{"_buckets": {"deposit": "5ec5f805-1ece-4201-9906-186510a09dba"}, "_deposit": {"id": "28277", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "28277"}, "status": "published"}, "_oai": {"id": "oai:niigata-u.repo.nii.ac.jp:00028277", "sets": ["456", "1588"]}, "item_7_alternative_title_1": {"attribute_name": "その他のタイトル", "attribute_value_mlt": [{"subitem_alternative_title": "ウシ骨格筋由来デコリンの単離・精製ならびに高圧下における構造変化"}]}, "item_7_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2014-09", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "1", "bibliographicPageEnd": "48", "bibliographicPageStart": "43", "bibliographicVolumeNumber": "67", "bibliographic_titles": [{"bibliographic_title": "新潟大学農学部研究報告"}, {"bibliographic_title": "新潟大学農学部研究報告", "bibliographic_titleLang": "en"}]}]}, "item_7_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "High hydrostatic pressure induces a weakening of intramuscular connective tissue, which is mainly composed of collagen. Decorin, a small proteoglycan, binds to and stabilizes collagen fibrils. It has been suggested that the weakening of intramuscular connective tissue may result from alteration of the decorin-collagen interaction due to structural changes of the decorin molecule. In the present study, decorin was isolated and purified from bovine skeletal muscle by successive steps of an extraction with 4 M guanidine hydrochloride, CsCl density gradient ultracentrifugation, DEAE-cellulose ion-exchange chromatography, and Sepharose CL-6B gel filtration. The isolated decorin possessed an average molecular mass of 100 kDa and contained a core protein mass of 48 kDa by SDS-PAGE. The structural changes in decorin from bovine skeletal muscle were investigated by measuring fluorescence spectra under high pressure. Fluorescence spectra of purified decorin indicated that the tertiary structure of decorin was altered reversibly under high pressure up to 400 MPa.", "subitem_description_type": "Abstract"}, {"subitem_description": "高圧処理は主にコラーゲンから構成される筋肉内結合組織の脆弱化を引き起こす。デコリンは小型プロテオグリカンの一つであり、アーチ型構造のコアタンパク質と1本のグリコサミノグリカン鎖を持つ。デコリンの役割の一つとしてコラーゲン細線維に結合し、その構造を束ねるようにして安定化させることが知られている。当研究室では、結合組織の脆弱化は高圧処理がデコリン分子の構造に何らかの影響を及ぼし、デコリンとコラーゲンの相互作用が変化することで生じると推測している。本研究ではまず、ウシ肩ロース肉から4 M グアニジン塩酸溶液によりプロテオグリカンを抽出した後、塩化セシウム密度勾配遠心分離によりプロテオグリカンを単離した。その後、DEAE- セルロースイオン交換クロマトグラフィーとSepharose CL-6B ゲルろ過クロマトグラフィーにより精製した。デコリンは通常、約48 kDa のコアタンパク質を持ち、グリコサミノグリカン鎖と合わせると全体の分子質量は約100 kDa になる。 SDS-PAGE の結果からこれらの特徴を有していたため、単離・精製の過程を経て得られたものをウシ骨格筋由来精製デコリン試料とし、高圧下での蛍光スペクトル分析に用いた。精製デコリンの高圧下での蛍光スペクトルは400 MPa までの圧力で可逆的な三次構造の変化を引き起こすことを示した。", "subitem_description_type": "Abstract"}]}, "item_7_full_name_3": {"attribute_name": "著者別名", "attribute_value_mlt": [{"nameIdentifiers": [{"nameIdentifier": "162465", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "細野, 匠"}]}, {"nameIdentifiers": [{"nameIdentifier": "162466", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "大竹, 祐希"}]}, {"nameIdentifiers": [{"nameIdentifier": "162467", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "小茂田, 嵩人"}]}, {"nameIdentifiers": [{"nameIdentifier": "162468", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "杉山, 稔恵"}]}, {"nameIdentifiers": [{"nameIdentifier": "162469", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "西海, 理之"}]}]}, "item_7_publisher_7": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "新潟大学農学部"}]}, "item_7_select_19": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_7_source_id_11": {"attribute_name": "書誌レコードID", "attribute_value_mlt": [{"subitem_source_identifier": "AN00183393", "subitem_source_identifier_type": "NCID"}]}, "item_7_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "03858634", "subitem_source_identifier_type": "ISSN"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Hosono, Takumi"}], "nameIdentifiers": [{"nameIdentifier": "162460", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Otake, Yuki"}], "nameIdentifiers": [{"nameIdentifier": "162461", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Komoda, Takahito"}], "nameIdentifiers": [{"nameIdentifier": "162462", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Sugiyama, Toshie"}], "nameIdentifiers": [{"nameIdentifier": "162463", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nishiumi, Tadayuki"}], "nameIdentifiers": [{"nameIdentifier": "162464", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-08-20"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "67(1)_43-48.pdf", "filesize": [{"value": "1.7 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 1700000.0, "url": {"label": "67(1)_43-48.pdf", "url": "https://niigata-u.repo.nii.ac.jp/record/28277/files/67(1)_43-48.pdf"}, "version_id": "6baa4928-8747-4bec-99e8-613abfe51279"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "Decorin", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Collagen", "subitem_subject_scheme": "Other"}, {"subitem_subject": "High pressure", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Meat tenderization", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Proteoglycan", "subitem_subject_scheme": "Other"}, {"subitem_subject": "デコリン", "subitem_subject_scheme": "Other"}, {"subitem_subject": "コラーゲン", "subitem_subject_scheme": "Other"}, {"subitem_subject": "高圧", "subitem_subject_scheme": "Other"}, {"subitem_subject": "食肉軟化", "subitem_subject_scheme": "Other"}, {"subitem_subject": "プロテオグリカン", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Isolation and purification of decorin from bovine skeletal muscle and its structural changes under high pressure", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Isolation and purification of decorin from bovine skeletal muscle and its structural changes under high pressure"}, {"subitem_title": "Isolation and purification of decorin from bovine skeletal muscle and its structural changes under high pressure", "subitem_title_language": "en"}]}, "item_type_id": "7", "owner": "1", "path": ["456", "1588"], "permalink_uri": "http://hdl.handle.net/10191/30056", "pubdate": {"attribute_name": "公開日", "attribute_value": "2014-10-06"}, "publish_date": "2014-10-06", "publish_status": "0", "recid": "28277", "relation": {}, "relation_version_is_last": true, "title": ["Isolation and purification of decorin from bovine skeletal muscle and its structural changes under high pressure"], "weko_shared_id": null}