{"_buckets": {"deposit": "7f2ae1e3-6d11-4507-bef6-8aed2462ff79"}, "_deposit": {"id": "28315", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "28315"}, "status": "published"}, "_oai": {"id": "oai:niigata-u.repo.nii.ac.jp:00028315", "sets": ["456", "1592"]}, "item_7_alternative_title_1": {"attribute_name": "その他のタイトル", "attribute_value_mlt": [{"subitem_alternative_title": "シロイヌナズナにおける葯特異的遺伝子のプロモーター解析"}]}, "item_7_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2012-09", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "1", "bibliographicPageEnd": "37", "bibliographicPageStart": "31", "bibliographicVolumeNumber": "65", "bibliographic_titles": [{"bibliographic_title": "新潟大学農学部研究報告"}, {"bibliographic_title": "新潟大学農学部研究報告", "bibliographic_titleLang": "en"}]}]}, "item_7_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Anther-specific genes and their promoters have been extensively studied to identify genes necessary for anther development or genetic engineering of male-sterile plants. In this study, we obtained promoter sequences of six genes in Arabidopsis thaliana, and these promoters fused to the β-glucuronidase (GUS) gene were transformed into A. thaliana to monitor their expression patterns throughout anther development. The GUS expression patterns confirmed the expression in several stages of anther development. Sequence analysis revealed that the promoters contain cis-acting elements known to confer anther/pollen-specific gene expression, supporting their anther specific expression pattern.", "subitem_description_type": "Abstract"}, {"subitem_description": "葯特異的遺伝子やそれらのプロモーター配列の解析は、葯発達に関わる遺伝子の同定や、遺伝子組換えによる雄性不稔植物の作出を目的として、これまで多くなされてきている。本研究では、６つのプロモーター配列の単離を行い、GUS 遺伝子に連結してシロイヌナズナに導入することで、葯の発達段階における発現誘導様式を調べた。プロモーターGUS の発現は、様々な花粉発達ステージにおいて観察された。また、塩基配列の解析では葯/ 花粉特異的遺伝子の発現に必要とされるシス配列が多く含まれていたことから、これらのシス配列が葯特異的に発現誘導していることが示唆された。", "subitem_description_type": "Abstract"}]}, "item_7_full_name_3": {"attribute_name": "著者別名", "attribute_value_mlt": [{"nameIdentifiers": [{"nameIdentifier": "162755", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "川辺, 隆大"}]}, {"nameIdentifiers": [{"nameIdentifier": "162756", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "長部, 謙二"}]}, {"nameIdentifiers": [{"nameIdentifier": "162757", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "藤本, 龍"}]}]}, "item_7_publisher_7": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "新潟大学農学部"}]}, "item_7_select_19": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_7_source_id_11": {"attribute_name": "書誌レコードID", "attribute_value_mlt": [{"subitem_source_identifier": "AN00183393", "subitem_source_identifier_type": "NCID"}]}, "item_7_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "03858634", "subitem_source_identifier_type": "ISSN"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Kawanabe, Takahiro"}], "nameIdentifiers": [{"nameIdentifier": "162752", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Osabe, Kenji"}], "nameIdentifiers": [{"nameIdentifier": "162753", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Fujimoto, ryo"}], "nameIdentifiers": [{"nameIdentifier": "162754", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-08-20"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "65(1)_31-37.pdf", "filesize": [{"value": "795.1 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 795100.0, "url": {"label": "65(1)_31-37.pdf", "url": "https://niigata-u.repo.nii.ac.jp/record/28315/files/65(1)_31-37.pdf"}, "version_id": "0d54fefe-ed75-41d3-812b-5fcc9ecb8f57"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "Arabidopsis thaliana", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Anther-specific promoter", "subitem_subject_scheme": "Other"}, {"subitem_subject": "GUS staining", "subitem_subject_scheme": "Other"}, {"subitem_subject": "cis-acting elements", "subitem_subject_scheme": "Other"}, {"subitem_subject": "葯特異的プロモーター", "subitem_subject_scheme": "Other"}, {"subitem_subject": "GUS染色", "subitem_subject_scheme": "Other"}, {"subitem_subject": "シスエレメント", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Analysis of the promoter activity of anther-specific genes in Arabidopsis thaliana", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Analysis of the promoter activity of anther-specific genes in Arabidopsis thaliana"}, {"subitem_title": "Analysis of the promoter activity of anther-specific genes in Arabidopsis thaliana", "subitem_title_language": "en"}]}, "item_type_id": "7", "owner": "1", "path": ["456", "1592"], "permalink_uri": "http://hdl.handle.net/10191/22570", "pubdate": {"attribute_name": "公開日", "attribute_value": "2013-05-29"}, "publish_date": "2013-05-29", "publish_status": "0", "recid": "28315", "relation": {}, "relation_version_is_last": true, "title": ["Analysis of the promoter activity of anther-specific genes in Arabidopsis thaliana"], "weko_shared_id": null}