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Nuclear Transport of the Major Capsid Proteins Is Essential for Adeno-Associated Virus Capsid Formation
http://hdl.handle.net/10191/25657
http://hdl.handle.net/10191/25657a035fdd5-49c7-4956-bdfe-7a056cb859d5
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
73_9_7912-7915.pdf (537.3 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2014-02-19 | |||||
| タイトル | ||||||
| タイトル | Nuclear Transport of the Major Capsid Proteins Is Essential for Adeno-Associated Virus Capsid Formation | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源 | http://purl.org/coar/resource_type/c_6501 | |||||
| タイプ | journal article | |||||
| 著者 |
Hoque, Mainul
× Hoque, Mainul× Ishizu, Ken-Ichiro× Matsumoto, Akiko× Han, Song-Iee× Arisaka, Fumio× Takayama, Makoto× Suzuki, Kenji× Kato, Kenzo× Kanda, Tadahito× Watanabe, Hajime× Handa, Hiroshi |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Adeno-associated virus capsids are composed of three proteins, VP1, VP2, and VP3. Although VP1 is necessary for viral infection, it is not essential for capsid formation. The other capsid proteins, VP2 and VP3, are sufficient for capsid formation, but the functional roles of each protein are still not well understood. By analyzing a series of deletion mutants of VP2, we identified a region necessary for nuclear transfer of VP2 and found that the efficiency of nuclear localization of the capsid proteins and the efficiency of virus-like particle (VLP) formation correlated well. To confirm the importance of the nuclear localization of the capsid proteins, we fused the nuclear localization signal of simian virus 40 large T antigen to VP3 protein. We show that this fusion protein could form VLP, indicating that the VP2-specific region located on the N-terminal side of the protein is not structurally required. This finding suggests that VP3 has sufficient information for VLP formation and that VP2 is necessary only for nuclear transfer of the capsid proteins. | |||||
| 言語 | en | |||||
| 書誌情報 |
en : Journal of Virology 巻 73, 号 9, p. 7912-7915, 発行日 1999-09 |
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| 出版者 | ||||||
| 出版者 | American Society for Microbiology | |||||
| 言語 | en | |||||
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| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0022-538X | |||||
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| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA00708779 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | http://doi.org/10.1128/jvi.73.9.7912-7915.1999 | |||||
| 権利 | ||||||
| 権利情報 | Copyright (C) 1999, American Society for Microbiology | |||||
| 出版タイプ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| 著者版フラグ | ||||||
| 値 | publisher | |||||
