{"_buckets": {"deposit": "0004b908-a2d4-4df0-abd6-014e071c9565"}, "_deposit": {"id": "24426", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "24426"}, "status": "published"}, "_oai": {"id": "oai:niigata-u.repo.nii.ac.jp:00024426", "sets": ["456", "1240"]}, "item_7_alternative_title_1": {"attribute_name": "その他のタイトル", "attribute_value_mlt": [{"subitem_alternative_title": "Comparison of the amino acid compositions of individual 40 S ribosomal proteins between Artemia salina and rat liver by micro amino acid analyses with reverse-phase high-performance liquid chromatography"}]}, "item_7_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "1986-11", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "11", "bibliographicPageEnd": "668", "bibliographicPageStart": "652", "bibliographicVolumeNumber": "100", "bibliographic_titles": [{"bibliographic_title": "新潟医学会雑誌"}, {"bibliographic_title": "新潟医学会雑誌", "bibliographic_titleLang": "en"}]}]}, "item_7_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "To establish a method for the micro determination of the amino acid compositions of individual proteins of Artemia salina 40S ribosomal subunit and to compare the amino acid compositions with those of corresponding rat liver 40S proteins[Kenmochi, Tsurugi and Ogata（1981）J.Biochem., 89, 1293～1308], following experiments were carried out. The proteins of a small subunit of A. salina ribosomes were separated into twenty seven basic proteins by two-dimentional gel electrophoresis and eluted from the gels by diffusion into 70% formic acid. The eluted proteins were then purified by using size-exclusion high-performance liquid chromatography（HPLC）. Amino acid analyses were performed by the preparation of dimethylaminoazobenzenesulfonyl（DABS）derivatives of amino acids, followed by reverse-phase HPLC. It was found that the amino acid compositions of horse heart cytochrome C and two A. salina 40S ribosomal proteins（S6 and S8）determined by the present methods were similar to those determined by the conventional amino acid analyzer. This method allowed to determine amino acids in pmole range. The amino acid compositions of A. salina and rat liver 40S ribosomal proteins were compaired and the similality of amino acid compositions of the corresponding proteins of rat liver and A. salina are evaluated by the method of Cornish-Bowden [Cornish-Bowden（1980）Anal. Biochem., 105, 233～238]. Ten pairs of proteins（S2, S3, S4, S6, S7, S8, S15a, S16, S17 and S18）are positive in the“strong”test, six pairs of proteins（S14, S15, S20, S23, S24 and S26）are positive in the“weak”test and seven pairs of proteins（S9, S10, S11, S13, S27, S27a and S28）are not related.", "subitem_description_type": "Abstract"}]}, "item_7_full_name_3": {"attribute_name": "著者別名", "attribute_value_mlt": [{"nameIdentifiers": [{"nameIdentifier": "145923", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "Odani, Sumiko"}]}]}, "item_7_publisher_7": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "新潟医学会"}]}, "item_7_select_19": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_7_source_id_11": {"attribute_name": "書誌レコードID", "attribute_value_mlt": [{"subitem_source_identifier": "AN00182415", "subitem_source_identifier_type": "NCID"}]}, "item_7_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "00290440", "subitem_source_identifier_type": "ISSN"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "小谷, スミ子"}], "nameIdentifiers": [{"nameIdentifier": "145922", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-08-19"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "100(11)_652-668.pdf", "filesize": [{"value": "1.9 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 1900000.0, "url": {"label": "100(11)_652-668.pdf", "url": "https://niigata-u.repo.nii.ac.jp/record/24426/files/100(11)_652-668.pdf"}, "version_id": "c8fb28d1-0303-44e2-8f92-aa6f65651b42"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "Artemia salina", "subitem_subject_scheme": "Other"}, {"subitem_subject": "40S ribosomal protein", "subitem_subject_scheme": "Other"}, {"subitem_subject": "two-dimentional acrylamide gel electrophoresis", "subitem_subject_scheme": "Other"}, {"subitem_subject": "high-performance liquid chromatography", "subitem_subject_scheme": "Other"}, {"subitem_subject": "dimethylaminoazobenzenesulfonyl derivative of amino acid", "subitem_subject_scheme": "Other"}, {"subitem_subject": "アルテミアサリーナ", "subitem_subject_scheme": "Other"}, {"subitem_subject": "リボソーム小亜粒子蛋白質", "subitem_subject_scheme": "Other"}, {"subitem_subject": "二次元アクリルアミドゲル電気泳動", "subitem_subject_scheme": "Other"}, {"subitem_subject": "高速液体クロマトグラフィー", "subitem_subject_scheme": "Other"}, {"subitem_subject": "ダブシルアミノ酸", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "jpn"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "逆相 : 高速液体クロマトグラフィーを用いた微量アミノ酸分析によるArtemia salinaリボソーム小亜粒子蛋白質のアミノ酸組成 : ラットとの比較", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "逆相 : 高速液体クロマトグラフィーを用いた微量アミノ酸分析によるArtemia salinaリボソーム小亜粒子蛋白質のアミノ酸組成 : ラットとの比較"}, {"subitem_title": "逆相 : 高速液体クロマトグラフィーを用いた微量アミノ酸分析によるArtemia salinaリボソーム小亜粒子蛋白質のアミノ酸組成 : ラットとの比較", "subitem_title_language": "en"}]}, "item_type_id": "7", "owner": "1", "path": ["456", "1240"], "permalink_uri": "http://hdl.handle.net/10191/35613", "pubdate": {"attribute_name": "公開日", "attribute_value": "2015-12-15"}, "publish_date": "2015-12-15", "publish_status": "0", "recid": "24426", "relation": {}, "relation_version_is_last": true, "title": ["逆相 : 高速液体クロマトグラフィーを用いた微量アミノ酸分析によるArtemia salinaリボソーム小亜粒子蛋白質のアミノ酸組成 : ラットとの比較"], "weko_shared_id": null}