{"_buckets": {"deposit": "f0cbab6a-1d2a-4a36-a650-81a9ac4a609a"}, "_deposit": {"id": "28304", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "28304"}, "status": "published"}, "_oai": {"id": "oai:niigata-u.repo.nii.ac.jp:00028304", "sets": ["456", "1591"]}, "item_7_alternative_title_1": {"attribute_name": "その他のタイトル", "attribute_value_mlt": [{"subitem_alternative_title": "Brassica oleraceaにおける近交系統の純度検定に最適なDNAマーカーのスクリーニング"}]}, "item_7_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2013-03", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "2", "bibliographicPageEnd": "147", "bibliographicPageStart": "137", "bibliographicVolumeNumber": "65", "bibliographic_titles": [{"bibliographic_title": "新潟大学農学部研究報告"}, {"bibliographic_title": "新潟大学農学部研究報告", "bibliographic_titleLang": "en"}]}]}, "item_7_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Generating inbred lines is the first step in the breeding of F_1 hybrid cultivars in plants. It is generally considered that selfing for more than 5 or 6 generations is necessary for generating genetically identical inbred lines. DNA-marker based purity test of inbred lines is a reliable tool to confirm their genetic homogeneity, but few DNA markers, which are suitable for purity test of inbred lines, are reported in Brassica oleracea. In this study, we assessed 175 SSR primer sets to find out the DNA markers, which can detect the polymorphism between parental lines of F_1 hybrid cultivar. The F_2 plants derived from the F_1 hybrid cultivar of cabbage YR Kinshukyoryoku 152 was used to identify polymorphisms because the F_1 hybrid contains both of the parental genomes. The segregation of the parental genomes in the F_2 population allows detecting the nucleotide sequence differences between the individuals that can be traced back to the parental inbred lines of F_1 hybrid. Twelve primer sets showed polymorphism among F_2 plants, indicating that these DNA markers could be useful for purity test.", "subitem_description_type": "Abstract"}, {"subitem_description": "近交系統の作成は、植物のF_1品種の育成過程の最初の段階である。遺伝的に均一な近交系統の育成には５、６世代以上の自殖が必要であると考えられている。DNA マーカーを利用した近交系の純度検定は信頼性が高いが、B. oleracea では、純度検定に有効なDNAマーカーの報告は少ない。本研究では、F_1品種の両親系統間の多型を見分けることができるDNA マーカーを見出すために、175のSSR プライマー対を試した。F_2集団では、両親系統のゲノムが分離することから、両親系統の塩基配列の多型を追跡することが可能となる。そこで、材料にはキャベツの市販F_1品種であるYR 錦秋協力152のF_2個体を用いた。12のDNA マーカーでF_2集団の個体間で多型が見られたことから、これらのマーカーは純度検定に有効的であると考えられた。", "subitem_description_type": "Abstract"}]}, "item_7_full_name_3": {"attribute_name": "著者別名", "attribute_value_mlt": [{"nameIdentifiers": [{"nameIdentifier": "162683", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "富田, 寛也"}]}, {"nameIdentifiers": [{"nameIdentifier": "162684", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "清水, 元樹"}]}, {"nameIdentifiers": [{"nameIdentifier": "162685", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "久米, 千織"}]}, {"nameIdentifiers": [{"nameIdentifier": "162686", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "川辺, 隆大"}]}, {"nameIdentifiers": [{"nameIdentifier": "162687", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "長部, 謙二"}]}, {"nameIdentifiers": [{"nameIdentifier": "162688", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "岡崎, 桂一"}]}, {"nameIdentifiers": [{"nameIdentifier": "162689", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "藤本, 龍"}]}]}, "item_7_publisher_7": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "新潟大学農学部"}]}, "item_7_select_19": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_7_source_id_11": {"attribute_name": "書誌レコードID", "attribute_value_mlt": [{"subitem_source_identifier": "AN00183393", "subitem_source_identifier_type": "NCID"}]}, "item_7_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "03858634", "subitem_source_identifier_type": "ISSN"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Tomita, Hiroya"}], "nameIdentifiers": [{"nameIdentifier": "162676", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Shimizu, Motoki"}], "nameIdentifiers": [{"nameIdentifier": "162677", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kume, Chiori"}], "nameIdentifiers": [{"nameIdentifier": "162678", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kawanabe, Takahiro"}], "nameIdentifiers": [{"nameIdentifier": "162679", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Osabe, Kenji"}], "nameIdentifiers": [{"nameIdentifier": "162680", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Okazaki, Keiichi"}], "nameIdentifiers": [{"nameIdentifier": "162681", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Fujimoto, Ryo"}], "nameIdentifiers": [{"nameIdentifier": "162682", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-08-20"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "65(2)_137-147.pdf", "filesize": [{"value": "712.4 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 712400.0, "url": {"label": "65(2)_137-147.pdf", "url": "https://niigata-u.repo.nii.ac.jp/record/28304/files/65(2)_137-147.pdf"}, "version_id": "a0f7009e-8897-4ab4-a22c-2c287af8ed4c"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "Purity test 純度検査", "subitem_subject_scheme": "Other"}, {"subitem_subject": "inbred line 近交系", "subitem_subject_scheme": "Other"}, {"subitem_subject": "simple sequence repeats (SSR) primers SSRプライマー", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Brassica oleracea", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Screening of DNA markers suitable for purity test of inbred lines in Brassica oleracea", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Screening of DNA markers suitable for purity test of inbred lines in Brassica oleracea"}, {"subitem_title": "Screening of DNA markers suitable for purity test of inbred lines in Brassica oleracea", "subitem_title_language": "en"}]}, "item_type_id": "7", "owner": "1", "path": ["456", "1591"], "permalink_uri": "http://hdl.handle.net/10191/22571", "pubdate": {"attribute_name": "公開日", "attribute_value": "2013-05-29"}, "publish_date": "2013-05-29", "publish_status": "0", "recid": "28304", "relation": {}, "relation_version_is_last": true, "title": ["Screening of DNA markers suitable for purity test of inbred lines in Brassica oleracea"], "weko_shared_id": null}