{"_buckets": {"deposit": "6efd9070-64d7-458c-8eca-f030bd7b6bfe"}, "_deposit": {"id": "2795", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "2795"}, "status": "published"}, "_oai": {"id": "oai:niigata-u.repo.nii.ac.jp:00002795", "sets": ["454", "487"]}, "item_5_alternative_title_1": {"attribute_name": "その他のタイトル", "attribute_value_mlt": [{"subitem_alternative_title": "Pollen Tube Growth and Seed Set in Stigmatic and Cut-style Pollinated Lilium longiflorum ‘Georgia’ Flowers as Influenced by Pre-pollination"}]}, "item_5_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "1996-06", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "1", "bibliographicPageEnd": "144", "bibliographicPageStart": "135", "bibliographicVolumeNumber": "65", "bibliographic_titles": [{"bibliographic_title": "園芸学会雑誌"}, {"bibliographic_title": "園芸学会雑誌", "bibliographic_titleLang": "en"}]}]}, "item_5_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "自家不和合性のテッポウユリ‘ジョージア’を用い，花柱切断受粉を行う場合の予備受粉が花粉管の生長および種子形成に及ぼす影響を調べた．\\r\\\\n1．‘花柱切断受粉法’では自家および交雑受粉に関係なく花粉管は受粉48時間後に花柱基部に到達した．予備受粉を行うと花粉管伸長が促進され，花粉管は受粉後24時間目に多くの個体で花柱基部まで達することがわかった．\\r\\\\n2．自家および交雑受粉において，花柱切断受粉および予備受粉は花粉管内の生殖核の分裂に影響した．受粉24時間後の観察では，‘柱頭受粉法’による交雑受粉（対照区）で観察した花粉管のうち，二つの生殖核と一つの栄養核（‘2S+V’）を有する花粉管は78%であった．一方，‘花柱切断受粉法’の交雑受粉で32%，自家受粉で9%であり，‘ひのもと’の花粉を予備受粉に使うと‘2S+V’の花粉管比率が増加した．\\r\\\\n3．‘柱頭受粉法’による交雑受粉（対照区）では，子房内に進入した花粉管は2列の胚珠間にある溝（胎座溝）を子房基部に向かって束となって伸長し，子房中央部付近には多くの花粉管が観察された．しかし，‘花柱切断受粉法’で子房内に入った花粉管は，‘柱頭受粉法’のものと比較するとその伸長方向は規則性を欠き，子房中央部付近での花粉管はまばらであった．\\r\\\\n4．‘柱頭受粉法’の交雑受粉（対照区）では，花粉管が侵入した胚珠は35%であったが，‘花柱切断受粉法’では6～8%で，予備受粉を行うことによりその率は向上した．\\r\\\\n5．‘花柱切断受粉法’による自家および交雑組み合わせで得られた種子はさく果あたり3粒と6粒であった．‘予備受粉+花柱切断受粉法’では‘ジョージア’の自家受粉では11粒，‘ジョージア’בひのもと’で23粒と優位に増加したが，‘柱頭受粉法’による交雑受粉（対照区）の94粒と比べかなり少なかった．", "subitem_description_type": "Abstract"}, {"subitem_description": "The present study was conducted to examine whether pre-pollination is effective in improving growth of pollen tubes and seed-sets of Lilium longiflorum \u0027Georgia\u0027 by comparing cut-style pollination (CSP) and stigmatic pollination (SP).\\r\\\\n1. Pollen tubes reached the basal end of the style by 48 hr in self-pollination of \u0027Georgia\u0027 and cross-pollination of \u0027Georgia\u0027×\u0027Hinomoto\u0027 by CSP. Using the pre-pollination technique in CSP enhanced the growth of pollen tubes both in self- and cross-pollination.\\r\\\\n2. Generative cell division of pollen tubes in the style was observed 24hr after pollination. In cross-pollination by SP, 78% of the pollen tubes had 2 sperm nuclei and 1 vegetative nucleus (\u00272S+V\u0027), while only 15% of these appeared in the self-combination. Using CSP, pollen tubes with \u00272S+V\u0027 were 32%in cross- and 9%in self-pollination. The percentages increased to 54% and 35%, respectively, when pollen of \u0027Hinomoto\u0027 was used in the pre-pollination treatment.\\r\\\\n3. In SP, numerous pollen tubes which penetrated the style grew in a bunch between two rows of ovules in an ovary ; only a few pollen tubes reached the ovules near the mid-region.\\r\\\\n4. Penetration of pollen tubes to the micropyle occurred in about 35% of the ovules in cross-pollination by SP and in less than 10% of these by CSP. Pre-pollination in CSP effectively increased the percentage of ovules with a pollen tube in the micropyle.\\r\\\\n5. Only 3 full-seeds per capsule in self- and 6 in cross-pollination were obtained by CSP, whereas 11 and 23 seeds per capsule, respectively, were obtained as the result of pre-pollination.", "subitem_description_type": "Abstract"}]}, "item_5_full_name_3": {"attribute_name": "著者別名", "attribute_value_mlt": [{"nameIdentifiers": [{"nameIdentifier": "39108", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "Li, Tong Hua"}]}, {"nameIdentifiers": [{"nameIdentifier": "39109", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "Niimi, Yoshiji"}]}, {"nameIdentifiers": [{"nameIdentifier": "39110", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "Nakano, Masaru"}]}]}, "item_5_publisher_7": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "園芸学会"}]}, "item_5_rights_15": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "園芸学会"}]}, "item_5_select_19": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_5_source_id_11": {"attribute_name": "書誌レコードID", "attribute_value_mlt": [{"subitem_source_identifier": "AN00025257", "subitem_source_identifier_type": "NCID"}]}, "item_5_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "00137626", "subitem_source_identifier_type": "ISSN"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "李, 同華"}], "nameIdentifiers": [{"nameIdentifier": "39105", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "新美, 芳二"}], "nameIdentifiers": [{"nameIdentifier": "39106", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "中野, 優"}], "nameIdentifiers": [{"nameIdentifier": "39107", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-07-30"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "65(1)_135-144.pdf", "filesize": [{"value": "4.1 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 4099999.9999999995, "url": {"label": "65(1)_135-144.pdf", "url": "https://niigata-u.repo.nii.ac.jp/record/2795/files/65(1)_135-144.pdf"}, "version_id": "6339a6f6-c8bf-4af5-a21e-2787707f49bb"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "jpn"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "花柱切断受粉法によるテッポウユリ‘ジョージア’の花粉管伸長と種子形成に及ぼす予備受粉の効果", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "花柱切断受粉法によるテッポウユリ‘ジョージア’の花粉管伸長と種子形成に及ぼす予備受粉の効果"}, {"subitem_title": "花柱切断受粉法によるテッポウユリ‘ジョージア’の花粉管伸長と種子形成に及ぼす予備受粉の効果", "subitem_title_language": "en"}]}, "item_type_id": "5", "owner": "1", "path": ["454", "487"], "permalink_uri": "http://hdl.handle.net/10191/6573", "pubdate": {"attribute_name": "公開日", "attribute_value": "2008-10-14"}, "publish_date": "2008-10-14", "publish_status": "0", "recid": "2795", "relation": {}, "relation_version_is_last": true, "title": ["花柱切断受粉法によるテッポウユリ‘ジョージア’の花粉管伸長と種子形成に及ぼす予備受粉の効果"], "weko_shared_id": null}