{"_buckets": {"deposit": "5ec5f805-1ece-4201-9906-186510a09dba"}, "_deposit": {"id": "28277", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "28277"}, "status": "published"}, "_oai": {"id": "oai:niigata-u.repo.nii.ac.jp:00028277"}, "item_7_alternative_title_1": {"attribute_name": "\u305d\u306e\u4ed6\u306e\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_alternative_title": "\u30a6\u30b7\u9aa8\u683c\u7b4b\u7531\u6765\u30c7\u30b3\u30ea\u30f3\u306e\u5358\u96e2\u30fb\u7cbe\u88fd\u306a\u3089\u3073\u306b\u9ad8\u5727\u4e0b\u306b\u304a\u3051\u308b\u69cb\u9020\u5909\u5316"}]}, "item_7_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2014-09", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "1", "bibliographicPageEnd": "48", "bibliographicPageStart": "43", "bibliographicVolumeNumber": "67", "bibliographic_titles": [{"bibliographic_title": "\u65b0\u6f5f\u5927\u5b66\u8fb2\u5b66\u90e8\u7814\u7a76\u5831\u544a"}, {"bibliographic_title": "\u65b0\u6f5f\u5927\u5b66\u8fb2\u5b66\u90e8\u7814\u7a76\u5831\u544a", "bibliographic_titleLang": "en"}]}]}, "item_7_description_4": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "High hydrostatic pressure induces a weakening of intramuscular connective tissue, which is mainly composed of collagen. Decorin, a small proteoglycan, binds to and stabilizes collagen fibrils. It has been suggested that the weakening of intramuscular connective tissue may result from alteration of the decorin-collagen interaction due to structural changes of the decorin molecule. In the present study, decorin was isolated and purified from bovine skeletal muscle by successive steps of an extraction with 4 M guanidine hydrochloride, CsCl density gradient ultracentrifugation, DEAE-cellulose ion-exchange chromatography, and Sepharose CL-6B gel filtration. The isolated decorin possessed an average molecular mass of 100 kDa and contained a core protein mass of 48 kDa by SDS-PAGE. The structural changes in decorin from bovine skeletal muscle were investigated by measuring fluorescence spectra under high pressure. Fluorescence spectra of purified decorin indicated that the tertiary structure of decorin was altered reversibly under high pressure up to 400 MPa.", "subitem_description_type": "Abstract"}, {"subitem_description": 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M \u30b0\u30a2\u30cb\u30b8\u30f3\u5869\u9178\u6eb6\u6db2\u306b\u3088\u308a\u30d7\u30ed\u30c6\u30aa\u30b0\u30ea\u30ab\u30f3\u3092\u62bd\u51fa\u3057\u305f\u5f8c\u3001\u5869\u5316\u30bb\u30b7\u30a6\u30e0\u5bc6\u5ea6\u52fe\u914d\u9060\u5fc3\u5206\u96e2\u306b\u3088\u308a\u30d7\u30ed\u30c6\u30aa\u30b0\u30ea\u30ab\u30f3\u3092\u5358\u96e2\u3057\u305f\u3002\u305d\u306e\u5f8c\u3001DEAE- \u30bb\u30eb\u30ed\u30fc\u30b9\u30a4\u30aa\u30f3\u4ea4\u63db\u30af\u30ed\u30de\u30c8\u30b0\u30e9\u30d5\u30a3\u30fc\u3068Sepharose CL-6B \u30b2\u30eb\u308d\u904e\u30af\u30ed\u30de\u30c8\u30b0\u30e9\u30d5\u30a3\u30fc\u306b\u3088\u308a\u7cbe\u88fd\u3057\u305f\u3002\u30c7\u30b3\u30ea\u30f3\u306f\u901a\u5e38\u3001\u7d0448 kDa \u306e\u30b3\u30a2\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u6301\u3061\u3001\u30b0\u30ea\u30b3\u30b5\u30df\u30ce\u30b0\u30ea\u30ab\u30f3\u9396\u3068\u5408\u308f\u305b\u308b\u3068\u5168\u4f53\u306e\u5206\u5b50\u8cea\u91cf\u306f\u7d04100 kDa \u306b\u306a\u308b\u3002 SDS-PAGE 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{"subitem_subject": "High pressure", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Meat tenderization", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Proteoglycan", "subitem_subject_scheme": "Other"}, {"subitem_subject": "\u30c7\u30b3\u30ea\u30f3", "subitem_subject_scheme": "Other"}, {"subitem_subject": "\u30b3\u30e9\u30fc\u30b2\u30f3", "subitem_subject_scheme": "Other"}, {"subitem_subject": "\u9ad8\u5727", "subitem_subject_scheme": "Other"}, {"subitem_subject": "\u98df\u8089\u8edf\u5316", "subitem_subject_scheme": "Other"}, {"subitem_subject": "\u30d7\u30ed\u30c6\u30aa\u30b0\u30ea\u30ab\u30f3", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Isolation and purification of decorin from bovine skeletal muscle and its structural changes under high pressure", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "Isolation and purification of decorin from bovine skeletal muscle and its structural changes under high pressure"}, {"subitem_title": "Isolation and purification of decorin from bovine skeletal muscle and its structural changes under high pressure", "subitem_title_language": "en"}]}, "item_type_id": "7", "owner": "1", "path": ["453/456", "485/872/1583/1588"], "permalink_uri": "http://hdl.handle.net/10191/30056", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_value": "2014-10-06"}, "publish_date": "2014-10-06", "publish_status": "0", "recid": "28277", "relation": {}, "relation_version_is_last": true, "title": ["Isolation and purification of decorin from bovine skeletal muscle and its structural changes under high pressure"], "weko_shared_id": null}