{"_buckets": {"deposit": "7b5beac5-d8ad-4264-b455-4985575dd0a2"}, "_deposit": {"id": "21952", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "21952"}, "status": "published"}, "_oai": {"id": "oai:niigata-u.repo.nii.ac.jp:00021952"}, "item_7_alternative_title_1": {"attribute_name": "\u305d\u306e\u4ed6\u306e\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_alternative_title": "Expression of c-erbB-2 mRNA in Cultured Human Cancer Cell Lines and Characterization of c-erbB-2-Related Protein"}]}, "item_7_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "1990-10", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "10", "bibliographicPageEnd": "844", "bibliographicPageStart": "835", "bibliographicVolumeNumber": "104", "bibliographic_titles": [{"bibliographic_title": "\u65b0\u6f5f\u533b\u5b66\u4f1a\u96d1\u8a8c"}, {"bibliographic_title": "\u65b0\u6f5f\u533b\u5b66\u4f1a\u96d1\u8a8c", "bibliographic_titleLang": "en"}]}]}, "item_7_description_4": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "Expression of c-erbB-2 mRNA was examined in 33 cultured human cell lines using two synthetic oligonucleotide probes hybridizable to c-erbB-2 mRNA at the portions cording the extracellular and transmembrane domains. The mRNA with molecular size of 5.0 kb was detected in 28 out of 33 cell lines examined, suggesting that cancer cells frequently produce c-erbB-2 protein. Additionally, in three cancer cell lines expressing a large amount of the 5.0 kb mRNA, an aberrant mRNA with molecular size of 2.5 kb was detected, which reacted with the probe corresponding to the extracellular domain, but did not with another probe. The biochemical characteristics of c-erbB-2 protein were examined in a gastric carcinoma cell line, MKN 7 cells, expressing a large amount of c-erbB-2 mRNA. When the cells were radiolabelled with ^\u003c32\u003ePi, lysed and then labelled proteins were immunoprecipitated with three different antibodies recognizing extracellular, kinase and carboxyl-terminal domains, phosphorylated c-erbB-2 protein with molecular size of 185 kD (p185) was immunoprecipitated by all of these three antibodies. When the cells were radiolabelled with (^\u003c35\u003eS) cysteine, p185 was detected with three antibodies, moreover, a large amount of (^\u003c35\u003eS)-labelled protein with molecular size of 95 kD (p95) was also detected by using the antibody recognizing the extracellular domain. Other two antibodies did not immunoprocipitate this molecule, suggesting that p95 possesses the structure identical to the extracellular domain of p185. Pulse chase experiments suggest that p95 is not likely to be a degenerating protein of p185. Accordingly, it is reasonable to postulate that two types of c-erbB-2-related protein were present in MKN7 cells with molecular size heterogeneity. Further studies are required to clarify the actual biological roles of c-erbB-2 protein produced by cancer cells.", "subitem_description_type": "Abstract"}]}, "item_7_full_name_3": {"attribute_name": "\u8457\u8005\u5225\u540d", "attribute_value_mlt": [{"nameIdentifiers": [{"nameIdentifier": "133579", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "Hitomi, Jiro"}]}]}, "item_7_publisher_7": {"attribute_name": "\u51fa\u7248\u8005", "attribute_value_mlt": [{"subitem_publisher": "\u65b0\u6f5f\u533b\u5b66\u4f1a"}]}, "item_7_select_19": {"attribute_name": "\u8457\u8005\u7248\u30d5\u30e9\u30b0", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_7_source_id_11": {"attribute_name": "\u66f8\u8a8c\u30ec\u30b3\u30fc\u30c9ID", "attribute_value_mlt": [{"subitem_source_identifier": "AN00182415", "subitem_source_identifier_type": "NCID"}]}, "item_7_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "00290440", "subitem_source_identifier_type": "ISSN"}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "\u4eba\u898b, \u6b21\u90ce"}], "nameIdentifiers": [{"nameIdentifier": "133578", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-08-16"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "104(10)_835-844.pdf", "filesize": [{"value": "2.9 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 2900000.0, "url": {"label": "104(10)_835-844.pdf", "url": "https://niigata-u.repo.nii.ac.jp/record/21952/files/104(10)_835-844.pdf"}, "version_id": "e0f932db-e6f6-4b3f-8585-7677139ebea9"}]}, "item_keyword": {"attribute_name": "\u30ad\u30fc\u30ef\u30fc\u30c9", "attribute_value_mlt": [{"subitem_subject": "c-erbB-2", "subitem_subject_scheme": "Other"}, {"subitem_subject": "growth factor", "subitem_subject_scheme": "Other"}, {"subitem_subject": "receptor", "subitem_subject_scheme": "Other"}, {"subitem_subject": "cancer", "subitem_subject_scheme": "Other"}, {"subitem_subject": "oncogene", "subitem_subject_scheme": "Other"}, {"subitem_subject": "\u5897\u6b96\u56e0\u5b50", "subitem_subject_scheme": "Other"}, {"subitem_subject": "\u30ec\u30bb\u30d7\u30bf\u30fc", "subitem_subject_scheme": "Other"}, {"subitem_subject": "\u304c\u3093", "subitem_subject_scheme": "Other"}, {"subitem_subject": "\u304c\u3093\u907a\u4f1d\u5b50", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "jpn"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "\u30d2\u30c8\u304c\u3093\u57f9\u990a\u7d30\u80de\u682a\u306b\u304a\u3051\u308b c-erbB-2 mRNA\u306e\u767a\u73fe\u304a\u3088\u3073 c-erbB-2\u95a2\u9023\u86cb\u767d\u306e\u6027\u72b6", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "\u30d2\u30c8\u304c\u3093\u57f9\u990a\u7d30\u80de\u682a\u306b\u304a\u3051\u308b c-erbB-2 mRNA\u306e\u767a\u73fe\u304a\u3088\u3073 c-erbB-2\u95a2\u9023\u86cb\u767d\u306e\u6027\u72b6"}, {"subitem_title": "\u30d2\u30c8\u304c\u3093\u57f9\u990a\u7d30\u80de\u682a\u306b\u304a\u3051\u308b c-erbB-2 mRNA\u306e\u767a\u73fe\u304a\u3088\u3073 c-erbB-2\u95a2\u9023\u86cb\u767d\u306e\u6027\u72b6", "subitem_title_language": "en"}]}, "item_type_id": "7", "owner": "1", "path": ["453/456", "471/537/538/1193"], "permalink_uri": "http://hdl.handle.net/10191/34969", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_value": "2015-12-04"}, "publish_date": "2015-12-04", "publish_status": "0", "recid": "21952", "relation": {}, "relation_version_is_last": true, "title": ["\u30d2\u30c8\u304c\u3093\u57f9\u990a\u7d30\u80de\u682a\u306b\u304a\u3051\u308b c-erbB-2 mRNA\u306e\u767a\u73fe\u304a\u3088\u3073 c-erbB-2\u95a2\u9023\u86cb\u767d\u306e\u6027\u72b6"], "weko_shared_id": null}