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Purification and characterization of golgi membrane-bound nucleoside diphosphatase from suspension-cultured cells of sycamore (acer pseudoplatanus L.)
http://hdl.handle.net/10191/17591
http://hdl.handle.net/10191/17591177622ff-ebc8-4d95-b937-f65c85971d6b
名前 / ファイル | ライセンス | アクション |
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2012-04-04 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Purification and characterization of golgi membrane-bound nucleoside diphosphatase from suspension-cultured cells of sycamore (acer pseudoplatanus L.) | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Cell culture | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Golgi membranes | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | IDPase | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | NDPase | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | sycamore | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
著者 |
Mikami, Satoshi
× Mikami, Satoshi× Suganuma, Ryo× Hori, Hidetaka× Mitsui, Toshiaki |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Inosine diphosphatase (IDPase) isoforms associated with Golgi membranes were studied in sycamore cell culture. These enzyme isoforms were solubilized with Triton X-100 and purified by chromatography using DEAE-Toyopearl and SOURCE-S columns. The isoforms were separated into two distinguishable fractions (peak 1 and 2) by SOURCE-S column chromatography. Furthermore the peak 1 contained at least two isoform bands detected by native-PAGE analysis. The apparent molecular sizes of these three isoforms were estimated by both gel filtration and SDS-PAGE to be 50 kDa, indicating that the Golgi membrane-bound IDPase has a monomeric structure. These IDPase isoforms required divalent cations (Ca2+, Mg2+, Co2+, Mn2+) for their hydrolyzing activity, and were inhibited by ATP. IDP, UDP, and GDP were effective substrates for these enzymes. It is clearly indicated that the sycamore Golgi membrane-bound IDPase is a nucleoside diphosphatase. | |||||
言語 | en | |||||
書誌情報 |
en : Plant biotechnology 巻 18, 号 4, p. 259-265, 発行日 2001-09 |
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出版者 | ||||||
言語 | ja | |||||
出版者 | 日本植物細胞分子生物学会 | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1342-4580 | |||||
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収録物識別子タイプ | NCID | |||||
収録物識別子 | AA11250821 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | http://doi.org/10.5511/plantbiotechnology.18.259 | |||||
著者版フラグ | ||||||
値 | publisher |