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In ordinary use, however, the samples to be applied are restricted to heat-stable materials, such as metals or ceramics, because irradiation of a finely focused electron beam generates intense heat in such a local area. Accordingly the elemental analysis of a bio-tissue section by EPMA was believed to be very difficult because such a section is very weak to heat and its elemental contents are extremely low. By using the carbon block with a polished mirror surface as a support and by slicing off an appropriate thick bio-section, the sample becomes to be very stable against the intense electron beam. As a result, mapping analysis of even low content element in bio-tissue can be obtained successfully. This new sampling technique can be applied to a study on the distribution of copper in the liver tissue in primary biliary cirrhosis (PBC) and other liver diseases. The results obtained are compared with an ordinary staining method and turned out to be pathologically valuable. In conclusion, this technique is found to be very useful for studying on the distribution of a cellular component.", "subitem_description_type": "Abstract"}, {"subitem_description": 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