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Glycoproteomic Analysis of Chloroplasts Isolated from Rice Mature Leaves
http://hdl.handle.net/10191/13097
1ef03e49-0752-48ff-b36e-f207e0ac98db
名前 / ファイル | ライセンス | アクション | |
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2010-07-14 | |||||
タイトル | ||||||
タイトル | Glycoproteomic Analysis of Chloroplasts Isolated from Rice Mature Leaves | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Glycoproteomic Analysis of Chloroplasts Isolated from Rice Mature Leaves | |||||
言語 | ||||||
言語 | jpn | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | chloroplast | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | glycoproteomics | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | lectin staining | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Oryza sativa L. | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | phosphoglycerate kinase | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | イネ | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | グライコプロテオミクス | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | ホスホグリセリン酸キナーゼ | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | レクチン染色 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 葉緑体 | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | departmental bulletin paper | |||||
その他のタイトル | ||||||
その他のタイトル | イネ葉緑体のグライコプロテオーム解析 | |||||
著者 |
Hamada, Yuki
× Hamada, Yuki× Kitajima, Aya× Okada, Hisao× Okubo, Ena× Awang, Azwan× Kaneko, Kentaro× Takayama, Kazutoshi× Hori, Hidetaka× Itoh, Kimiko× Mitsui, Toshiaki |
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著者別名 | ||||||
識別子 | ||||||
識別子 | 163248 | |||||
識別子Scheme | WEKO | |||||
姓名 | ||||||
姓名 | 濵田, 侑紀 | |||||
著者別名 | ||||||
識別子 | ||||||
識別子 | 163249 | |||||
識別子Scheme | WEKO | |||||
姓名 | ||||||
姓名 | 北嶋, 彩 | |||||
著者別名 | ||||||
識別子 | ||||||
識別子 | 163250 | |||||
識別子Scheme | WEKO | |||||
姓名 | ||||||
姓名 | 岡田, 久夫 | |||||
著者別名 | ||||||
識別子 | ||||||
識別子 | 163251 | |||||
識別子Scheme | WEKO | |||||
姓名 | ||||||
姓名 | 大久保, 英奈 | |||||
著者別名 | ||||||
識別子 | ||||||
識別子 | 163252 | |||||
識別子Scheme | WEKO | |||||
姓名 | ||||||
姓名 | 金古, 堅太郎 | |||||
著者別名 | ||||||
識別子 | ||||||
識別子 | 163253 | |||||
識別子Scheme | WEKO | |||||
姓名 | ||||||
姓名 | 高山, 和俊 | |||||
著者別名 | ||||||
識別子 | ||||||
識別子 | 163254 | |||||
識別子Scheme | WEKO | |||||
姓名 | ||||||
姓名 | 堀, 秀隆 | |||||
著者別名 | ||||||
識別子 | ||||||
識別子 | 163255 | |||||
識別子Scheme | WEKO | |||||
姓名 | ||||||
姓名 | 伊藤, 紀美子 | |||||
著者別名 | ||||||
識別子 | ||||||
識別子 | 163256 | |||||
識別子Scheme | WEKO | |||||
姓名 | ||||||
姓名 | 三ツ井, 敏明 | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | We performed a glycoproteomic analysis of rice chloroplast. Intact chloroplasts were isolated from mature leaves of rice using discontinuous Percoll density gradient centrifugation. The isolated chloroplast proteins were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis, followed by lectinoblotting with peroxidase-conjugated Concanavalin A. Glycoproteins detected with Concanavalin A-peroxidase were analyzed by a mass spectrometry, indicating that phosphoglycerate kinase-like protein which has signal peptide and N-linked oligosaccharide chains occurs in chloroplasts of rice. | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | イネ葉緑体タンパク質のグライコプロテオーム解析を行った。イネ成熟葉から不連続パーコール密度勾配遠心分離法を用いて無傷の葉緑体を単離した。葉緑体タンパク質を調製し、SDS-ポリアクリルアミドゲル電気泳動の後、ペルオキシダーゼ標識Concanavalin Aを用いたレクチンブロッティングを行った。ペルオキシダーゼ標識Concanavalin Aにより検出されたいくつかのタンパクバンドを質量分析装置を用いて解析した結果、ERシグナルペプチドとN結合型糖鎖結合部位を持つホスホグリセリン酸キナーゼ様タンパク質がイネ葉緑体に局在していることが示唆された。 | |||||
書誌情報 |
新潟大学農学部研究報告 en : 新潟大学農学部研究報告 巻 62, 号 1, p. 25-30, 発行日 2009-09 |
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出版者 | ||||||
出版者 | 新潟大学農学部 | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 03858634 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AN00183393 | |||||
著者版フラグ | ||||||
値 | publisher |