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Genetic engineering of nitrite reductase gene improves uptake and assimilation of nitrogen dioxide by Rhaphiolepis umbellata (Thunb.) Makino
http://hdl.handle.net/10191/29871
http://hdl.handle.net/10191/2987151d2344b-1409-402a-b4c5-a64fcaa50255
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
23_1_111-116.pdf (1.6 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2014-09-10 | |||||
| タイトル | ||||||
| タイトル | Genetic engineering of nitrite reductase gene improves uptake and assimilation of nitrogen dioxide by Rhaphiolepis umbellata (Thunb.) Makino | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Nitrite reductase | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | nitrogen dioxide | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | phytoremediation | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Rhaphiolepis umbellata (Thumb.) Makino | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | transgenic plant | |||||
| 資源タイプ | ||||||
| 資源 | http://purl.org/coar/resource_type/c_6501 | |||||
| タイプ | journal article | |||||
| 著者 |
Shigeto, Jun
× Shigeto, Jun× Yoshihara, Sanae× Adam, Suaad E.H.× Sueyoshi, Kuni× Sakamoto, Atsushi× Morikawa, Hiromichi× Takahashi, Misa |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Hypocotyl sections of Rhaphiolepis umbellata (Thunb.) Makino were transformed by Agrobacterium tumefaciens bearing a binary vector pIG121-AtNiR, which contains cDNA of the nitrite reductase gene from Arabidopsis thaliana (Atni) under the control of a modified cauliflower mosaic virus 35S promoter and chimeric hygromycin phosphotransferase gene (hph). A 4% of the hypocotyl explants transfected with Agrobacterium formed hygromycin resistant adventitious shoots, and most of them rooted upon root induction treatment. The presence and expression of the introduced transgene in putative transgenic plants (12 months after transfection) were respectively confirmed by polymerase chain reaction (PCR) analysis using primers specific to Atni and by western blot analysis using anti NiR antibody. A total of 37 transgenic plant lines were obtained. Plants 33 months after the transfection were fumigated with 200±50 ppb 15NO2 under the natural light for one week in the fumigation chamber in a confined glasshouse. The amount of total nitrogen derived from NO2 (reflecting uptake of NO2) and that of Kjeldahl nitrogen derived from NO2 (reflecting assimilation of NO2) were determined using a mass spectrometer. One of the 9 transgenic plants tested was 1.6–2.0 times higher both in the uptake and assimilation of NO2 than non-transformed wild-type ones. | |||||
| 言語 | en | |||||
| 書誌情報 |
en : Plant biotechnology 巻 23, 号 1, p. 111-116, 発行日 2006-03 |
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| 出版者 | ||||||
| 出版者 | 日本植物細胞分子生物学会 | |||||
| 言語 | ja | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1342-4580 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA11250821 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | http://doi.org/10.5511/plantbiotechnology.23.111 | |||||
| 出版タイプ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| 著者版フラグ | ||||||
| 値 | publisher | |||||
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Cite as
Shigeto, Jun, Yoshihara, Sanae, Adam, Suaad E.H., Sueyoshi, Kuni, Sakamoto, Atsushi, Morikawa, Hiromichi, Takahashi, Misa, 2006, Genetic engineering of nitrite reductase gene improves uptake and assimilation of nitrogen dioxide by Rhaphiolepis umbellata (Thunb.) Makino: 日本植物細胞分子生物学会, 111–116 p.
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