{"_buckets": {"deposit": "3c4a98db-8d60-4145-b123-5ee59393a2ed"}, "_deposit": {"id": "28324", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "28324"}, "status": "published"}, "_oai": {"id": "oai:niigata-u.repo.nii.ac.jp:00028324", "sets": ["456", "1593"]}, "item_7_alternative_title_1": {"attribute_name": "その他のタイトル", "attribute_value_mlt": [{"subitem_alternative_title": "培養物へのオリザリン処理による日本原産センノウ属植物の染色体倍加"}]}, "item_7_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2012-03", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "2", "bibliographicPageEnd": "105", "bibliographicPageStart": "101", "bibliographicVolumeNumber": "64", "bibliographic_titles": [{"bibliographic_title": "新潟大学農学部研究報告"}, {"bibliographic_title": "新潟大学農学部研究報告", "bibliographic_titleLang": "en"}]}]}, "item_7_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "The genus Lychnis (Caryophyllaceae) consists of about 30 species, among which 6 are native to Japan. Many Lychnisspp. are cultivated as pot or garden plants for their high ornamental value. In the present study, chromosome doublingof 3 Japanese native Lychnis spp., L. gracillima (2n=2x=24), L. kiusiana (2n=2x=24) and L. miqueliana (2n=2x=24), wasexamined for widening their variability in horticultural traits by using an in vitro oryzalin (ORY) treatment method, whichwas initially developed for a triploid genotype of L. senno (Nonaka et al., 2011). Nodal segments prepared from in vitrogrownplantlets of these 3 species were treated with 10 mg L-1 ORY for 24 h. Two months after ORY treatment, more than70% of nodal segments survived in all the 3 Lychnis spp. Flow cytometry analysis of leaf tissues showed that 15.7, 15.1 and7.8% of plantlets, which were derived from ORY-treated nodal segments, were tetraploid (4x) or ploidy chimera (2x+4x)in L. gracillima, L. kiusiana and L. miqueliana, respectively. Chromosome doubling was also confirmed by chromosomeobservation in root-tip cells. These results indicate that in vitro ORY treatment may be applicable for chromosome doublingof a wide range of Lychnis spp. Tetraploid plantlets obtained in the present study showed a compact plant form comparedwith diploid plantlets.", "subitem_description_type": "Abstract"}, {"subitem_description": "センノウ属（Lychnis spp.；ナデシコ科）は約30種で構成され、そのうちの６種は日本に自生している。多くのセンノウ属植物は観賞的価値が高く、そのため鉢植え植物や庭植え植物として栽培されている。本研究では、園芸形質の拡大を目的として、日本原産の３種のセンノウ属植物、センジュガンピ（L. gracillima；2n=2x=24）、オグラセンノウ（L. kiusiana；2n=2x=24）およびフシグロセンノウ（L. miqueliana；2n=2x=24）について、培養物のオリザリン（ORY）処理による染色体倍加を試みた。これは、三倍体のセンノウ（L. senno）において最初に確立された方法である（Nonaka ら、2011）。培養小植物体から調製した節切片を10 mg L-1 ORY で24時間処理したところ、２ヵ月後にはいずれのセンノウ属植物においても70％ 以上の節切片が生存していた。また、ORY 処理した節切片由来の小植物体についてフローサイトメトリー分析を行ったところ、センジュガンピ、オグラセンノウおよびフシグロセンノウの小植物体のそれぞれ15.7、15.1および7.8％ が四倍体（4x）または倍数性キメラ（2x+4x）であった。染色体倍加は、根端細胞の染色体観察によっても確認された。以上の結果は、培養物へのORY 処理が、広範囲のセンノウ属植物における染色体倍加に適用できることを示している。本研究で得られた四倍体小植物体は、二倍体小植物体と比較して、草姿が小型であった。", "subitem_description_type": "Abstract"}]}, "item_7_full_name_3": {"attribute_name": "著者別名", "attribute_value_mlt": [{"nameIdentifiers": [{"nameIdentifier": "162825", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "中野, 優"}]}, {"nameIdentifiers": [{"nameIdentifier": "162826", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "田崎, 大夢"}]}]}, "item_7_publisher_7": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "新潟大学農学部"}]}, "item_7_select_19": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_7_source_id_11": {"attribute_name": "書誌レコードID", "attribute_value_mlt": [{"subitem_source_identifier": "AN00183393", "subitem_source_identifier_type": "NCID"}]}, "item_7_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "03858634", "subitem_source_identifier_type": "ISSN"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Nakano, Masaru"}], "nameIdentifiers": [{"nameIdentifier": "162820", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Tasaki, Hiromu"}], "nameIdentifiers": [{"nameIdentifier": "162821", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nonaka, Takahiro"}], "nameIdentifiers": [{"nameIdentifier": "162822", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Han, Dong-Sheng"}], "nameIdentifiers": [{"nameIdentifier": "162823", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Godo, Toshinari"}], "nameIdentifiers": [{"nameIdentifier": "162824", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-08-20"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "64(2)_101-105.pdf", "filesize": [{"value": "682.3 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 682300.0, "url": {"label": "64(2)_101-105.pdf", "url": "https://niigata-u.repo.nii.ac.jp/record/28324/files/64(2)_101-105.pdf"}, "version_id": "c75528fe-54d2-4788-af7e-462e4ee39126"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "caryophyllaceous ornamental", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Lychnis gracillima", "subitem_subject_scheme": "Other"}, {"subitem_subject": "L kiusiana", "subitem_subject_scheme": "Other"}, {"subitem_subject": "L miqueliana", "subitem_subject_scheme": "Other"}, {"subitem_subject": "tetraploid", "subitem_subject_scheme": "Other"}, {"subitem_subject": "オグラセンノウ", "subitem_subject_scheme": "Other"}, {"subitem_subject": "センジュガンピ", "subitem_subject_scheme": "Other"}, {"subitem_subject": "ナデシコ科観賞植物", "subitem_subject_scheme": "Other"}, {"subitem_subject": "フシグロセンノウ", "subitem_subject_scheme": "Other"}, {"subitem_subject": "四倍体", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Chromosome Doubling of Japanese Native Lychnis spp. by in vitro Oryzalin Treatment", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Chromosome Doubling of Japanese Native Lychnis spp. by in vitro Oryzalin Treatment"}, {"subitem_title": "Chromosome Doubling of Japanese Native Lychnis spp. by in vitro Oryzalin Treatment", "subitem_title_language": "en"}]}, "item_type_id": "7", "owner": "1", "path": ["456", "1593"], "permalink_uri": "http://hdl.handle.net/10191/18634", "pubdate": {"attribute_name": "公開日", "attribute_value": "2012-07-02"}, "publish_date": "2012-07-02", "publish_status": "0", "recid": "28324", "relation": {}, "relation_version_is_last": true, "title": ["Chromosome Doubling of Japanese Native Lychnis spp. by in vitro Oryzalin Treatment"], "weko_shared_id": null}