [{"value": "776.6 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 776600.0, "url": {"label": "123-128.pdf", "url": "https://niigata-u.repo.nii.ac.jp/record/28407/files/123-128.pdf"}, "version_id": "90df3518-90a6-4379-8afe-ccd265f2ed1e"}]}, "item_keyword": {"attribute_name": "\u30ad\u30fc\u30ef\u30fc\u30c9", "attribute_value_mlt": [{"subitem_subject": "porcine oocyte", "subitem_subject_scheme": "Other"}, {"subitem_subject": "cAMP", "subitem_subject_scheme": "Other"}, {"subitem_subject": "distribution of cortical granule", "subitem_subject_scheme": "Other"}, {"subitem_subject": "lectin-histochemistry", "subitem_subject_scheme": "Other"}, {"subitem_subject": "nuclear maturation", "subitem_subject_scheme": "Other"}, {"subitem_subject": "\u30d6\u30bf\u5375\u6bcd\u7d30\u80de", "subitem_subject_scheme": "Other"}, {"subitem_subject": "\u8868\u5c64\u7c92\u306e\u5206\u5e03", "subitem_subject_scheme": "Other"}, {"subitem_subject": "\u30ec\u30af\u30c1\u30f3\u7d44\u7e54\u5316\u5b66", "subitem_subject_scheme": "Other"}, {"subitem_subject": "\u6838\u306e\u6210\u719f", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Nuclear Maturation and Cortical Granule Distribution in Porcine Oocytes Treated with IBMX and dbcAMP", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "Nuclear Maturation and Cortical Granule Distribution in Porcine Oocytes Treated with IBMX and dbcAMP"}, {"subitem_title": "Nuclear Maturation and Cortical Granule Distribution in Porcine Oocytes Treated with IBMX and dbcAMP", "subitem_title_language": "en"}]}, "item_type_id": "7", "owner": "1", "path": ["453/456", "485/872/1583/1601"], "permalink_uri": "http://hdl.handle.net/10191/6422", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_value": "2008-05-02"}, "publish_date": "2008-05-02", "publish_status": "0", "recid": "28407", "relation": {}, "relation_version_is_last": true, "title": ["Nuclear Maturation and Cortical Granule Distribution in Porcine Oocytes Treated with IBMX and dbcAMP"], "weko_shared_id": null}