WEKO3
アイテム
Two-component high-affinity nitrate transport system in barley : Membrane localization, protein expression in roots and a direct protein-protein interaction
http://hdl.handle.net/10191/29870
http://hdl.handle.net/10191/29870253051c5-4e74-4823-b250-eeb2c619be3f
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
26_2_197-205.pdf (917.0 kB)
|
|
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2014-09-10 | |||||
| タイトル | ||||||
| タイトル | Two-component high-affinity nitrate transport system in barley : Membrane localization, protein expression in roots and a direct protein-protein interaction | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Barley | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | membrane localization | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | NAR2 | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | nitrate | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | NRT2 | |||||
| 資源タイプ | ||||||
| 資源 | http://purl.org/coar/resource_type/c_6501 | |||||
| タイプ | journal article | |||||
| 著者 |
Ishikawa, Shinji
× Ishikawa, Shinji× Ito, Yuka× Sato, Yuki× Fukaya, Yuka× Takahashi, Misa× Morikawa, Hiromichi× Ohtake, Norikuni× Ohyama, Takuji× Sueyoshi, Kuni |
|||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | A high affinity transport system (HATS) for nitrate in plants is operated by a two-component NRT2/NAR2 transport system. However, the regulation and localization of NRT2 and NAR2 at protein level are largely unknown and especially so in crop plant species. In this study with barley (Hordeum vulgare), membrane localization, protein expression in the roots, and a direct protein-protein interaction of HvNRT2 and HvNAR2 proteins were investigated. Immunochemical analysis showed that both HvNRT2 and HvNAR2 proteins were co-localized in the plasma membrane of the roots. Expression of HvNRT2 and HvNAR2 proteins was more strongly induced by treatment with higher concentrations of external nitrate, while HATS activity and transcripts for HvNRT2 and HvNAR2 were markedly repressed. An affinity column binding analysis using recombinant proteins suggests that the C-terminus of HvNRT2.1 is possibly involved in its binding to the HvNAR2.3 central region and that the Ser463 present in the HvNRT2.1 C-terminus plays a role in the binding ability. | |||||
| 言語 | en | |||||
| 書誌情報 |
en : Plant biotechnology 巻 26, 号 2, p. 197-205, 発行日 2009-03 |
|||||
| 出版者 | ||||||
| 出版者 | 日本植物細胞分子生物学会 | |||||
| 言語 | ja | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1342-4580 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA11250821 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | https://doi.org/10.5511/plantbiotechnology.26.197 | |||||
| 出版タイプ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| 著者版フラグ | ||||||
| 値 | publisher | |||||
