{"_buckets": {"deposit": "97ecd6a7-6854-4a0b-8276-89c38183f940"}, "_deposit": {"id": "28325", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "28325"}, "status": "published"}, "_oai": {"id": "oai:niigata-u.repo.nii.ac.jp:00028325"}, "item_7_alternative_title_1": {"attribute_name": "\u305d\u306e\u4ed6\u306e\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_alternative_title": "\u65b0\u898f\u30cb\u30b2\u30ed\u30fc\u30b9\u30db\u30b9\u30db\u30ea\u30e9\u30fc\u30bc\u3092\u7528\u3044\u305f\u03b1-1,3-\u30b0\u30eb\u30b32\u7cd6\u306e\u751f\u7523"}]}, "item_7_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2012-03", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "2", "bibliographicPageEnd": "113", "bibliographicPageStart": "107", "bibliographicVolumeNumber": "64", "bibliographic_titles": [{"bibliographic_title": "\u65b0\u6f5f\u5927\u5b66\u8fb2\u5b66\u90e8\u7814\u7a76\u5831\u544a"}, {"bibliographic_title": "\u65b0\u6f5f\u5927\u5b66\u8fb2\u5b66\u90e8\u7814\u7a76\u5831\u544a", "bibliographic_titleLang": "en"}]}]}, "item_7_description_4": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "A novel phosphorylase (Cphy1874) belonging to glycoside hydrolase family 65 was first characterized from Clostridiumphytofermentans. Recombinant Cphy1874 protein produced in Escherichia coli showed phosphorolytic activity towardnigerose in the presence of inorganic phosphate, resulting in release of D-glucose and \u03b2-D-glucose 1-phosphate with inversionof the anomeric configuration. Kinetic parameters of the phosphorolytic activity on nigerose were kcat 67 s-1 and Km 1.7 mM.Additionally Cphy1874 showed the highest synthetic activity with D-glucose as the acceptor in the reverse reaction with \u03b2-D-glucose 1-phosphate as the donor. The synthesized product was mostly nigerose at the early-stage of the reaction.\u3000Theenzyme also showed synthetic activity with D-xylose, 1,5-anhydro-D-glucitol, D-galactose, and methyl-\u03b1-D-glucoside in thisorder. All the major products were \u03b1-1,3-glucosyl disaccharides. We proposed nigerose phosphorylase as the short name ofthe enzyme.", "subitem_description_type": "Abstract"}, {"subitem_description": "\u7cd6\u8cea\u52a0\u30ea\u30f3\u9178\u5206\u89e3\u9175\u7d20\uff08\u30db\u30b9\u30db\u30ea\u30e9\u30fc\u30bc\uff09\u306f\u3001\u305d\u306e\u53b3\u5bc6\u306a\u57fa\u8cea\u7279\u7570\u6027\u3068\u53cd\u5fdc\u306e\u53ef\u9006\u6027\u304b\u3089\u30aa\u30ea\u30b4\u7cd6\u5408\u6210\u306b\u5229\u7528\u53ef\u80fd\u3067\u3042\u308b\u3002\u3057\u304b\u3057\u65e2\u77e5\u306e\u30db\u30b9\u30db\u30ea\u30e9\u30fc\u30bc\u306f15\u7a2e\u985e\u306e\u307f\u3067\u3001\u65b0\u305f\u306a\u57fa\u8cea\u7279\u7570\u6027\u3092\u6709\u3059\u308b\u65b0\u898f\u30db\u30b9\u30db\u30ea\u30e9\u30fc\u30bc\u306e\u767a\u898b\u304c\u5f37\u304f\u671b\u307e\u308c\u3066\u3044\u308b\u3002\u305d\u3053\u3067\u3001\u3053\u308c\u307e\u3067\u6570\u7a2e\u306e\u30db\u30b9\u30db\u30ea\u30e9\u30fc\u30bc\u304c\u5358\u96e2\u3055\u308c\u3066\u3044\u308bClostridium phytofermentans \u304c\u6709\u3059\u308bGlycoside Hydrolase Family 65\u306b\u5c5e\u3059\u308b\u907a\u4f1d\u5b50\uff08cphy1874\uff09\u3092\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u3057\u3001\u7d44\u63db\u3048\u9175\u7d20\u306e\u9175\u7d20\u5316\u5b66\u7684\u6027\u8cea\u3092\u8abf\u67fb\u3057\u305f\u3002\u7d50\u679c\u3001Cphy1874\u306f\u30ea\u30f3\u9178\u5b58\u5728\u4e0b\u3067\u30cb\u30b2\u30ed\u30fc\u30b9\u306b\u5bfe\u3057\u9ad8\u3044\u52a0\u30ea\u30f3\u9178\u5206\u89e3\u6d3b\u6027\u3092\u793a\u3059\u3053\u3068\u304c\u660e\u3089\u304b\u306b\u306a\u3063\u305f\u3002\u03b2- \u30b0\u30eb\u30b3\u30fc\u30b91\u30ea\u30f3\u9178\u3068\u5404\u7a2e\u7cd6\u3092\u4f5c\u7528\u3055\u305b\u305f\u3068\u3053\u308d\uff0c\u30b0\u30eb\u30b3\u30fc\u30b9\u3092\u30a2\u30af\u30bb\u30d7\u30bf\u30fc\u3068\u3057\u305f\u969b\u306b\u9ad8\u3044\u5408\u6210\u6d3b\u6027\u3092\u793a\u3057\u305f\u3002\u30ad\u30b7\u30ed\u30fc\u30b9\u30011,5- \u30a2\u30f3\u30d2\u30c9\u30ed\u30b0\u30eb\u30b7\u30c8\u30fc\u30eb\u3001\u30ac\u30e9\u30af\u30c8\u30fc\u30b9\u3001\u30e1\u30c1\u30eb- \u03b1 - \u30b0\u30eb\u30b3\u30b7\u30c9\u306b\u304a\u3044\u3066\u3082\u30a2\u30af\u30bb\u30d7\u30bf\u30fc\u3068\u306a\u308a\u3001\u4e3b\u751f\u6210\u7269\u306f\u3044\u305a\u308c\u3082\u03b1-1,3\u7d50\u5408\u3067\u3042\u3063\u305f\u3002\u3053\u306e\u3053\u3068\u304b\u3089\u672c\u9175\u7d20\u306f\uff0c\u3053\u308c\u307e\u3067\u5831\u544a\u306e\u7121\u304b\u3063\u305f\u65b0\u898f\u52a0\u30ea\u30f3\u9178\u5206\u89e3\u9175\u7d20\u30cb\u30b2\u30ed\u30fc\u30b9\u30db\u30b9\u30db\u30ea\u30e9\u30fc\u30bc\u3067\u3042\u308b\u3053\u3068\u304c\u5224\u660e\u3057\u305f\u3002", "subitem_description_type": "Abstract"}]}, "item_7_full_name_3": {"attribute_name": "\u8457\u8005\u5225\u540d", "attribute_value_mlt": [{"nameIdentifiers": [{"nameIdentifier": "162830", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "\u4ec1\u5e73, \u9ad8\u5247"}]}, {"nameIdentifiers": [{"nameIdentifier": "162831", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "\u4e2d\u4e95, \u535a\u4e4b"}]}]}, "item_7_publisher_7": {"attribute_name": "\u51fa\u7248\u8005", "attribute_value_mlt": [{"subitem_publisher": "\u65b0\u6f5f\u5927\u5b66\u8fb2\u5b66\u90e8"}]}, "item_7_select_19": {"attribute_name": "\u8457\u8005\u7248\u30d5\u30e9\u30b0", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_7_source_id_11": {"attribute_name": "\u66f8\u8a8c\u30ec\u30b3\u30fc\u30c9ID", "attribute_value_mlt": [{"subitem_source_identifier": "AN00183393", "subitem_source_identifier_type": "NCID"}]}, "item_7_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "03858634", "subitem_source_identifier_type": "ISSN"}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Nihira, Takanori"}], "nameIdentifiers": [{"nameIdentifier": "162827", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nakai, Hiroyuki"}], "nameIdentifiers": [{"nameIdentifier": "162828", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kitaoka, Motomitsu"}], "nameIdentifiers": [{"nameIdentifier": "162829", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-08-20"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "64(2)_107-113.pdf", "filesize": [{"value": "753.2 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 753200.0, "url": {"label": "64(2)_107-113.pdf", "url": "https://niigata-u.repo.nii.ac.jp/record/28325/files/64(2)_107-113.pdf"}, "version_id": "09615f46-8daf-481f-b425-b94631efd999"}]}, "item_keyword": {"attribute_name": "\u30ad\u30fc\u30ef\u30fc\u30c9", "attribute_value_mlt": [{"subitem_subject": "nigerose phosphorylase", "subitem_subject_scheme": "Other"}, {"subitem_subject": "glycoside hydrolase family 65", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Clostridium phytofermentans", "subitem_subject_scheme": "Other"}, {"subitem_subject": "\u30cb\u30b2\u30ed\u30fc\u30b9\u30db\u30b9\u30db\u30ea\u30e9\u30fc\u30bc", "subitem_subject_scheme": "Other"}, {"subitem_subject": "\u30b0\u30eb\u30b3\u30b7\u30c9\u30cf\u30a4\u30c9\u30ed\u30e9\u30fc\u30bc\u30d5\u30a1\u30df\u30ea\u30fc32", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Enzymatic Synthesis of \u03b1-1,3-Glucosyl Disaccharides by Novel Nigerose Phosphorylase from Clostridium phytofermentans", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "Enzymatic Synthesis of \u03b1-1,3-Glucosyl Disaccharides by Novel Nigerose Phosphorylase from Clostridium phytofermentans"}, {"subitem_title": "Enzymatic Synthesis of \u03b1-1,3-Glucosyl Disaccharides by Novel Nigerose Phosphorylase from Clostridium phytofermentans", "subitem_title_language": "en"}]}, "item_type_id": "7", "owner": "1", "path": ["453/456", "485/872/1583/1593"], "permalink_uri": "http://hdl.handle.net/10191/18638", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_value": "2012-07-02"}, "publish_date": "2012-07-02", "publish_status": "0", "recid": "28325", "relation": {}, "relation_version_is_last": true, "title": ["Enzymatic Synthesis of \u03b1-1,3-Glucosyl Disaccharides by Novel Nigerose Phosphorylase from Clostridium phytofermentans"], "weko_shared_id": null}