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  1. 0 資料タイプ別
  2. 03 紀要論文
  1. 250 大学院医歯学総合研究科(医)
  2. 20 紀要
  3. 02 新潟医学会雑誌
  4. 第100巻第11号

逆相 : 高速液体クロマトグラフィーを用いた微量アミノ酸分析によるArtemia salinaリボソーム小亜粒子蛋白質のアミノ酸組成 : ラットとの比較

http://hdl.handle.net/10191/35613
http://hdl.handle.net/10191/35613
fe25cb9d-8b63-4224-a8f6-18effbc6692c
名前 / ファイル ライセンス アクション
100(11)_652-668.pdf 100(11)_652-668.pdf (1.9 MB)
Item type 紀要論文 / Departmental Bulletin Paper(1)
公開日 2015-12-15
タイトル
タイトル 逆相 : 高速液体クロマトグラフィーを用いた微量アミノ酸分析によるArtemia salinaリボソーム小亜粒子蛋白質のアミノ酸組成 : ラットとの比較
タイトル
言語 en
タイトル 逆相 : 高速液体クロマトグラフィーを用いた微量アミノ酸分析によるArtemia salinaリボソーム小亜粒子蛋白質のアミノ酸組成 : ラットとの比較
言語
言語 jpn
キーワード
主題Scheme Other
主題 Artemia salina
キーワード
主題Scheme Other
主題 40S ribosomal protein
キーワード
主題Scheme Other
主題 two-dimentional acrylamide gel electrophoresis
キーワード
主題Scheme Other
主題 high-performance liquid chromatography
キーワード
主題Scheme Other
主題 dimethylaminoazobenzenesulfonyl derivative of amino acid
キーワード
主題Scheme Other
主題 アルテミアサリーナ
キーワード
主題Scheme Other
主題 リボソーム小亜粒子蛋白質
キーワード
主題Scheme Other
主題 二次元アクリルアミドゲル電気泳動
キーワード
主題Scheme Other
主題 高速液体クロマトグラフィー
キーワード
主題Scheme Other
主題 ダブシルアミノ酸
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ departmental bulletin paper
その他のタイトル
その他のタイトル Comparison of the amino acid compositions of individual 40 S ribosomal proteins between Artemia salina and rat liver by micro amino acid analyses with reverse-phase high-performance liquid chromatography
著者 小谷, スミ子

× 小谷, スミ子

WEKO 145922

小谷, スミ子

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著者別名
識別子 145923
識別子Scheme WEKO
姓名 Odani, Sumiko
抄録
内容記述タイプ Abstract
内容記述 To establish a method for the micro determination of the amino acid compositions of individual proteins of Artemia salina 40S ribosomal subunit and to compare the amino acid compositions with those of corresponding rat liver 40S proteins[Kenmochi, Tsurugi and Ogata(1981)J.Biochem., 89, 1293~1308], following experiments were carried out. The proteins of a small subunit of A. salina ribosomes were separated into twenty seven basic proteins by two-dimentional gel electrophoresis and eluted from the gels by diffusion into 70% formic acid. The eluted proteins were then purified by using size-exclusion high-performance liquid chromatography(HPLC). Amino acid analyses were performed by the preparation of dimethylaminoazobenzenesulfonyl(DABS)derivatives of amino acids, followed by reverse-phase HPLC. It was found that the amino acid compositions of horse heart cytochrome C and two A. salina 40S ribosomal proteins(S6 and S8)determined by the present methods were similar to those determined by the conventional amino acid analyzer. This method allowed to determine amino acids in pmole range. The amino acid compositions of A. salina and rat liver 40S ribosomal proteins were compaired and the similality of amino acid compositions of the corresponding proteins of rat liver and A. salina are evaluated by the method of Cornish-Bowden [Cornish-Bowden(1980)Anal. Biochem., 105, 233~238]. Ten pairs of proteins(S2, S3, S4, S6, S7, S8, S15a, S16, S17 and S18)are positive in the“strong”test, six pairs of proteins(S14, S15, S20, S23, S24 and S26)are positive in the“weak”test and seven pairs of proteins(S9, S10, S11, S13, S27, S27a and S28)are not related.
書誌情報 新潟医学会雑誌
en : 新潟医学会雑誌

巻 100, 号 11, p. 652-668, 発行日 1986-11
出版者
出版者 新潟医学会
ISSN
収録物識別子タイプ ISSN
収録物識別子 00290440
書誌レコードID
収録物識別子タイプ NCID
収録物識別子 AN00182415
著者版フラグ
値 publisher
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