{"_buckets": {"deposit": "0004b908-a2d4-4df0-abd6-014e071c9565"}, "_deposit": {"id": "24426", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "24426"}, "status": "published"}, "_oai": {"id": "oai:niigata-u.repo.nii.ac.jp:00024426"}, "item_7_alternative_title_1": {"attribute_name": "\u305d\u306e\u4ed6\u306e\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_alternative_title": "Comparison of the amino acid compositions of individual 40 S ribosomal proteins between Artemia salina and rat liver by micro amino acid analyses with reverse-phase high-performance liquid chromatography"}]}, "item_7_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "1986-11", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "11", "bibliographicPageEnd": "668", "bibliographicPageStart": "652", "bibliographicVolumeNumber": "100", "bibliographic_titles": [{"bibliographic_title": "\u65b0\u6f5f\u533b\u5b66\u4f1a\u96d1\u8a8c"}, {"bibliographic_title": "\u65b0\u6f5f\u533b\u5b66\u4f1a\u96d1\u8a8c", "bibliographic_titleLang": "en"}]}]}, "item_7_description_4": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "To establish a method for the micro determination of the amino acid compositions of individual proteins of Artemia salina 40S ribosomal subunit and to compare the amino acid compositions with those of corresponding rat liver 40S proteins[Kenmochi, Tsurugi and Ogata\uff081981\uff09J.Biochem., 89, 1293\uff5e1308], following experiments were carried out. The proteins of a small subunit of A. salina ribosomes were separated into twenty seven basic proteins by two-dimentional gel electrophoresis and eluted from the gels by diffusion into 70% formic acid. The eluted proteins were then purified by using size-exclusion high-performance liquid chromatography\uff08HPLC\uff09. Amino acid analyses were performed by the preparation of dimethylaminoazobenzenesulfonyl\uff08DABS\uff09derivatives of amino acids, followed by reverse-phase HPLC. It was found that the amino acid compositions of horse heart cytochrome C and two A. salina 40S ribosomal proteins\uff08S6 and S8\uff09determined by the present methods were similar to those determined by the conventional amino acid analyzer. This method allowed to determine amino acids in pmole range. The amino acid compositions of A. salina and rat liver 40S ribosomal proteins were compaired and the similality of amino acid compositions of the corresponding proteins of rat liver and A. salina are evaluated by the method of Cornish-Bowden [Cornish-Bowden\uff081980\uff09Anal. Biochem., 105, 233\uff5e238]. Ten pairs of proteins\uff08S2, S3, S4, S6, S7, S8, S15a, S16, S17 and S18\uff09are positive in the\u201cstrong\u201dtest, six pairs of proteins\uff08S14, S15, S20, S23, S24 and S26\uff09are positive in the\u201cweak\u201dtest and seven pairs of proteins\uff08S9, S10, S11, S13, S27, S27a and S28\uff09are not related.", "subitem_description_type": "Abstract"}]}, "item_7_full_name_3": {"attribute_name": "\u8457\u8005\u5225\u540d", "attribute_value_mlt": [{"nameIdentifiers": [{"nameIdentifier": "145923", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "Odani, Sumiko"}]}]}, "item_7_publisher_7": {"attribute_name": "\u51fa\u7248\u8005", "attribute_value_mlt": [{"subitem_publisher": "\u65b0\u6f5f\u533b\u5b66\u4f1a"}]}, "item_7_select_19": {"attribute_name": "\u8457\u8005\u7248\u30d5\u30e9\u30b0", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_7_source_id_11": {"attribute_name": "\u66f8\u8a8c\u30ec\u30b3\u30fc\u30c9ID", "attribute_value_mlt": [{"subitem_source_identifier": "AN00182415", "subitem_source_identifier_type": "NCID"}]}, "item_7_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "00290440", "subitem_source_identifier_type": "ISSN"}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "\u5c0f\u8c37, \u30b9\u30df\u5b50"}], "nameIdentifiers": [{"nameIdentifier": "145922", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-08-19"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "100(11)_652-668.pdf", "filesize": [{"value": "1.9 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 1900000.0, "url": {"label": "100(11)_652-668.pdf", "url": "https://niigata-u.repo.nii.ac.jp/record/24426/files/100(11)_652-668.pdf"}, "version_id": "c8fb28d1-0303-44e2-8f92-aa6f65651b42"}]}, "item_keyword": {"attribute_name": "\u30ad\u30fc\u30ef\u30fc\u30c9", "attribute_value_mlt": [{"subitem_subject": "Artemia salina", "subitem_subject_scheme": "Other"}, {"subitem_subject": "40S ribosomal protein", "subitem_subject_scheme": "Other"}, {"subitem_subject": "two-dimentional acrylamide gel electrophoresis", "subitem_subject_scheme": "Other"}, {"subitem_subject": "high-performance liquid chromatography", "subitem_subject_scheme": "Other"}, {"subitem_subject": "dimethylaminoazobenzenesulfonyl derivative of amino acid", "subitem_subject_scheme": "Other"}, {"subitem_subject": "\u30a2\u30eb\u30c6\u30df\u30a2\u30b5\u30ea\u30fc\u30ca", "subitem_subject_scheme": "Other"}, {"subitem_subject": "\u30ea\u30dc\u30bd\u30fc\u30e0\u5c0f\u4e9c\u7c92\u5b50\u86cb\u767d\u8cea", 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