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In addition, the size of the perivitelline space of DOs cultured in modified NCSU37 with decreased concentration of NaCl and those cultured in NCSU37 containing 4-methylumbelliferone (MU), an inhibitor of hyaluronic acid synthase, was observed. The incidence of polyspermy in DOs matured in NCSU37, modified NCSU37, and NCSU37 containing MU following insemination was also examined, in order to ascertain a relationship between the size of the perivitelline space and the incidence of polyspermy. The perivitelline space could barely be seen in 70 % (21/30) of oocytes immediately after collection from antral follicles and could not be seen in 30 % of the oocytes. The mean size of the perivitelline space was 0.63 μm in oocytes immediately after collection from antral follicles and significantly increased to 4.38 and 4.58 μm at 32 and 44 hrs after culture. In DOs matured in modified NCSU37 with decreased concentration of NaCl, the size of perivitelline space (6.25 μm) was significantly larger than 4.93 μm in DOs cultured in NCSU37. The size of perivitelline space was significantly smaller in DOs cultured in NCSU37 containing MU (4.02 μm) than in those cultured in NCSU37 without MU (4.69 μm). DOs matured in NCSU37, modified NCSU37 with decreased concentration of NaCl, NCSU37 containing MU, NSCU37 containing no MU showed the maturation rate of 53.6, 56.7, 47.8 and 51.9% , respectively, and the fertilization rate after insemination of 82.5, 80.9, 88.1 and 85.7% , respectively. There were no differences in either the maturation rate or the fertilization rate seen among these four culture media. On the other hand, the incidence of polyspermy of DOs matured in modified NCSU37 with decreased concentration of NaCl was 32.7 % , which was significantly lower than 51.9 % of DOs matured in NCSU37. The incidence of polyspermy was significantly higher in DOs matured in NCSU37 containing MU (70.3 % ) than in control DOs matured in NCSU37 without MU (47.6 % ). 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  1. 0 資料タイプ別
  2. 03 紀要論文
  1. 070 農学部
  2. 20 紀要
  3. 01 新潟大学農学部研究報告
  4. 第65巻第1号

培養したブタの裸化卵母細胞の囲卵腔の大きさと媒精後の多精子侵入の頻度

http://hdl.handle.net/10191/22573
http://hdl.handle.net/10191/22573
50f5b830-3795-4849-9c4f-938050f270f8
名前 / ファイル ライセンス アクション
65(1)_23-30.pdf 65(1)_23-30.pdf (849.6 kB)
Item type 紀要論文 / Departmental Bulletin Paper(1)
公開日 2013-05-29
タイトル
タイトル 培養したブタの裸化卵母細胞の囲卵腔の大きさと媒精後の多精子侵入の頻度
タイトル
言語 en
タイトル 培養したブタの裸化卵母細胞の囲卵腔の大きさと媒精後の多精子侵入の頻度
言語
言語 jpn
キーワード
主題Scheme Other
主題 ブタ裸化卵母細胞
キーワード
主題Scheme Other
主題 囲卵腔の大きさ
キーワード
主題Scheme Other
主題 多精子侵入の頻度
キーワード
主題Scheme Other
主題 denuded porcine oocyte
キーワード
主題Scheme Other
主題 size of perivitelline space
キーワード
主題Scheme Other
主題 polyspermy
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ departmental bulletin paper
その他のタイトル
その他のタイトル Size of the Perivitelline Space of Denuded Porcine Oocytes and Incidence of Polyspermy following Insemination
著者 神保, 幸弘

× 神保, 幸弘

WEKO 162748

神保, 幸弘

Search repository
新村, 末雄

× 新村, 末雄

WEKO 162749

新村, 末雄

Search repository
著者別名
識別子 162750
識別子Scheme WEKO
姓名 Jinbo, Yukihiro
著者別名
識別子 162751
識別子Scheme WEKO
姓名 Niimura, Sueo
抄録
内容記述タイプ Abstract
内容記述 NCSU37で培養したブタの裸化卵母細胞について、成熟に伴う囲卵腔の大きさの変化を経時的に観察した。また、NaCl濃度を低くした低NaCl-NCSU37およびヒアルロン酸の合成阻害剤である4-メチルウンベリフェロン(MU)を添加したNCSU37で培養した裸化卵母細胞についても、成熟後の囲卵腔の大きさと媒精後の多精子侵入の頻度を観察した。採取直後の卵母細胞30個において、9個には囲卵腔はみられなかったが、21個には囲卵腔が観察され、卵母細胞30個の囲卵腔の平均値は0.63μmであった。囲卵腔は、培養8時間以降拡大したが、培養後22時間までの卵母細胞では採取直後のものと有意な相違はなかった。一方囲卵腔は、培養32時間以降有意に拡大し、培養44時間後では4.58μm になった。一方、各種培養液で成熟させた卵母細胞において、囲卵腔は、低NaCl-NCSU37で培養したものでは6.25μmであり、対照のNCSU37で培養したものの4.93μmに比べて有意に大きかった。また、MU処置した卵母細胞の囲卵腔は4.02μmであり、対照の卵母細胞の4.69μmに比べて有意に小さかった。受精率は、各種培養液で成熟させた卵母細胞の間で相違なかったが、多精子侵入の頻度は、低NaCl-NCSU37で培養した卵母細胞に比べ、NCSU37で培養した卵母細胞では有意に高いとともに、MU処置卵母細胞では、対照の卵母細胞に比べて有意に高かった。以上の結果から、囲卵腔の大きさと多精子侵入の頻度との間には密接な関係のあることが推察された。
抄録
内容記述タイプ Abstract
内容記述 The size of the perivitelline space was measured in porcine oocytes without cumulus cells (DOs) cultured in NCSU37. In addition, the size of the perivitelline space of DOs cultured in modified NCSU37 with decreased concentration of NaCl and those cultured in NCSU37 containing 4-methylumbelliferone (MU), an inhibitor of hyaluronic acid synthase, was observed. The incidence of polyspermy in DOs matured in NCSU37, modified NCSU37, and NCSU37 containing MU following insemination was also examined, in order to ascertain a relationship between the size of the perivitelline space and the incidence of polyspermy. The perivitelline space could barely be seen in 70 % (21/30) of oocytes immediately after collection from antral follicles and could not be seen in 30 % of the oocytes. The mean size of the perivitelline space was 0.63 μm in oocytes immediately after collection from antral follicles and significantly increased to 4.38 and 4.58 μm at 32 and 44 hrs after culture. In DOs matured in modified NCSU37 with decreased concentration of NaCl, the size of perivitelline space (6.25 μm) was significantly larger than 4.93 μm in DOs cultured in NCSU37. The size of perivitelline space was significantly smaller in DOs cultured in NCSU37 containing MU (4.02 μm) than in those cultured in NCSU37 without MU (4.69 μm). DOs matured in NCSU37, modified NCSU37 with decreased concentration of NaCl, NCSU37 containing MU, NSCU37 containing no MU showed the maturation rate of 53.6, 56.7, 47.8 and 51.9% , respectively, and the fertilization rate after insemination of 82.5, 80.9, 88.1 and 85.7% , respectively. There were no differences in either the maturation rate or the fertilization rate seen among these four culture media. On the other hand, the incidence of polyspermy of DOs matured in modified NCSU37 with decreased concentration of NaCl was 32.7 % , which was significantly lower than 51.9 % of DOs matured in NCSU37. The incidence of polyspermy was significantly higher in DOs matured in NCSU37 containing MU (70.3 % ) than in control DOs matured in NCSU37 without MU (47.6 % ). From these findings, it was confirmed that the higher incidence of polyspermy is closely related to the smaller perivitelline space in porcine oocytes.
書誌情報 新潟大学農学部研究報告
en : 新潟大学農学部研究報告

巻 65, 号 1, p. 23-30, 発行日 2012-09
出版者
出版者 新潟大学農学部
ISSN
収録物識別子タイプ ISSN
収録物識別子 03858634
書誌レコードID
収録物識別子タイプ NCID
収録物識別子 AN00183393
著者版フラグ
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