{"_buckets": {"deposit": "43f64459-61bf-47f9-a063-6063b3b6595b"}, "_deposit": {"id": "25933", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "25933"}, "status": "published"}, "_oai": {"id": "oai:niigata-u.repo.nii.ac.jp:00025933", "sets": ["456", "1326"]}, "item_7_alternative_title_1": {"attribute_name": "その他のタイトル", "attribute_value_mlt": [{"subitem_alternative_title": "A new sampling technique to obtain the elemental mapping of a bio-tissue section by means of the EPMA equipped with WDX"}]}, "item_7_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "1996-08", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "1", "bibliographicPageEnd": "37", "bibliographicPageStart": "29", "bibliographicVolumeNumber": "26", "bibliographic_titles": [{"bibliographic_title": "新潟歯学会雑誌"}, {"bibliographic_title": "新潟歯学会雑誌", "bibliographic_titleLang": "en"}]}]}, "item_7_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Electron Probe Microanalyzer (EPMA) is a powerful insrument for elemental analysis on a micron meter scale. In ordinary use, however, the samples to be applied are restricted to heat-stable materials, such as metals or ceramics, because irradiation of a finely focused electron beam generates intense heat in such a local area. Accordingly the elemental analysis of a bio-tissue section by EPMA was believed to be very difficult because such a section is very weak to heat and its elemental contents are extremely low. By using the carbon block with a polished mirror surface as a support and by slicing off an appropriate thick bio-section, the sample becomes to be very stable against the intense electron beam. As a result, mapping analysis of even low content element in bio-tissue can be obtained successfully. This new sampling technique can be applied to a study on the distribution of copper in the liver tissue in primary biliary cirrhosis (PBC) and other liver diseases. The results obtained are compared with an ordinary staining method and turned out to be pathologically valuable. In conclusion, this technique is found to be very useful for studying on the distribution of a cellular component.", "subitem_description_type": "Abstract"}, {"subitem_description": "X線マイクロアナライザー(以下EPMAと記す)は微小部分の元素分析では不可欠の装置で、材料研究で広く使用されている。しかしながら電子線を非常に細く絞って試料に照射するため、分析する部位が高温となり、従来は金属やセラミックスなどの耐熱性の試料に適用が限られてきた。生体組織切片は非常に熱に弱く、しかもそこに含まれている元素は一般に極めて低濃度のため、EPMAによる元素分析は非常に困難と考えられてきた。カーボンブロックを鏡面になるまで研磨し試料台とすること、および切片厚さを最適にすることで、生体組織切片でありながら、熱によるダメージを回避できた。この結果、強い電子線によるEPMA分析を可能にし、低濃度元素の二次元分布も観察できるようになった。この応用として、原発性胆汁性肝硬変における銅の肝組織および細胞内分布の検出に用い、染色法により検出された銅の分布と比較した場合、病理組織学的に利用価値の高いことが確認され、本研究で開発された試料作製法が極めて有用であることが分かった。", "subitem_description_type": "Abstract"}]}, "item_7_full_name_3": {"attribute_name": "著者別名", "attribute_value_mlt": [{"nameIdentifiers": [{"nameIdentifier": "153096", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "Kobayashi, Masayoshi"}]}, {"nameIdentifiers": [{"nameIdentifier": "153097", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "Watanabe, Kouichi"}]}, {"nameIdentifiers": [{"nameIdentifier": "153098", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "Miyakawa, Osamu"}]}]}, "item_7_publisher_7": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "新潟歯学会"}]}, "item_7_select_19": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_7_source_id_11": {"attribute_name": "書誌レコードID", "attribute_value_mlt": [{"subitem_source_identifier": "AN0018300X", "subitem_source_identifier_type": "NCID"}]}, "item_7_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "03850153", "subitem_source_identifier_type": "ISSN"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "小林, 正義"}], "nameIdentifiers": [{"nameIdentifier": "153093", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "渡辺, 孝一"}], "nameIdentifiers": [{"nameIdentifier": "153094", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "宮川, 修"}], "nameIdentifiers": [{"nameIdentifier": "153095", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-08-19"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "NS_26(1)_29-37.pdf", "filesize": [{"value": "5.9 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 5900000.0, "url": {"label": "NS_26(1)_29-37.pdf", "url": "https://niigata-u.repo.nii.ac.jp/record/25933/files/NS_26(1)_29-37.pdf"}, "version_id": "991b22aa-e0c4-4cae-8c37-fc7c20fa7eb7"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "EPMA", "subitem_subject_scheme": "Other"}, {"subitem_subject": "bio-tissue section", "subitem_subject_scheme": "Other"}, {"subitem_subject": "elemental mapping", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "jpn"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "波長分散型X線マイクロアナライザーにより生体組織切片の元素分布を得る試料作製法", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "波長分散型X線マイクロアナライザーにより生体組織切片の元素分布を得る試料作製法"}, {"subitem_title": "波長分散型X線マイクロアナライザーにより生体組織切片の元素分布を得る試料作製法", "subitem_title_language": "en"}]}, "item_type_id": "7", "owner": "1", "path": ["456", "1326"], "permalink_uri": "http://hdl.handle.net/10191/22960", "pubdate": {"attribute_name": "公開日", "attribute_value": "2013-08-21"}, "publish_date": "2013-08-21", "publish_status": "0", "recid": "25933", "relation": {}, "relation_version_is_last": true, "title": ["波長分散型X線マイクロアナライザーにより生体組織切片の元素分布を得る試料作製法"], "weko_shared_id": null}