{"_buckets": {"deposit": "ea45c5de-a077-451c-91f2-09847af6beaf"}, "_deposit": {"id": "28377", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "28377"}, "status": "published"}, "_oai": {"id": "oai:niigata-u.repo.nii.ac.jp:00028377", "sets": ["456", "1598"]}, "item_7_alternative_title_1": {"attribute_name": "その他のタイトル", "attribute_value_mlt": [{"subitem_alternative_title": "イネ葉緑体のグライコプロテオーム解析"}]}, "item_7_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2009-09", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "1", "bibliographicPageEnd": "30", "bibliographicPageStart": "25", "bibliographicVolumeNumber": "62", "bibliographic_titles": [{"bibliographic_title": "新潟大学農学部研究報告"}, {"bibliographic_title": "新潟大学農学部研究報告", "bibliographic_titleLang": "en"}]}]}, "item_7_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "We performed a glycoproteomic analysis of rice chloroplast. Intact chloroplasts were isolated from mature leaves of rice using discontinuous Percoll density gradient centrifugation. The isolated chloroplast proteins were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis, followed by lectinoblotting with peroxidase-conjugated Concanavalin A. Glycoproteins detected with Concanavalin A-peroxidase were analyzed by a mass spectrometry, indicating that phosphoglycerate kinase-like protein which has signal peptide and N-linked oligosaccharide chains occurs in chloroplasts of rice.", "subitem_description_type": "Abstract"}, {"subitem_description": "イネ葉緑体タンパク質のグライコプロテオーム解析を行った。イネ成熟葉から不連続パーコール密度勾配遠心分離法を用いて無傷の葉緑体を単離した。葉緑体タンパク質を調製し、SDS-ポリアクリルアミドゲル電気泳動の後、ペルオキシダーゼ標識Concanavalin Aを用いたレクチンブロッティングを行った。ペルオキシダーゼ標識Concanavalin Aにより検出されたいくつかのタンパクバンドを質量分析装置を用いて解析した結果、ERシグナルペプチドとN結合型糖鎖結合部位を持つホスホグリセリン酸キナーゼ様タンパク質がイネ葉緑体に局在していることが示唆された。", "subitem_description_type": "Abstract"}]}, "item_7_full_name_3": {"attribute_name": "著者別名", "attribute_value_mlt": [{"nameIdentifiers": [{"nameIdentifier": "163248", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "濵田, 侑紀"}]}, {"nameIdentifiers": [{"nameIdentifier": "163249", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "北嶋, 彩"}]}, {"nameIdentifiers": [{"nameIdentifier": "163250", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "岡田, 久夫"}]}, {"nameIdentifiers": [{"nameIdentifier": "163251", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "大久保, 英奈"}]}, {"nameIdentifiers": [{"nameIdentifier": "163252", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "金古, 堅太郎"}]}, {"nameIdentifiers": [{"nameIdentifier": "163253", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "高山, 和俊"}]}, {"nameIdentifiers": [{"nameIdentifier": "163254", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "堀, 秀隆"}]}, {"nameIdentifiers": [{"nameIdentifier": "163255", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "伊藤, 紀美子"}]}, {"nameIdentifiers": [{"nameIdentifier": "163256", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "三ツ井, 敏明"}]}]}, "item_7_publisher_7": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "新潟大学農学部"}]}, "item_7_select_19": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_7_source_id_11": {"attribute_name": "書誌レコードID", "attribute_value_mlt": [{"subitem_source_identifier": "AN00183393", "subitem_source_identifier_type": "NCID"}]}, "item_7_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "03858634", "subitem_source_identifier_type": "ISSN"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Hamada, Yuki"}], "nameIdentifiers": [{"nameIdentifier": "163238", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kitajima, Aya"}], "nameIdentifiers": [{"nameIdentifier": "163239", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Okada, Hisao"}], "nameIdentifiers": [{"nameIdentifier": "163240", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Okubo, Ena"}], "nameIdentifiers": [{"nameIdentifier": "163241", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Awang, Azwan"}], "nameIdentifiers": [{"nameIdentifier": "163242", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kaneko, Kentaro"}], "nameIdentifiers": [{"nameIdentifier": "163243", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Takayama, Kazutoshi"}], "nameIdentifiers": [{"nameIdentifier": "163244", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Hori, Hidetaka"}], "nameIdentifiers": [{"nameIdentifier": "163245", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Itoh, Kimiko"}], "nameIdentifiers": [{"nameIdentifier": "163246", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Mitsui, Toshiaki"}], "nameIdentifiers": [{"nameIdentifier": "163247", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-08-20"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "62(1)_25-30.pdf", "filesize": [{"value": "1.0 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 1000000.0, "url": {"label": "62(1)_25-30.pdf", "url": "https://niigata-u.repo.nii.ac.jp/record/28377/files/62(1)_25-30.pdf"}, "version_id": "282cd8a9-0b14-4aa6-807e-1e4b23e54d7d"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "chloroplast", "subitem_subject_scheme": "Other"}, {"subitem_subject": "glycoproteomics", "subitem_subject_scheme": "Other"}, {"subitem_subject": "lectin staining", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Oryza sativa L.", "subitem_subject_scheme": "Other"}, {"subitem_subject": "phosphoglycerate kinase", "subitem_subject_scheme": "Other"}, {"subitem_subject": "イネ", "subitem_subject_scheme": "Other"}, {"subitem_subject": "グライコプロテオミクス", "subitem_subject_scheme": "Other"}, {"subitem_subject": "ホスホグリセリン酸キナーゼ", "subitem_subject_scheme": "Other"}, {"subitem_subject": "レクチン染色", "subitem_subject_scheme": "Other"}, {"subitem_subject": "葉緑体", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "jpn"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Glycoproteomic Analysis of Chloroplasts Isolated from Rice Mature Leaves", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Glycoproteomic Analysis of Chloroplasts Isolated from Rice Mature Leaves"}, {"subitem_title": "Glycoproteomic Analysis of Chloroplasts Isolated from Rice Mature Leaves", "subitem_title_language": "en"}]}, "item_type_id": "7", "owner": "1", "path": ["456", "1598"], "permalink_uri": "http://hdl.handle.net/10191/13097", "pubdate": {"attribute_name": "公開日", "attribute_value": "2010-07-14"}, "publish_date": "2010-07-14", "publish_status": "0", "recid": "28377", "relation": {}, "relation_version_is_last": true, "title": ["Glycoproteomic Analysis of Chloroplasts Isolated from Rice Mature Leaves"], "weko_shared_id": null}