WEKO3
アイテム
Flow Cytometric Analysis of Partially Synchronized Suspension Cultures of Hemerocallis hybrida
http://hdl.handle.net/10191/29856
http://hdl.handle.net/10191/2985642d8778a-6330-44ed-80ac-ce006f486070
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
18_3_229-231.pdf (239.6 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2014-09-10 | |||||
| タイトル | ||||||
| タイトル | Flow Cytometric Analysis of Partially Synchronized Suspension Cultures of Hemerocallis hybrida | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | flow cytometry | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Hemerocallis hybrida | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | mitotic index | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | synchronization of cell division | |||||
| 資源タイプ | ||||||
| 資源 | http://purl.org/coar/resource_type/c_6501 | |||||
| タイプ | journal article | |||||
| 著者 |
Saito, Hiroyuki
× Saito, Hiroyuki× Nakano, Masaru |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Cell division of suspension cultures of Hemerocallis hybrida was partially synchronized by treating with hydroxyurea (HU), a DNA synthesis inhibitor, followed by treatment with colchicine (COL), a spindle toxin, as the first step toward an efficient preparation of microprotoplasts. During COL treatment, suspension cells were analyzed for relative DNA contents using flow cytometry (FCM) as well as mitotic index (MI) under microscopy. MI reached a peak after 20h of COL treatment. In FCM analyses, histogram of asynchronous cultures showed three peaks (2C, 4C and 8C nuclei), and the numbers of both 4C and 8C nuclei gradually increased until 18h after the initiation of COL treatment. After 20h of COL treatment, the numbers of 4C and 8C nuclei transiently decreased, indicating that a large number of cells were at the prometaphase to metaphase stages. These results indicate that FCM analyses offer a simple and rapid means of preliminary examination for arresting cell-cycle at metaphase in partially synchronized suspension cultures of H. hybrida. | |||||
| 言語 | en | |||||
| 書誌情報 |
en : Plant biotechnology 巻 18, 号 3, p. 229-231, 発行日 2001-09 |
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| 出版者 | ||||||
| 出版者 | 日本植物細胞分子生物学会 | |||||
| 言語 | ja | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1342-4580 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA11250821 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | http://doi.org/10.5511/plantbiotechnology.18.229 | |||||
| 出版タイプ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| 著者版フラグ | ||||||
| 値 | publisher | |||||
