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In addition, the size of the perivitelline space of DOs cultured in modified NCSU37 with decreased concentration of NaCl and those cultured in NCSU37 containing 4-methylumbelliferone (MU), an inhibitor of hyaluronic acid synthase, was observed. The incidence of polyspermy in DOs matured in NCSU37, modified NCSU37, and NCSU37 containing MU following insemination was also examined, in order to ascertain a relationship between the size of the perivitelline space and the incidence of polyspermy. The perivitelline space could barely be seen in 70 \uff05 (21/30) of oocytes immediately after collection from antral follicles and could not be seen in 30 \uff05 of the oocytes. The mean size of the perivitelline space was 0.63 \u03bcm in oocytes immediately after collection from antral follicles and significantly increased to 4.38 and 4.58 \u03bcm at 32 and 44 hrs after culture. In DOs matured in modified NCSU37 with decreased concentration of NaCl, the size of perivitelline space (6.25 \u03bcm) was significantly larger than 4.93 \u03bcm in DOs cultured in NCSU37. The size of perivitelline space was significantly smaller in DOs cultured in NCSU37 containing MU (4.02 \u03bcm) than in those cultured in NCSU37 without MU (4.69 \u03bcm). DOs matured in NCSU37, modified NCSU37 with decreased concentration of NaCl, NCSU37 containing MU, NSCU37 containing no MU showed the maturation rate of 53.6, 56.7, 47.8 and 51.9\uff05 , respectively, and the fertilization rate after insemination of 82.5, 80.9, 88.1 and 85.7\uff05 , respectively. There were no differences in either the maturation rate or the fertilization rate seen among these four culture media. On the other hand, the incidence of polyspermy of DOs matured in modified NCSU37 with decreased concentration of NaCl was 32.7 \uff05 , which was significantly lower than 51.9 \uff05 of DOs matured in NCSU37. The incidence of polyspermy was significantly higher in DOs matured in NCSU37 containing MU (70.3 \uff05 ) than in control DOs matured in NCSU37 without MU (47.6 \uff05 ). From these findings, it was confirmed that the higher incidence of polyspermy is closely related to the smaller perivitelline space in porcine oocytes.", "subitem_description_type": "Abstract"}]}, "item_7_full_name_3": {"attribute_name": "\u8457\u8005\u5225\u540d", "attribute_value_mlt": [{"nameIdentifiers": [{"nameIdentifier": "162750", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "Jinbo, Yukihiro"}]}, {"nameIdentifiers": [{"nameIdentifier": "162751", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "Niimura, Sueo"}]}]}, "item_7_publisher_7": {"attribute_name": "\u51fa\u7248\u8005", "attribute_value_mlt": [{"subitem_publisher": "\u65b0\u6f5f\u5927\u5b66\u8fb2\u5b66\u90e8"}]}, "item_7_select_19": {"attribute_name": "\u8457\u8005\u7248\u30d5\u30e9\u30b0", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_7_source_id_11": {"attribute_name": "\u66f8\u8a8c\u30ec\u30b3\u30fc\u30c9ID", "attribute_value_mlt": [{"subitem_source_identifier": "AN00183393", "subitem_source_identifier_type": "NCID"}]}, "item_7_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "03858634", "subitem_source_identifier_type": "ISSN"}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "\u795e\u4fdd, \u5e78\u5f18"}], "nameIdentifiers": [{"nameIdentifier": "162748", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "\u65b0\u6751, \u672b\u96c4"}], "nameIdentifiers": [{"nameIdentifier": "162749", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-08-20"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "65(1)_23-30.pdf", "filesize": [{"value": "849.6 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 849600.0, "url": {"label": "65(1)_23-30.pdf", "url": "https://niigata-u.repo.nii.ac.jp/record/28314/files/65(1)_23-30.pdf"}, "version_id": "e4f1c0dd-b008-41c7-b5c3-629e9046935d"}]}, "item_keyword": {"attribute_name": "\u30ad\u30fc\u30ef\u30fc\u30c9", "attribute_value_mlt": [{"subitem_subject": "\u30d6\u30bf\u88f8\u5316\u5375\u6bcd\u7d30\u80de", "subitem_subject_scheme": "Other"}, {"subitem_subject": "\u56f2\u5375\u8154\u306e\u5927\u304d\u3055", "subitem_subject_scheme": "Other"}, {"subitem_subject": "\u591a\u7cbe\u5b50\u4fb5\u5165\u306e\u983b\u5ea6", "subitem_subject_scheme": "Other"}, {"subitem_subject": "denuded porcine oocyte", "subitem_subject_scheme": "Other"}, {"subitem_subject": "size of perivitelline space", "subitem_subject_scheme": "Other"}, {"subitem_subject": "polyspermy", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "jpn"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "\u57f9\u990a\u3057\u305f\u30d6\u30bf\u306e\u88f8\u5316\u5375\u6bcd\u7d30\u80de\u306e\u56f2\u5375\u8154\u306e\u5927\u304d\u3055\u3068\u5a92\u7cbe\u5f8c\u306e\u591a\u7cbe\u5b50\u4fb5\u5165\u306e\u983b\u5ea6", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "\u57f9\u990a\u3057\u305f\u30d6\u30bf\u306e\u88f8\u5316\u5375\u6bcd\u7d30\u80de\u306e\u56f2\u5375\u8154\u306e\u5927\u304d\u3055\u3068\u5a92\u7cbe\u5f8c\u306e\u591a\u7cbe\u5b50\u4fb5\u5165\u306e\u983b\u5ea6"}, {"subitem_title": "\u57f9\u990a\u3057\u305f\u30d6\u30bf\u306e\u88f8\u5316\u5375\u6bcd\u7d30\u80de\u306e\u56f2\u5375\u8154\u306e\u5927\u304d\u3055\u3068\u5a92\u7cbe\u5f8c\u306e\u591a\u7cbe\u5b50\u4fb5\u5165\u306e\u983b\u5ea6", "subitem_title_language": "en"}]}, "item_type_id": "7", "owner": "1", "path": ["453/456", "485/872/1583/1592"], "permalink_uri": "http://hdl.handle.net/10191/22573", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_value": "2013-05-29"}, "publish_date": "2013-05-29", "publish_status": "0", "recid": "28314", "relation": {}, "relation_version_is_last": true, "title": ["\u57f9\u990a\u3057\u305f\u30d6\u30bf\u306e\u88f8\u5316\u5375\u6bcd\u7d30\u80de\u306e\u56f2\u5375\u8154\u306e\u5927\u304d\u3055\u3068\u5a92\u7cbe\u5f8c\u306e\u591a\u7cbe\u5b50\u4fb5\u5165\u306e\u983b\u5ea6"], "weko_shared_id": null}