{"_buckets": {"deposit": "f0cbab6a-1d2a-4a36-a650-81a9ac4a609a"}, "_deposit": {"id": "28304", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "28304"}, "status": "published"}, "_oai": {"id": "oai:niigata-u.repo.nii.ac.jp:00028304"}, "item_7_alternative_title_1": {"attribute_name": "\u305d\u306e\u4ed6\u306e\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_alternative_title": "Brassica oleracea\u306b\u304a\u3051\u308b\u8fd1\u4ea4\u7cfb\u7d71\u306e\u7d14\u5ea6\u691c\u5b9a\u306b\u6700\u9069\u306aDNA\u30de\u30fc\u30ab\u30fc\u306e\u30b9\u30af\u30ea\u30fc\u30cb\u30f3\u30b0"}]}, "item_7_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2013-03", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "2", "bibliographicPageEnd": "147", "bibliographicPageStart": "137", "bibliographicVolumeNumber": "65", "bibliographic_titles": [{"bibliographic_title": "\u65b0\u6f5f\u5927\u5b66\u8fb2\u5b66\u90e8\u7814\u7a76\u5831\u544a"}, {"bibliographic_title": "\u65b0\u6f5f\u5927\u5b66\u8fb2\u5b66\u90e8\u7814\u7a76\u5831\u544a", "bibliographic_titleLang": "en"}]}]}, "item_7_description_4": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "Generating inbred lines is the first step in the breeding of F_1 hybrid cultivars in plants. It is generally considered that selfing for more than 5 or 6 generations is necessary for generating genetically identical inbred lines. DNA-marker based purity test of inbred lines is a reliable tool to confirm their genetic homogeneity, but few DNA markers, which are suitable for purity test of inbred lines, are reported in Brassica oleracea. In this study, we assessed 175 SSR primer sets to find out the DNA markers, which can detect the polymorphism between parental lines of F_1 hybrid cultivar. The F_2 plants derived from the F_1 hybrid cultivar of cabbage YR Kinshukyoryoku 152 was used to identify polymorphisms because the F_1 hybrid contains both of the parental genomes. The segregation of the parental genomes in the F_2 population allows detecting the nucleotide sequence differences between the individuals that can be traced back to the parental inbred lines of F_1 hybrid. Twelve primer sets showed polymorphism among F_2 plants, indicating that these DNA markers could be useful for purity test.", "subitem_description_type": "Abstract"}, {"subitem_description": "\u8fd1\u4ea4\u7cfb\u7d71\u306e\u4f5c\u6210\u306f\u3001\u690d\u7269\u306eF_1\u54c1\u7a2e\u306e\u80b2\u6210\u904e\u7a0b\u306e\u6700\u521d\u306e\u6bb5\u968e\u3067\u3042\u308b\u3002\u907a\u4f1d\u7684\u306b\u5747\u4e00\u306a\u8fd1\u4ea4\u7cfb\u7d71\u306e\u80b2\u6210\u306b\u306f\uff15\u3001\uff16\u4e16\u4ee3\u4ee5\u4e0a\u306e\u81ea\u6b96\u304c\u5fc5\u8981\u3067\u3042\u308b\u3068\u8003\u3048\u3089\u308c\u3066\u3044\u308b\u3002DNA \u30de\u30fc\u30ab\u30fc\u3092\u5229\u7528\u3057\u305f\u8fd1\u4ea4\u7cfb\u306e\u7d14\u5ea6\u691c\u5b9a\u306f\u4fe1\u983c\u6027\u304c\u9ad8\u3044\u304c\u3001B. oleracea 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"nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Fujimoto, Ryo"}], "nameIdentifiers": [{"nameIdentifier": "162682", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-08-20"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "65(2)_137-147.pdf", "filesize": [{"value": "712.4 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 712400.0, "url": {"label": "65(2)_137-147.pdf", "url": "https://niigata-u.repo.nii.ac.jp/record/28304/files/65(2)_137-147.pdf"}, "version_id": "a0f7009e-8897-4ab4-a22c-2c287af8ed4c"}]}, "item_keyword": {"attribute_name": "\u30ad\u30fc\u30ef\u30fc\u30c9", "attribute_value_mlt": [{"subitem_subject": "Purity test \u7d14\u5ea6\u691c\u67fb", "subitem_subject_scheme": "Other"}, {"subitem_subject": "inbred line \u8fd1\u4ea4\u7cfb", "subitem_subject_scheme": "Other"}, {"subitem_subject": "simple sequence repeats (SSR) primers SSR\u30d7\u30e9\u30a4\u30de\u30fc", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Brassica oleracea", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Screening of DNA markers suitable for purity test of inbred lines in Brassica oleracea", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "Screening of DNA markers suitable for purity test of inbred lines in Brassica oleracea"}, {"subitem_title": "Screening of DNA markers suitable for purity test of inbred lines in Brassica oleracea", "subitem_title_language": "en"}]}, "item_type_id": "7", "owner": "1", "path": ["453/456", "485/872/1583/1591"], "permalink_uri": "http://hdl.handle.net/10191/22571", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_value": "2013-05-29"}, "publish_date": "2013-05-29", "publish_status": "0", "recid": "28304", "relation": {}, "relation_version_is_last": true, "title": ["Screening of DNA markers suitable for purity test of inbred lines in Brassica oleracea"], "weko_shared_id": null}