{"_buckets": {"deposit": "357cae05-1c0f-4c33-a899-f10c85efe14d"}, "_deposit": {"id": "25654", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "25654"}, "status": "published"}, "_oai": {"id": "oai:niigata-u.repo.nii.ac.jp:00025654"}, "item_7_alternative_title_1": {"attribute_name": "\u305d\u306e\u4ed6\u306e\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_alternative_title": "Phylogenetic Diversity of Enterococcus faecalis from Human Oral Cavities and Intestinal Tracts"}]}, "item_7_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2003-12", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "2", "bibliographicPageEnd": "23", "bibliographicPageStart": "17", "bibliographicVolumeNumber": "33", "bibliographic_titles": [{"bibliographic_title": "\u65b0\u6f5f\u6b6f\u5b66\u4f1a\u96d1\u8a8c"}, {"bibliographic_title": "\u65b0\u6f5f\u6b6f\u5b66\u4f1a\u96d1\u8a8c", "bibliographic_titleLang": "en"}]}]}, "item_7_description_4": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "Enterococcus Faecalis has been isolated frequently from human intestinal tracts. Also we have reported previously that these bacterial species has been occurred as predominant alkali resistant bacteria in human infected root canals. The aim of this study is to evaluate phylogenetic diversity of 16S rRNA of the human oral E. faecalis. In the present study, we used 9 strains of E. faecalis, ATCC 19433T, 4 strains isolated from human infected root canals and 4 strains isolated from human feces. From cells of these bacterial strains, genomic DNAs were extracted by using InstaGene Matrix and used as templates for amplification of 16S rDNA with universal primer sets in Polymerase Chain Reactions (PCR). For cycle sequence method of 16S rDNA sequence analysis, a Thermo Sequenase fluorescent labelled primer cycle sequencing kit was used with the 11 universal primer sets labeled with Cy-5, following the manufacture\u0027s protocol. Sequences of 16S rDNA were analyzed with a DNA sequencer. The segmented nucleotide sequences of 16S rDNA were connected using Seqman in LASERGENE computer program. Sequence similarity was analyzed by a clustal W method, which is programmed by Megalign in the LASERGENE computer program. 16S rDNA sequences of the E. faecalis reference strain (ATCC 19433T) was highly similar (98.4\uff5e100%) to those of the feces isolates and oral isolates, repectively. On the other hands, when the 16S rDNA sequences of oral isolates were compared to those of isolates from feces, these similarities were ranged widely and low (93.1\uff5e99.1%). These results of the present study has suggested that some of human oral E. faecalis may adapt to oral conditions and change genetically.", "subitem_description_type": "Abstract"}, {"subitem_description": "Enterococcus faecalis\u306f\u30d2\u30c8\u306e\u8178\u7ba1\u7d30\u83cc\u53e2\u306b\u5e83\u304f\u751f\u606f\u3057\u3066\u3044\u308b\u3002\u6211\u3005\u306f\u3053\u308c\u307e\u3067\u3001\u672c\u83cc\u7a2e\u304c\u611f\u67d3\u6839\u7ba1\u8c61\u7259\u8cea\u304b\u3089\u691c\u51fa\u3055\u308c\u308b\u3053\u3068\u3084\u3001\u5f37\u3044\u30a2\u30eb\u30ab\u30ea\u8010\u6027\u3092\u793a\u3059\u3053\u3068\u3092\u5831\u544a\u3057\u3066\u304d\u305f\uff61\u4eca\u56de\u306f\u3001\u751f\u606f\u306e\u5834\u306e\u9055\u3044\u306b\u3088\u308b\u907a\u4f1d\u5b66\u7684\u5909\u7570\u3092\u660e\u3089\u304b\u306b\u3059\u308b\u76ee\u7684\u3067\u3001\u611f\u67d3\u6839\u7ba1\u53ca\u3073\u8178\u7ba1\u5206\u96e2E.faecalis\u306b\u3064\u3044\u3066\u3001\u305d\u306e16S rRNA 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