{"_buckets": {"deposit": "dd4505e8-80a0-498a-8744-75840f17fc0a"}, "_deposit": {"id": "28319", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "28319"}, "status": "published"}, "_oai": {"id": "oai:niigata-u.repo.nii.ac.jp:00028319"}, "item_7_alternative_title_1": {"attribute_name": "\u305d\u306e\u4ed6\u306e\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_alternative_title": "\u30de\u30eb\u30c8\u30fc\u30b9\u30db\u30b9\u30db\u30ea\u30e9\u30fc\u30bc\u3092\u7528\u3044\u305f\u5206\u5c90\u30b0\u30eb\u30b3\uff13\u7cd6\u306e\u751f\u7523"}]}, "item_7_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2012-09", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "1", "bibliographicPageEnd": "75", "bibliographicPageStart": "67", "bibliographicVolumeNumber": "65", "bibliographic_titles": [{"bibliographic_title": "\u65b0\u6f5f\u5927\u5b66\u8fb2\u5b66\u90e8\u7814\u7a76\u5831\u544a"}, {"bibliographic_title": "\u65b0\u6f5f\u5927\u5b66\u8fb2\u5b66\u90e8\u7814\u7a76\u5831\u544a", "bibliographic_titleLang": "en"}]}]}, "item_7_description_4": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "We discovered an inverting maltose phosphorylase (Bsel2056) belonging to glycoside hydrolase family 65 from Bacillus selenitireducens MLS10, which possesses synthetic ability for \u03b1-D-glucosyl disaccharides and trisaccharides through the reverse phosphorolysis with \u03b2-D-glucose 1-phosphate as the donor. Bsel2056 showed the preference for monosaccharide acceptors with alternative C2 substituent (2-amino-2-deoxy-D-glucose, 2-deoxy-D-arabino-hexose, 2-acetamido-2-deoxy-D-glucose, D-mannose), resulting in production of 1,4-\u03b1-D-glucosyl disaccharides with strict regioselectivity. In addition, Bsel2056 synthesized two maltose derivatives possessing additional D-glucosyl residue bound to C2 position of the D-glucose residue at the reducing end, 1,4-\u03b1-D-glucopyranosyl-[1,2-\u03b1-D-glucopyranosyl]-D-glucose and 1,4-\u03b1-D-glucopyranosyl-[1,2-\u03b2-D-glucopyranosyl]-D-glucose, from 1,2-\u03b1-D-glucopyranosyl-D-glucose (kojibiose) and 1,2-\u03b2-D-glucopyranosyl-D-glucose (sophorose), respectively, as the acceptors. Modeling structure analysis using Lactobacillus brevis GH65 maltose phosphorylase as the template and superimposition of an inhibitory pseudomaltotetrasacchaide acarbose from a complex with Aspergillus awamori GH15 glucoamylase suggested that Bsel2056 possessed a binding space to accommodate the bulky C2 substituent of D-glucose.", "subitem_description_type": "Abstract"}, {"subitem_description": 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{"subitem_title": "Enzymatic synthesis of branched \u03b1-D-glucosyl trisaccharides by novel maltosephosphorylase from Bacillus selenitireducens MLS10", "subitem_title_language": "en"}]}, "item_type_id": "7", "owner": "1", "path": ["453/456", "485/872/1583/1592"], "permalink_uri": "http://hdl.handle.net/10191/22583", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_value": "2013-05-29"}, "publish_date": "2013-05-29", "publish_status": "0", "recid": "28319", "relation": {}, "relation_version_is_last": true, "title": ["Enzymatic synthesis of branched \u03b1-D-glucosyl trisaccharides by novel maltosephosphorylase from Bacillus selenitireducens MLS10"], "weko_shared_id": null}